| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 72,887 | T→A | 55.0% | A40A (GCT→GCA) | fruR → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 72,887 | 0 | T | A | 55.0% | 26.2 / 97.9 | 91 | A40A (GCT→GCA) | fruR | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (5/36); new base A (22/28); total (27/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.13e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
Only 100 of 111 total aligned reads displayed.
AGCTATGTTATTAACGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTC > minE/72819‑72955 | aGCTATGTTATTAACGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCt > 1:555739/1‑69 (MQ=255)aGCTATGTTATTAACGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCt > 1:2151417/1‑69 (MQ=255) cTATGTTATTAACGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCtgt < 1:1921391/69‑1 (MQ=255) ttattaACGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCtgt > 1:722805/1‑64 (MQ=255) ttattaACGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCtgt > 1:1752802/1‑64 (MQ=255) ttattaACGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCtgt > 1:2438354/1‑64 (MQ=255) ttattaACGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCtgt > 1:2697486/1‑64 (MQ=255) ttattaACGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCtgt > 1:1345735/1‑64 (MQ=255) taACGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTg < 1:823358/68‑1 (MQ=255) taACGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTg < 1:1960470/68‑1 (MQ=255) aCGGCAAAGCGAATCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc < 1:1584936/63‑1 (MQ=255) aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc < 1:1926769/63‑1 (MQ=255) aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc < 1:1998690/63‑1 (MQ=255) aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc < 1:3204299/63‑1 (MQ=255) aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc < 1:2191331/63‑1 (MQ=255) aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc < 1:220726/63‑1 (MQ=255) aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc < 1:2217315/63‑1 (MQ=255) aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc < 1:3176800/63‑1 (MQ=255) aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc < 1:1019601/63‑1 (MQ=255) aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc < 1:3317514/63‑1 (MQ=255) aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc < 1:36521/63‑1 (MQ=255) aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc < 1:36734/63‑1 (MQ=255) aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc < 1:606968/63‑1 (MQ=255) aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc < 1:628943/63‑1 (MQ=255) aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc < 1:647525/63‑1 (MQ=255) aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc < 1:674545/63‑1 (MQ=255) aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc < 1:945056/63‑1 (MQ=255) aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc < 1:947274/63‑1 (MQ=255) aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc < 1:986035/63‑1 (MQ=255) aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc < 1:1298562/63‑1 (MQ=255) aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc < 1:1334526/63‑1 (MQ=255) aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc < 1:1500666/63‑1 (MQ=255) aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc < 1:1097926/63‑1 (MQ=255) aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc < 1:1196990/63‑1 (MQ=255) aCGGCAAAGCGAAGCAATACCGTGTGAGAGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc < 1:3053710/63‑1 (MQ=255) ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac < 1:1382077/69‑1 (MQ=255) ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac < 1:2332229/69‑1 (MQ=255) ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac < 1:2390470/69‑1 (MQ=255) ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac < 1:1466619/69‑1 (MQ=255) ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac < 1:2473071/69‑1 (MQ=255) ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac < 1:2561619/69‑1 (MQ=255) ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac < 1:2564327/69‑1 (MQ=255) ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac < 1:1194394/69‑1 (MQ=255) ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac < 1:2991426/69‑1 (MQ=255) ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac < 1:3037492/69‑1 (MQ=255) ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac < 1:328232/69‑1 (MQ=255) ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac < 1:1030288/69‑1 (MQ=255) ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac < 1:1972617/69‑1 (MQ=255) ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac < 1:203907/69‑1 (MQ=255) ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac < 1:2135678/69‑1 (MQ=255) ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac < 1:1645373/69‑1 (MQ=255) gAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCtgt < 1:2154041/48‑1 (MQ=255) aaGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTaccac < 1:2959075/69‑1 (MQ=255) aaGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTaccac < 1:1571298/69‑1 (MQ=255) aaGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTaccac < 1:1268749/69‑1 (MQ=255) aaGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTaccac < 1:247128/69‑1 (MQ=255) aaTACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTg < 1:2344855/51‑1 (MQ=255) aCCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCtt > 1:2151115/1‑69 (MQ=255) aCCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCtt > 1:2607574/1‑69 (MQ=255) aCCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCtt > 1:2809741/1‑69 (MQ=255) aCCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTCGGCtt > 1:2513851/1‑69 (MQ=255) cGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCg < 1:858507/69‑1 (MQ=255) cGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCg < 1:3415512/69‑1 (MQ=255) aaaaaGTCATGGCTGTGGTGCGTGAGCACAATTaccac < 1:1188957/38‑1 (MQ=255) aaaaGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTg < 1:1677127/68‑1 (MQ=255) aaaaGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTg < 1:59287/68‑1 (MQ=255) aaaaGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTg < 1:1961008/68‑1 (MQ=255) aaaaGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTg < 1:1975233/68‑1 (MQ=255) aaaaGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTg < 1:3079646/68‑1 (MQ=255) aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca > 1:1103738/1‑69 (MQ=255) aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca > 1:695045/1‑69 (MQ=255) aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca > 1:111348/1‑69 (MQ=255) aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca > 1:363663/1‑69 (MQ=255) aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca > 1:1939772/1‑69 (MQ=255) aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca > 1:391704/1‑69 (MQ=255) aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca > 1:3215176/1‑69 (MQ=255) aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca > 1:3013700/1‑69 (MQ=255) aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca > 1:262155/1‑69 (MQ=255) aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca > 1:1525457/1‑69 (MQ=255) aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca > 1:2563546/1‑69 (MQ=255) aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca > 1:1671477/1‑69 (MQ=255) aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca > 1:2519682/1‑69 (MQ=255) aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca > 1:1651731/1‑69 (MQ=255) aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca > 1:2089175/1‑69 (MQ=255) aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca > 1:2443621/1‑69 (MQ=255) aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca > 1:2439491/1‑69 (MQ=255) aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca > 1:2439371/1‑69 (MQ=255) aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcac > 1:2229251/1‑68 (MQ=255) aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcac > 1:1285546/1‑68 (MQ=255) aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGACGTGGCAGCTGGGCTTCGTGCTGGACGcaca > 1:1222863/1‑69 (MQ=255) tgtgGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTc > 1:754191/1‑69 (MQ=255) tgtgGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTc > 1:1105271/1‑69 (MQ=255) tgtgGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTc > 1:1011648/1‑69 (MQ=255) tgtgGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTc > 1:693739/1‑69 (MQ=255) tgtgGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTc < 1:2382105/69‑1 (MQ=255) tgtgGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTc < 1:64798/69‑1 (MQ=255) tgtgGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTc > 1:2389089/1‑69 (MQ=255) tgtgGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTc < 1:401011/69‑1 (MQ=255) tgtgGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTc > 1:379509/1‑69 (MQ=255) tgtgGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTc < 1:1298288/69‑1 (MQ=255) | AGCTATGTTATTAACGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTC > minE/72819‑72955 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |