| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 728,538 | T→C | 38.8% | I175I (ATT→ATC) | fabH → | 3‑oxoacyl‑[acyl‑carrier‑protein] synthase III |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 728,538 | 0 | T | C | 38.8% | 23.5 / 34.0 | 54 | I175I (ATT→ATC) | fabH | 3‑oxoacyl‑[acyl‑carrier‑protein] synthase III |
| Reads supporting (aligned to +/- strand): ref base T (23/10); new base C (12/9); total (35/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.58e-01 | |||||||||||
TTATTATTTTTGGCGATGGCGCGGGCGCTGCGGTGCTGGCTGCCTCTGAAGAGCCGGGAATCATTTCCACCCATCTGCATGCCGACGGTAGTTATGGTGAATTGCTGACGCTGCCAAACGCCGACCGC > minE/728474‑728601 | ttattattTTTGGCGATGGCGCGGGCGCTGCGGTGCTGGCTGCCTCTGAAGAGCCGGGAATCATTTcc > 1:742991/1‑68 (MQ=255)ttattattTTTGGCGATGGCGCGGGCGCTGCGGTGCTGGCTGCCTCTGAAGAGCCGGGAATCATTTCCa > 1:2166463/1‑69 (MQ=255)ttattattTTTGGCGATGGCGCGGGCGCTGCGGTGCTGGCTGCCTCTGAAGAGCCGGGAATCATTTCCa > 1:556565/1‑69 (MQ=255)ttattattTTTGGCGATGGCGCGGGCGCTGCGGTGCTGGCTGCCTCTGAAGAGCCGGGAATCATTTCCa > 1:2649329/1‑69 (MQ=255)ttattattTTTGGCGATGGCGCGGGCGCTGCGGTGCTGGCTGCCTCTGAAGAGCCGGGAATCATTTCCa > 1:758510/1‑69 (MQ=255)ttattattTTTGGCGATGGCGCGGGCGCTGCGGTGCTGGCTGCCTCTGAAGAGCCGGGAATCATTTCCa > 1:1432661/1‑69 (MQ=255)ttattattTTTGGCGATGGCGCGGGCGCTGCGGTGCTGGCTGCCTCTGAAGAGCCGGGAATCATTTCCa > 1:1459274/1‑69 (MQ=255) tttGGCGATGGCGCGGGCGCTGCGGTGCTGGCTGCCTCTGAAGAGCCGGGAATCATTTCCACCCATCTg < 1:864884/69‑1 (MQ=255) tttGGCGATGGCGCGGGCGCTGCGGTGCTGGCTGCCTCTGAAGAGCCGGGAATCATTTCCACCCATCTg < 1:2570620/69‑1 (MQ=255) tttGGCGATGGCGCGGGCGCTGCGGTGCTGGCTGCCTCTGAAGAGCCGGGAATCATTTCCACCCATCTg < 1:1754566/69‑1 (MQ=255) gcgcGGGCGCTGCGGTGCTGGCTGCCTCTGAAGAGCCGGGAATCATTTc < 1:817722/49‑1 (MQ=255) gcgcGGGCGCTGCGGTGCTGGCTGCCTCTGAAGAGCCGGGAATCATTTc < 1:125549/49‑1 (MQ=255) ggCGCTGCGGTGCTGGCTGCCTCTGAAGAGCCGGGAATCATTTCCACCCATCTGCATGCCGACGGTAGt > 1:409819/1‑69 (MQ=255) ggCGCTGCGGTGCTGGCTGCCTCTGAAGAGCCGGGAATCATTTCCACCCATCTGCATGCCGACGGTAGt > 1:1358730/1‑69 (MQ=255) ggCGCTGCGGTGCTGGCTGCCTCTGAAGAGCCGGGAATCATTTCCACCCATCTGCATGCCGACGGTAGt > 1:719249/1‑69 (MQ=255) ggCGCTGCGGTGCTGGCTGCCTCTGAAGAGCCGGGAATCATTTCCACCCATCTGCATGCCGACGGTAGt > 1:2782660/1‑69 (MQ=255) ggCGCTGCGGTGCTGGCTGCCTCTGAAGAGCCGGGAATCATTTCCACCCATCTGCATGCCGACGGTAGt < 1:1845060/69‑1 (MQ=255) ggCGCTGCGGTGCTGGCTGCCTCTGAAGAGCCGGGAATCATTTCCACCCATCTGCATGCCGACGGTAGt < 1:854847/69‑1 (MQ=255) ggCGCTGCGGTGCTGGCTGCCTCTGAAGAGCCGGGAATCATTTCCACCCATCTGCATGCCGACGGTAGt > 1:1947116/1‑69 (MQ=255) ggCGCTGCGGTGCTGGCTGCCTCTGAAGAGCCGGGAATCATTTCCACCCATCTGCATGCCGACGGTAGt > 1:3237160/1‑69 (MQ=255) ggCGCTGCGGTGCTGGCTGCCTCTGAAGAGCCGGGAATCATTTCCACCCATCTGCATGCCGACGGTAGt > 1:3074882/1‑69 (MQ=255) gTGCTGGCTGCCTCTGAAGAGCCGGGAATCATCTCCACCCATCTGCATGCCGACGGTAGCTATGGTGa < 1:3037171/68‑1 (MQ=255) gTGCTGGCTGCCTCTGAAGAGCCGGGAATCATCTCCACCCATCTGCATGCCGACGGTAGCTATGGTGa < 1:2464329/68‑1 (MQ=255) gTGCTGGCTGCCTCTGAAGAGCCGGGAATCATCTCCACCCATCTGCATGCCGACGGTAGCTATGGTGa < 1:636226/68‑1 (MQ=255) gTGCTGGCTGCCTCTGAAGAGCCGGGAATCATCTCCACCCATCTGCATGCCGACGGTAGCTATGGTGa < 1:681261/68‑1 (MQ=255) gTGCTGGCTGCCTCTGAAGAGCCGGGAATCATCTCCACCCATCTGCATGCCGACGGTAGCTATGGTGa < 1:69192/68‑1 (MQ=255) gTGCTGGCTGCCTCTGAAGAGCCGGGAATCATCTCCACCCATCTGCATGCCGACGGTAGCTATGGTGa < 1:1395079/68‑1 (MQ=255) gTGCTGGCTGCCTCTGAAGAGCCGGGAATCATCTCCACCCATCTGCATGCCGACGGTAGCTATGGTGa < 1:831251/68‑1 (MQ=255) gTGCTGGCTGCCTCTGAAGAGCCGGGAATCATCTCCACCCATCTGCATGCCGACGGTAGCTATGGTGa < 1:1083011/68‑1 (MQ=255) gTGCTGGCTGCCTCTGAAGAGCCGGGAATCATCTCCACCCATCTGCATGCCGACGGTAGCTATGGTGa < 1:972348/68‑1 (MQ=255) gctggctgCCTCTGAAGAGCCGGGAATCATTTCCACCCATCTGCATGCCGACGGTAGTTATGGTg < 1:106420/65‑1 (MQ=255) gctggctgCCTCTGAAGAGCCGGGAATCATTTCCACCCATCTGCATGCCGACGGTAGTTATGGTg < 1:1933741/65‑1 (MQ=255) gctggctgCCTCTGAAGAGCCGGGAATCATTTCCACCCATCTGCATGCCGACGGTAGTTATGGTg < 1:1937794/65‑1 (MQ=255) ctggctgCCTCTGAAGAGCCGGGAATCATCTCCACCCATCTGCATGCCGACgg > 1:984112/1‑53 (MQ=255) ctggctgCCTCTGAAGAGCCGGGAATCATCTCCACCCATCTGCATGCCGACgg > 1:1163992/1‑53 (MQ=255) ctggctgCCTCTGAAGAGCCGGGAATCATCTCCACCCATCTGCATGCCGACgg > 1:1169042/1‑53 (MQ=255) ctggctgCCTCTGAAGAGCCGGGAATCATCTCCACCCATCTGCATGCCGACgg > 1:1675348/1‑53 (MQ=255) ctggctgCCTCTGAAGAGCCGGGAATCATCTCCACCCATCTGCATGCCGACgg > 1:908999/1‑53 (MQ=255) ctggctgCCTCTGAAGAGCCGGGAATCATCTCCACCCATCTGCATGCCGACgg > 1:2510334/1‑53 (MQ=255) ctggctgCCTCTGAAGAGCCGGGAATCATCTCCACCCATCTGCATGCCGACgg > 1:3404366/1‑53 (MQ=255) ctggctgCCTCTGAAGAGCCGGGAATCATCTCCACCCATCTGCATGCCGACgg > 1:3374742/1‑53 (MQ=255) ctggctgCCTCTGAAGAGCCGGGAATCATCTCCACCCATCTGCATGCCGACgg > 1:2797590/1‑53 (MQ=255) ctggctgCCTCTGAAGAGCCGGGAATCATCTCCACCCATCTGCATGCCGACgg > 1:3118973/1‑53 (MQ=255) ctggctgCCTCTGAAGAGCCGGGAATCATCTCCACCCATCTGCATGCCGACg > 1:1319930/1‑52 (MQ=255) ctggctgCCTCTGAAGAGCCGGGAATCATCTCCACCCATCTGCAAGCCGACg > 1:890712/1‑52 (MQ=255) gAAGAGCCGGGAATCATTTCCACCCATCTGCATGCCGACGGTAGTTATGGTGAATTGCt > 1:423717/1‑59 (MQ=255) gAAGAGCCGGGAATCATTTCCACCCATCTGCATGCCGACGGTAGTTATGGTGAATTGCt > 1:2668413/1‑59 (MQ=255) gAAGAGCCGGGAATCATTTCCACCCATCTGCATGCCGACGGTAGTTATGGTGAATTGCt > 1:1687263/1‑59 (MQ=255) gAAGAGCCGGGAATCATTTCCACCCATCTGCATGCCGACGGTAGTTATGGTGAATTGCt > 1:2958788/1‑59 (MQ=255) gCCGGGAATCATTTCCACCCATCTGCATGCCGACGGTAGTTATGGTGAATTGCTGACGCTGCCAAACg > 1:2931415/1‑68 (MQ=255) atcatcTCCACCCATCTGCATGCCGACGGTAGCTATGGTGa < 1:9380/41‑1 (MQ=255) aTCATTTCCACCCATCTGCATGCCGACGGTAGTTATGGTGAATTGCTGACGCTGCCAAACGCCGACcgc > 1:1601462/1‑69 (MQ=255) aTCATTTCCACCCATCTGCATGCCGACGGTAGTTATGGTGAATTGCTGACGCTGCCAAACGCCGACcgc > 1:1708350/1‑69 (MQ=255) aTCATTTCCACCCATCTGCATGCCGACGGTAGTTATGGTGAATTGCTGACGCTGCCAAACGCCGACcgc > 1:1022597/1‑69 (MQ=255) aTCATTTCCACCCATCTGCATGCCGACGGTAGTTATGGTGAATTGCTGACGCTGCCAAACGCCGACcgc > 1:201752/1‑69 (MQ=255) | TTATTATTTTTGGCGATGGCGCGGGCGCTGCGGTGCTGGCTGCCTCTGAAGAGCCGGGAATCATTTCCACCCATCTGCATGCCGACGGTAGTTATGGTGAATTGCTGACGCTGCCAAACGCCGACCGC > minE/728474‑728601 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |