| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 720,741 | A→G | 42.5% | V961A (GTC→GCC) | rne ← | fused ribonucleaseE endoribonuclease and scaffold for formation of degradosome |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 720,741 | 0 | A | G | 42.5% | 18.2 / 50.9 | 69 | V961A (GTC→GCC) | rne | fused ribonucleaseE endoribonuclease and scaffold for formation of degradosome |
| Reads supporting (aligned to +/- strand): ref base A (7/32); new base G (17/12); total (24/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.80e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.97e-01 | |||||||||||
CGACTACTGGCGCGGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCTGCTTGTTCTGCAACT > minE/720676‑720806 | cGACTACTGGCGCGGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACa < 1:202668/68‑1 (MQ=255)cGACTACTGGCGCGGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACa < 1:816411/68‑1 (MQ=255)cGACTACTGGCGCGGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACa < 1:1874826/68‑1 (MQ=255)cGACTACTGGCGCGGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACa < 1:2382218/68‑1 (MQ=255) gcgGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCTACAACTTCTTCAat < 1:1539985/68‑1 (MQ=255) gcgGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGCCAACTTCTTCAat < 1:162527/68‑1 (MQ=255) gcgGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAat < 1:100355/68‑1 (MQ=255) gcgGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAat < 1:508703/68‑1 (MQ=255) gcgGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAat < 1:2207445/68‑1 (MQ=255) gcgGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAat < 1:2977031/68‑1 (MQ=255) gcgGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAat < 1:1839677/68‑1 (MQ=255) gcgGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAat < 1:2272585/68‑1 (MQ=255) gcgGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAat < 1:2296945/68‑1 (MQ=255) gcgGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAat < 1:2315060/68‑1 (MQ=255) gcgGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAat < 1:79463/68‑1 (MQ=255) cgGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAat < 1:1369979/67‑1 (MQ=255) aacTTCAGGTTCAGCAACTTCAACCTCAGCAGTTTCGACATCTTCTTCAat < 1:1537038/51‑1 (MQ=255) cAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAActtc > 1:3160881/1‑40 (MQ=255) cAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCgg > 1:908073/1‑67 (MQ=255) cAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGt > 1:737882/1‑68 (MQ=255) cAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGt > 1:1193390/1‑68 (MQ=255) ggTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAat < 1:2052594/44‑1 (MQ=255) ggTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTc < 1:621707/68‑1 (MQ=255) ggTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTc < 1:1351700/68‑1 (MQ=255) ggTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTc < 1:3083223/68‑1 (MQ=255) ggTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTc < 1:3346841/68‑1 (MQ=255) gTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTc < 1:2745595/67‑1 (MQ=255) cAACCACAACCTCAGCAGTTTCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCTCCg < 1:2162500/69‑1 (MQ=255) cAACCACAACCTCAGCAGTTTCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCg < 1:3239556/69‑1 (MQ=255) cAACCACAACCTCAGCAGTTTCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCg < 1:207003/69‑1 (MQ=255) cAACCACAACCTCAGCAGTTTCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCg < 1:1959192/69‑1 (MQ=255) cAACCACAACCTCAGCAGTTTCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCg < 1:566674/69‑1 (MQ=255) cAACCACAACCTCAGCAGTTTCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCg < 1:2252695/69‑1 (MQ=255) cAACCACAACCTCAGCAGTTTCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCg < 1:1791205/69‑1 (MQ=255) cAACCACAACCTCAGCAGTTTCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCg < 1:1767009/69‑1 (MQ=255) cAACCACAACCTCAGCAGTTTCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCg < 1:716435/69‑1 (MQ=255) cAACCACAACCTCAGCAGTTTCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCg < 1:280935/69‑1 (MQ=255) cAACCACAACCTCAGCAGTTTCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCg < 1:2800564/69‑1 (MQ=255) aaCTACAACCTCAGCTGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCgg < 1:1964885/69‑1 (MQ=255) aaCTACAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCgg < 1:739118/69‑1 (MQ=255) aaCTACAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCgg < 1:2394705/69‑1 (MQ=255) aaCTACAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCgg < 1:2756832/69‑1 (MQ=255) aaCTACAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCAACgg < 1:942005/69‑1 (MQ=255) aCAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:1204328/1‑69 (MQ=255) aCAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:2012560/1‑69 (MQ=255) cTCAGCAGTTTCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCg < 1:1982305/59‑1 (MQ=255) tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCACCCACCGGGTCt > 1:2215482/1‑54 (MQ=255) tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:400494/1‑54 (MQ=255) tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:1118124/1‑54 (MQ=255) tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:896367/1‑54 (MQ=255) tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:1205229/1‑54 (MQ=255) tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:1229419/1‑54 (MQ=255) tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:1430470/1‑54 (MQ=255) tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:1476721/1‑54 (MQ=255) tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:1513170/1‑54 (MQ=255) tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:1905131/1‑54 (MQ=255) tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:1968394/1‑54 (MQ=255) tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:39724/1‑54 (MQ=255) tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:3119507/1‑54 (MQ=255) tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:2220511/1‑54 (MQ=255) tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:2814148/1‑54 (MQ=255) tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:2337941/1‑54 (MQ=255) tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:2791673/1‑54 (MQ=255) tCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCTGCTTGTTCTGCa < 1:2639344/66‑1 (MQ=255) tCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCTGCTTGTTCTGCa < 1:563586/66‑1 (MQ=255) tCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCTGCTTGTTCTGCa < 1:1589694/66‑1 (MQ=255) tCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCTGCTTGTTCTGCa < 1:2014107/66‑1 (MQ=255) tCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCTGCTTGTTCTGCa < 1:2631808/66‑1 (MQ=255) tCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCTGCTTGTTCTGCAACt > 1:392383/1‑69 (MQ=255) | CGACTACTGGCGCGGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCTGCTTGTTCTGCAACT > minE/720676‑720806 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |