| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 715,831 | G→A | 38.6% | L108L (CTC→CTT) | mdtH ← | predicted drug efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 715,831 | 0 | G | A | 38.6% | 12.4 / 17.3 | 31 | L108L (CTC→CTT) | mdtH | predicted drug efflux system |
| Reads supporting (aligned to +/- strand): ref base G (9/10); new base A (8/4); total (17/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAA > minE/715773‑715887 | ttAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCAt > 1:3054358/1‑68 (MQ=255) tCACCACCAGCGCCGAACGCGTAGGATCAAACAACGTGCCGCCGAGTCCCAAAAGCAGGCATGAAAAc > 1:375638/1‑68 (MQ=255) tCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGaa < 1:1907210/65‑1 (MQ=255) tCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGaa < 1:654127/65‑1 (MQ=255) tCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAAc > 1:757332/1‑68 (MQ=255) tCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAAc > 1:441052/1‑68 (MQ=255) tCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAAc > 1:1409528/1‑68 (MQ=255) tCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAAc > 1:1339182/1‑68 (MQ=255) tCACCACCAGCGCCGAACGCGGAGGATCAAACAACGTGCCGCCGAGTCCCGAAAGCAGGCATGAAAAc > 1:508763/1‑68 (MQ=255) tCACCACCAGCGCCGAACGCGGAGGATCAAACAACGTGCCGCCGAGTCCCGAAAGCAGGCATGAAAAc > 1:1431507/1‑68 (MQ=255) tCACCACCAGCGCCGAACGCGGAGGATCAAACAACGTGCCGCCGAGTCCCGAAAGCAGGCATGAAAAc > 1:1656413/1‑68 (MQ=255) tCACCACCAGCGCCGAACGCGGAGGATCAAACAACGTGCCGCCGAGTCCCGAAAGCAGGCATGAAAAc > 1:3387286/1‑68 (MQ=255) tCACCACCAGCGCCGAACGCGGAGGATCAAACAACGTGCCGCCGAGTCCCGAAAGCAGGCATGAAAAc > 1:3219828/1‑68 (MQ=255) tCACCACCAGCGCCGAACGCGGAGGATCAAACAACGTGCCGCCGAGTCCCGAAAGCAGGCATGAAAAc > 1:1954332/1‑68 (MQ=255) tCACCACCAGCGCCGAACGCGGAGGATCAAACAACGTGCCGCCGAGTCCCGAAAGCAGGCATGAAAAc > 1:2335429/1‑68 (MQ=255) aaCGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGtt < 1:2580304/69‑1 (MQ=255) aaCGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGtt < 1:2403148/69‑1 (MQ=255) aCGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGGCACGGTTc > 1:811836/1‑69 (MQ=255) aCGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGtt < 1:1941055/68‑1 (MQ=255) aCGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTc > 1:1777493/1‑69 (MQ=255) aCGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTc > 1:1447844/1‑69 (MQ=255) aCGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTc > 1:1089102/1‑69 (MQ=255) cggATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGc < 1:261376/69‑1 (MQ=255) cggATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGc < 1:1363302/69‑1 (MQ=255) acaacGTGCCGCCGAGTCCCGAAAGCAGGCATGAAAACCACAGTAGCCACGGTTCGTGGGCGATACCCa < 1:2284287/69‑1 (MQ=255) acaacGTGCCGCCGAGTCCCGAAAGCAGGCATGAAAACCACAGTAGCCACGGTTCGTGGGCGATACCCa < 1:1515507/69‑1 (MQ=255) caacGTGCCGCCGAGTCCCGAAAGCAGGCATGAAAACCACAGTAGCCACGGTTCGTGGGCGATACCCa < 1:1850691/68‑1 (MQ=255) ccgccGAGTCCCGAAAGCAGGCATGAAAACCACAGTAGCCACGGTTCGTGGGCGATACCCa < 1:131653/58‑1 (MQ=255) aCCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGaa < 1:2025629/69‑1 (MQ=255) aCCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGCTCGTGGGCGATACCCATTGTGGCGaa < 1:962136/69‑1 (MQ=255) ccGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGaa < 1:768001/68‑1 (MQ=255) | TTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAA > minE/715773‑715887 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |