| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 716,482 | T→G | 35.2% | L31L (CTT→CTG) | rimJ → | ribosomal‑protein‑S5‑alanine N‑acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 716,482 | 0 | T | G | 35.2% | 85.6 / 108.2 | 128 | L31L (CTT→CTG) | rimJ | ribosomal‑protein‑S5‑alanine N‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (14/69); new base G (27/18); total (41/87) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.09e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
Only 100 of 128 total aligned reads displayed.
TGCGCTTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCC > minE/716421‑716546 | tGCGCTTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGatt > 1:2749496/1‑69 (MQ=255)tGCGCTTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGatt > 1:541945/1‑69 (MQ=255)tGCGCTTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGatt > 1:248502/1‑69 (MQ=255) aaCCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTGGCGGAttatta > 1:898496/1‑66 (MQ=255) aaCCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTGGCGGAttatta > 1:179016/1‑66 (MQ=255) aaCCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTGGCGGAttatta > 1:803331/1‑66 (MQ=255) aaCCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTGGCGGAttatta > 1:1417832/1‑66 (MQ=255) aaCCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTGGCGGAttatta > 1:1971038/1‑66 (MQ=255) aaCCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTGGCGGAttatta > 1:2056170/1‑66 (MQ=255) aaCCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTGGCGGAttatta > 1:404709/1‑66 (MQ=255) aaCCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTGGCGGAttatta > 1:3410549/1‑66 (MQ=255) aaCCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTGGCGGAttatta > 1:2170550/1‑66 (MQ=255) aaCCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTGGCGGAttatta > 1:2328506/1‑66 (MQ=255) aaCCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTGGCGGAttatta > 1:902343/1‑66 (MQ=255) aaCCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTGGCGGAttatta > 1:2598963/1‑66 (MQ=255) aaCCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTGGCGGAttatta > 1:1000300/1‑66 (MQ=255) aaCCACAGAACGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTGGCGGAttatta > 1:1832415/1‑66 (MQ=255) cGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGc < 1:165601/69‑1 (MQ=255) cGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGc < 1:967281/69‑1 (MQ=255) cGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGc < 1:1050998/69‑1 (MQ=255) cGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGc < 1:2936238/69‑1 (MQ=255) cGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGc < 1:2382818/69‑1 (MQ=255) cGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGc < 1:1130180/69‑1 (MQ=255) cGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGc < 1:1374584/69‑1 (MQ=255) gACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGcc < 1:2002373/69‑1 (MQ=255) gACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGc < 1:1581502/68‑1 (MQ=255) gACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGc < 1:2750455/68‑1 (MQ=255) tGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCAtt < 1:2702799/69‑1 (MQ=255) tGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCAtt < 1:2747527/69‑1 (MQ=255) tGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCAtt < 1:1074661/69‑1 (MQ=255) tGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCAtt < 1:3251536/69‑1 (MQ=255) tGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCAtt < 1:3260390/69‑1 (MQ=255) tGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCAtt < 1:2242298/69‑1 (MQ=255) tGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCAtt < 1:1133504/69‑1 (MQ=255) tGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCAtt < 1:1275943/69‑1 (MQ=255) tGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCAtt < 1:832058/69‑1 (MQ=255) tGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCAtt < 1:533887/69‑1 (MQ=255) tGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCAtt < 1:1392044/69‑1 (MQ=255) tGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCAtt < 1:736822/69‑1 (MQ=255) tGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCAtt < 1:790379/69‑1 (MQ=255) tGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCAtt < 1:1481948/69‑1 (MQ=255) tGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCAtt < 1:1770362/69‑1 (MQ=255) ggTCGTGCGTCTGGTGCATGATTGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCAttt > 1:1720152/1‑69 (MQ=255) ggTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCAttt > 1:3361335/1‑69 (MQ=255) ggTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCAttt > 1:2972840/1‑69 (MQ=255) ggTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCAttt > 1:811753/1‑69 (MQ=255) ggTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCAttt > 1:1003775/1‑69 (MQ=255) ggTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCAttt > 1:2107607/1‑69 (MQ=255) ggTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCAtt < 1:836466/68‑1 (MQ=255) cGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTcc < 1:1013070/68‑1 (MQ=255) cGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTcc < 1:961932/68‑1 (MQ=255) cGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTcc < 1:598254/68‑1 (MQ=255) cGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTcc < 1:2823110/68‑1 (MQ=255) cGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTcc < 1:2464956/68‑1 (MQ=255) cGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTcc < 1:2342680/68‑1 (MQ=255) cGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTcc < 1:181543/68‑1 (MQ=255) cGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTcc < 1:6287/68‑1 (MQ=255) cGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTcc < 1:1991802/68‑1 (MQ=255) gTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTcc < 1:540619/67‑1 (MQ=255) gTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTcc < 1:2291963/67‑1 (MQ=255) tCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGc < 1:32846/68‑1 (MQ=255) tCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGc < 1:2694121/68‑1 (MQ=255) tCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGc < 1:3401580/68‑1 (MQ=255) tCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGc < 1:779870/68‑1 (MQ=255) tCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGc < 1:851450/68‑1 (MQ=255) tCTGGTGCATGATCGTGATGCCTGGCGTCTGGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGcc < 1:195479/69‑1 (MQ=255) tCTGGTGCATGATCGTGATGCCTGGCGTCTGGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGcc < 1:646181/69‑1 (MQ=255) tCTGGTGCATGATCGTGATGCCTGGCGTCTGGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGcc < 1:528194/69‑1 (MQ=255) tCTGGTGCATGATCGTGATGCCTGGCGTCTGGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGcc < 1:878746/69‑1 (MQ=255) tCTGGTGCATGATCGTGATGCCTGGCGTCTGGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGcc < 1:973843/69‑1 (MQ=255) tCTGGTGCATGATCGTGATGCCTGGCGTCTGGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGcc < 1:3397522/69‑1 (MQ=255) tCTGGTGCATGATCGTGATGCCTGGCGTCTGGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGcc < 1:2253596/69‑1 (MQ=255) tCTGGTGCATGATCGTGATGCCTGGCGTCTGGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGcc < 1:331420/69‑1 (MQ=255) tCTGGTGCATGATCGTGATGCCTGGCGTCTGGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGcc < 1:258982/69‑1 (MQ=255) tCTGGTGCATGATCGTGATGCCTGGCGTCTGGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGcc < 1:3045437/69‑1 (MQ=255) tCTGGTGCATGATCGTGATGCCTGGCGTCTGGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGcc < 1:3049316/69‑1 (MQ=255) tCTGGTGCATGATCGTGATGCCTGGCGTCTGGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGcc < 1:3127597/69‑1 (MQ=255) cTGGTGCATGATCGTGATGCCTGGCGTCTGGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGcc < 1:2109569/68‑1 (MQ=255) ggTGCATGATCGTGATGCCTGGCGTCTGGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGcc > 1:3276483/1‑66 (MQ=255) ggTGCATGATCGTGATGCCTGGCGTCTGGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCt > 1:3132255/1‑68 (MQ=255) ggTGCATGATCGTGATGCCTGGCGTCTGGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCt > 1:3060480/1‑68 (MQ=255) ggTGCATGATCGTGATGCCTGGCGTCTGGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCt > 1:3236646/1‑68 (MQ=255) ggTGCATGATCGTGATGCCTGGCGTCTGGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCt > 1:1469331/1‑68 (MQ=255) ggTGCATGATCGTGATGCCTGGCGTCTGGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCt > 1:1261233/1‑68 (MQ=255) tGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTggg > 1:2714720/1‑69 (MQ=255) tGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTggg > 1:1071570/1‑69 (MQ=255) tGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTggg > 1:3173836/1‑69 (MQ=255) cATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGc < 1:57302/61‑1 (MQ=255) ccTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCAtt < 1:884142/39‑1 (MQ=255) gTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGcc < 1:132834/69‑1 (MQ=255) gTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGcc < 1:2619729/69‑1 (MQ=255) gTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGcc < 1:2572972/69‑1 (MQ=255) gTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGcc < 1:2531058/69‑1 (MQ=255) gTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGcc < 1:2324844/69‑1 (MQ=255) gTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGcc < 1:1425373/69‑1 (MQ=255) gTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGcc < 1:803030/69‑1 (MQ=255) gTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGcc < 1:742367/69‑1 (MQ=255) gTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGcc < 1:1842081/69‑1 (MQ=255) gTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGcc < 1:627694/69‑1 (MQ=255) gTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGc < 1:88577/68‑1 (MQ=255) | TGCGCTTAACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCC > minE/716421‑716546 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |