| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 72,273 | G→A | 20.8% | Q59Q (CAG→CAA) | ilvH → | acetolactate synthase III, thiamin‑dependent, small subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 72,273 | 0 | G | A | 20.8% | 117.8 / 20.2 | 81 | Q59Q (CAG→CAA) | ilvH | acetolactate synthase III, thiamin‑dependent, small subunit |
| Reads supporting (aligned to +/- strand): ref base G (17/47); new base A (11/6); total (28/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.10e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01 | |||||||||||
CCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTG > minE/72205‑72319 | ccAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAg < 1:2371562/69‑1 (MQ=255)ccAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAg < 1:2955671/69‑1 (MQ=255)ccAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAg < 1:1383938/69‑1 (MQ=255) ccGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCa > 1:528761/1‑68 (MQ=255) tATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTAcaca < 1:3306126/69‑1 (MQ=255) tATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTAcaca < 1:232615/69‑1 (MQ=255) tATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTAcaca < 1:3046537/69‑1 (MQ=255) tATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTAcaca < 1:3058856/69‑1 (MQ=255) tATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTAcaca < 1:1652068/69‑1 (MQ=255) tATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTAcaca < 1:1033627/69‑1 (MQ=255) tATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTAcaca < 1:792328/69‑1 (MQ=255) tATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTAcaca < 1:868278/69‑1 (MQ=255) tATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAAGTAcaca < 1:436352/69‑1 (MQ=255) aTCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCa < 1:1976093/60‑1 (MQ=255) aTCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCa < 1:1132875/60‑1 (MQ=255) aTCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCa < 1:2558096/60‑1 (MQ=255) aTCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCa < 1:2073134/60‑1 (MQ=255) aTCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCa < 1:2049829/60‑1 (MQ=255) aTCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCa < 1:2727572/60‑1 (MQ=255) aTCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCa < 1:710663/60‑1 (MQ=255) aTCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCa < 1:3375350/60‑1 (MQ=255) aTCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCa < 1:696290/60‑1 (MQ=255) aTCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCa < 1:1478889/60‑1 (MQ=255) aTCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTAcaca < 1:1147301/68‑1 (MQ=255) aTCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACaa > 1:3000425/1‑69 (MQ=255) cgcgTATGACCATTCAGACCGTGGGCGATGAAAAAGTGCTTGAGCAAATCGAAAAGCAATTACACaagc > 1:617625/1‑67 (MQ=255) cgcgTATGACCATTCAGACCGTGGGCGATGAAAAAGTGCTTGAGCAAATCGAAAAGCAATTACACaagc > 1:3035506/1‑67 (MQ=255) cgcgTATGACCATTCAGACCGTGGGCGATGAAAAAGTGCTTGAGCAAATCGAAAAGCAATTACACaagc > 1:2701975/1‑67 (MQ=255) cgcgTATGACCATTCAGACCGTGGGCGATGAAAAAGTGCTTGAGCAAATCGAAAAGCAATTACACaagc > 1:917746/1‑67 (MQ=255) cgcgTATGACCATTCAGACCGTGGGCGATGAAAAAGTGCTTGAGCAAATCGAAAAGCAATTACACaagc > 1:2521235/1‑67 (MQ=255) cgcgTATGACCATTCAGACCGTGGGCGATGAAAAAGTGCTTGAGCAAATCGAAAAGCAATTACACaagc > 1:2458665/1‑67 (MQ=255) cgcgTATGACCATTCAGACCGTGGGCGATGAAAAAGTGCTTGAGCAAATCGAAAAGCAATTACACaagc > 1:2390363/1‑67 (MQ=255) cgcgTATGACCATTCAGACCGTGGGCGATGAAAAAGTGCTTGAGCAAATCGAAAAGCAATTACACaagc > 1:1322115/1‑67 (MQ=255) cgcgTATGACCATTCAGACCGTGGGCGATGAAAAAGTGCTTGAGCAAATCGAAAAGCAATTACACaagc > 1:1690102/1‑67 (MQ=255) cgcgTATGACCATTCAGACCGTGGGCGATGAAAAAGTGCTTGAGCAAATCGAAAAGCAATTACACaagc > 1:1673635/1‑67 (MQ=255) cgcgTATGACCATTCAGACCGTGGGCGATGAAAAAGTGCTTGAGCAAATCGAAAAGCAATTACACaagc > 1:1672489/1‑67 (MQ=255) cAGACCGTGGGCGATGTAAAAGTACTTGAGCAGATCGAAAAGc < 1:1256888/43‑1 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAg > 1:2721919/1‑42 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGc < 1:2932216/43‑1 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGc > 1:101736/1‑43 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGc > 1:1864476/1‑43 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGc < 1:3226127/43‑1 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGc > 1:324354/1‑43 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGc > 1:3286275/1‑43 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGc > 1:3386681/1‑43 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGc > 1:3411051/1‑43 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGc < 1:365078/43‑1 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGc < 1:1547050/43‑1 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGc > 1:531409/1‑43 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGc < 1:577490/43‑1 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGc < 1:2857629/43‑1 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGc > 1:2450598/1‑43 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGc > 1:2795841/1‑43 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGc > 1:2192624/1‑43 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGc < 1:2525912/43‑1 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGc > 1:2016129/1‑43 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAAt < 1:290609/46‑1 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAAt < 1:1217100/46‑1 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAAt < 1:823788/46‑1 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAAt < 1:780732/46‑1 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAAt < 1:522526/46‑1 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAAt < 1:2185648/46‑1 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAAt < 1:2627929/46‑1 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAAt < 1:1590033/46‑1 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAAt < 1:2685743/46‑1 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAAt < 1:2709957/46‑1 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAAt < 1:1996868/46‑1 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAAt < 1:1918805/46‑1 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAAt < 1:305974/46‑1 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAAt < 1:2750901/46‑1 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTAcaca > 1:1221136/1‑52 (MQ=255) cAGACCGTGGGCGATGAAAAAGTACTTCAGCAGATCGAAAAGc < 1:68660/43‑1 (MQ=255) cAGAACGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAAt < 1:1344154/46‑1 (MQ=255) ccGTGGGCGATGAAAAAGTGCTTGAGCAAATCGAAAAGCAATTa < 1:2821057/44‑1 (MQ=38) ccGTGGGCGATGAAAAAGTGCTTGAGCAAATCGAAAAGCAATTa < 1:2461551/44‑1 (MQ=38) ccGTGGGCGATGAAAAAGTGCTTGAGCAAATCGAAAAGCAATTa < 1:1489222/44‑1 (MQ=38) ccGTGGGCGATGAAAAAGTGCTTGAGCAAATCGAAAAGCAATTa < 1:2737230/44‑1 (MQ=38) ccGTGGGCGATGAAAAAGTGCTTGAGCAAATCGAAAAGCAATTa < 1:380561/44‑1 (MQ=38) ccGTGGGCGATGAAAAAGTGCTTGAGCAAATCGAAAAGCAATTa < 1:2032478/44‑1 (MQ=38) ggCGATGAAAAAGTACTTGAGCAGCTCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCgtga > 1:2567327/1‑67 (MQ=255) ggCGATGAAAAAGTACTTGAGCAGATCGAAAAGCa < 1:3139828/35‑1 (MQ=255) ggCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCgtga > 1:297367/1‑67 (MQ=255) cGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCgtgagtg < 1:2525366/68‑1 (MQ=255) cGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCgtgagtg < 1:2311116/68‑1 (MQ=255) | CCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTG > minE/72205‑72319 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |