| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 715,819 | A→G | 28.9% | G112G (GGT→GGC) | mdtH ← | predicted drug efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 715,819 | 0 | A | G | 28.9% | 32.8 / 13.9 | 38 | G112G (GGT→GGC) | mdtH | predicted drug efflux system |
| Reads supporting (aligned to +/- strand): ref base A (14/13); new base G (8/3); total (22/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 | |||||||||||
GCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAA > minE/715758‑715887 | gctgctGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTc < 1:1313315/69‑1 (MQ=255)gctgctGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTc < 1:2954352/69‑1 (MQ=255)gctgctGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTc < 1:1444978/69‑1 (MQ=255) tgctgTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTccc < 1:279340/69‑1 (MQ=255) tgctgTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTccc < 1:2544816/69‑1 (MQ=255) tgctgTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTccc < 1:2489028/69‑1 (MQ=255) ttAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCAt > 1:3054358/1‑68 (MQ=255) ttCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCga > 1:791692/1‑53 (MQ=255) ttCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCga > 1:1403184/1‑53 (MQ=255) ttCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCga > 1:1491791/1‑53 (MQ=255) ttCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCga > 1:641075/1‑53 (MQ=255) ttCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCAACGAGTCCCga > 1:2879006/1‑53 (MQ=255) tCACCACCAGCGCCGAACGCGTAGGATCAAACAACGTGCCGCCGAGTCCCAAAAGCAGGCATGAAAAc > 1:375638/1‑68 (MQ=255) tCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGaa < 1:1907210/65‑1 (MQ=255) tCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGaa < 1:654127/65‑1 (MQ=255) tCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAAc > 1:441052/1‑68 (MQ=255) tCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAAc > 1:757332/1‑68 (MQ=255) tCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAAc > 1:1409528/1‑68 (MQ=255) tCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAAc > 1:1339182/1‑68 (MQ=255) tCACCACCAGCGCCGAACGCGGAGGATCAAACAACGTGCCGCCGAGTCCCGAAAGCAGGCATGAAAAc > 1:1656413/1‑68 (MQ=255) tCACCACCAGCGCCGAACGCGGAGGATCAAACAACGTGCCGCCGAGTCCCGAAAGCAGGCATGAAAAc > 1:508763/1‑68 (MQ=255) tCACCACCAGCGCCGAACGCGGAGGATCAAACAACGTGCCGCCGAGTCCCGAAAGCAGGCATGAAAAc > 1:1954332/1‑68 (MQ=255) tCACCACCAGCGCCGAACGCGGAGGATCAAACAACGTGCCGCCGAGTCCCGAAAGCAGGCATGAAAAc > 1:3387286/1‑68 (MQ=255) tCACCACCAGCGCCGAACGCGGAGGATCAAACAACGTGCCGCCGAGTCCCGAAAGCAGGCATGAAAAc > 1:2335429/1‑68 (MQ=255) tCACCACCAGCGCCGAACGCGGAGGATCAAACAACGTGCCGCCGAGTCCCGAAAGCAGGCATGAAAAc > 1:3219828/1‑68 (MQ=255) tCACCACCAGCGCCGAACGCGGAGGATCAAACAACGTGCCGCCGAGTCCCGAAAGCAGGCATGAAAAc > 1:1431507/1‑68 (MQ=255) aaCGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGtt < 1:2403148/69‑1 (MQ=255) aaCGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGtt < 1:2580304/69‑1 (MQ=255) aCGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGGCACGGTTc > 1:811836/1‑69 (MQ=255) aCGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGtt < 1:1941055/68‑1 (MQ=255) aCGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTc > 1:1089102/1‑69 (MQ=255) aCGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTc > 1:1777493/1‑69 (MQ=255) aCGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTc > 1:1447844/1‑69 (MQ=255) cggATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGc < 1:261376/69‑1 (MQ=255) cggATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGc < 1:1363302/69‑1 (MQ=255) acaacGTGCCGCCGAGTCCCGAAAGCAGGCATGAAAACCACAGTAGCCACGGTTCGTGGGCGATACCCa < 1:2284287/69‑1 (MQ=255) acaacGTGCCGCCGAGTCCCGAAAGCAGGCATGAAAACCACAGTAGCCACGGTTCGTGGGCGATACCCa < 1:1515507/69‑1 (MQ=255) caacGTGCCGCCGAGTCCCGAAAGCAGGCATGAAAACCACAGTAGCCACGGTTCGTGGGCGATACCCa < 1:1850691/68‑1 (MQ=255) aCCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGaa < 1:2025629/69‑1 (MQ=255) aCCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGCTCGTGGGCGATACCCATTGTGGCGaa < 1:962136/69‑1 (MQ=255) | GCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAA > minE/715758‑715887 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |