Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 728,796 T→G 27.4% S261S (TCT→TCG fabH → 3‑oxoacyl‑[acyl‑carrier‑protein] synthase III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE728,7960TG27.4% 105.2 / 53.4 91S261S (TCT→TCGfabH3‑oxoacyl‑[acyl‑carrier‑protein] synthase III
Reads supporting (aligned to +/- strand):  ref base T (31/35);  new base G (18/7);  total (49/42)
Fisher's exact test for biased strand distribution p-value = 3.70e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCG  >  minE/728735‑728862
                                                             |                                                                  
tggTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGAt                                                              <  1:3133094/68‑1 (MQ=255)
tggTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGAt                                                              <  1:2730181/68‑1 (MQ=255)
tggTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGAt                                                              <  1:307038/68‑1 (MQ=255)
        cATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCgt                                                      <  1:65188/68‑1 (MQ=255)
                 aaCCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATa                                                             >  1:133811/1‑52 (MQ=255)
                 aaCCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCgtggtgcc                                                >  1:1290415/1‑63 (MQ=255)
                 aaCCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTTGTGAc                                                >  1:1219238/1‑65 (MQ=255)
                 aaCCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGAc                                                >  1:902677/1‑65 (MQ=255)
                 aaCCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGAc                                                >  1:950128/1‑65 (MQ=255)
                 aaCCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGAc                                                >  1:1921928/1‑65 (MQ=255)
                 aaCCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGAc                                                >  1:300978/1‑65 (MQ=255)
                 aaCCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGAc                                                >  1:3075857/1‑65 (MQ=255)
                 aaCCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGAc                                                >  1:3097888/1‑65 (MQ=255)
                 aaCCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGAc                                                >  1:321110/1‑65 (MQ=255)
                    cTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCTTGGTGACGCTgg                                           <  1:1893595/67‑1 (MQ=255)
                    cTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTgg                                           <  1:3086387/67‑1 (MQ=255)
                    cTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTgg                                           <  1:3082325/67‑1 (MQ=255)
                    cTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTgg                                           <  1:1060854/67‑1 (MQ=255)
                    cTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTgg                                           <  1:902953/67‑1 (MQ=255)
                    cTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTgg                                           <  1:2931840/67‑1 (MQ=255)
                    cTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTgg                                           <  1:3302519/67‑1 (MQ=255)
                    cTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTgg                                           <  1:2768262/67‑1 (MQ=255)
                    cTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTgg                                           <  1:466880/67‑1 (MQ=255)
                    cTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTgg                                           <  1:1154931/67‑1 (MQ=255)
                    cTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTgg                                           <  1:1435035/67‑1 (MQ=255)
                    cTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTgg                                           <  1:1240042/67‑1 (MQ=255)
                    cTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTgg                                           <  1:1053448/67‑1 (MQ=255)
                     tGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGCCGCTGGATc                                        >  1:2299305/1‑69 (MQ=255)
                     tGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATc                                        >  1:3433075/1‑69 (MQ=255)
                     tGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATc                                        >  1:2511058/1‑69 (MQ=255)
                     tGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATc                                        >  1:3252856/1‑69 (MQ=255)
                     tGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATc                                        >  1:1281828/1‑69 (MQ=255)
                     tGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATc                                        >  1:451776/1‑69 (MQ=255)
                     tGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATc                                        >  1:16297/1‑69 (MQ=255)
                     tGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATc                                        >  1:920628/1‑69 (MQ=255)
                     tGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATc                                        >  1:2291995/1‑69 (MQ=255)
                     tGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATc                                        >  1:1361382/1‑69 (MQ=255)
                      gCGTATTATCAGTGCAACGGCGAAAAATCTCGGCATGTCGATGGACAATGTCGTGGTGAGGCTGGATc                                        >  1:846608/1‑68 (MQ=255)
                      gCGTATTATCAGTGCAACGGCGAAAAAACTCGGCATGTCGATGGACAATGTCgtg                                                     >  1:1628762/1‑55 (MQ=255)
                      gCGTATTATCAGTGCAACGGCGAAAAAACTCGGCATGTCGATGGACAATGTCGTGGTGACGCTGGATc                                        >  1:493273/1‑68 (MQ=255)
                      gCGTATTATCAGTGCAACGGCGAAAAAACTCGGCATGTCGATGGACAATGTCGTGGTGACGCTGGATc                                        >  1:1949864/1‑68 (MQ=255)
                      gCGTATTATCAGTGCAACGGCGAAAAAACTCGGCATGTCGATGGACAATGTCGTGGTGACGCTGGATc                                        >  1:2986424/1‑68 (MQ=255)
                      gCGTATTATCAGTGCAACGGCGAAAAAACTCGGCATGTCGATGGACAATGTCGTGGTGACGCTGGATc                                        >  1:154826/1‑68 (MQ=255)
                      gCGTATTATCAGTGCAACGGCGAAAAAACTCGGCATGTCGATGGACAATGTCGTGGTGACGCTGGATc                                        >  1:3396259/1‑68 (MQ=255)
                      gCGTATTATCAGTGCAACGGCGAAAAAACTCGGCATGTCGATGGACAATGTCGTGGTGACGCTGGATc                                        >  1:607447/1‑68 (MQ=255)
                      gCGTATTATCAGTGCAACGGCGAAAAAACTCGGCATGTCGATGGACAATGTCGTGGTGACGCTGGATc                                        >  1:2702093/1‑68 (MQ=255)
                      gCGTATTATCAGTGCAACGGCGAAAAAACTCGGCATGTCGATGGACAATGTCGTGGTGACGCTGGATc                                        >  1:403235/1‑68 (MQ=255)
                      gCGTATTATCAGTGCAACGGCGAAAAAACTCGGCATGTCGATGGACAATGTCGTGGTGACGCTGGATc                                        >  1:2497571/1‑68 (MQ=255)
                      gCGTATTATCAGTGCAACGGCGAAAAAACTCGGCATGTCGATGGACAATGTCGTGGTGACGCTGGATc                                        >  1:2457599/1‑68 (MQ=255)
                      gCGTATTATCAGTGCAACGGCGAAAAAACTCGGCATGTCGATGGACAATGTCGTGGTGACGCTGGATc                                        >  1:239314/1‑68 (MQ=255)
                      gCGTATTATCAGTGCAACGGCGAAAAAACTCGGCATGTCGATGGACAATGTCGTGGTGACGCTGGATc                                        >  1:2343562/1‑68 (MQ=255)
                      gCGTATTATCAGTGCAACGGCGAAAAAACTCGGCATGTCGATGGACAATGTCGTGGTGACGCTGGATc                                        >  1:2255778/1‑68 (MQ=255)
                      gCGTATTATCAGTGCAACGGCGAAAAAACTCGGCATGTCGATGGACAATGTCGTGGTGACGCTGGATc                                        >  1:2228802/1‑68 (MQ=255)
                      gCGTATTATCAGTGCAACGGCGAAAAAACTCGGCATGTCGATGGACAATGTCGTGGTGACGCTGGATc                                        >  1:2170620/1‑68 (MQ=255)
                      gCGTATTATCAGAGCAACGGCGAAAAAACTCGGCATGTCGATGGACAATGTCGTGGTGACGCTGGATc                                        >  1:2267327/1‑68 (MQ=255)
                         tattatCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGc                                      <  1:1276582/67‑1 (MQ=255)
                         tattatCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGc                                      <  1:739628/67‑1 (MQ=255)
                           ttatCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCAc                                   >  1:70631/1‑68 (MQ=255)
                           ttatCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCAc                                   >  1:497202/1‑68 (MQ=255)
                             atCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCgtggt                                                   <  1:3040196/50‑1 (MQ=255)
                             atCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCgtggt                                                   <  1:1724664/50‑1 (MQ=255)
                             atCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCgtggt                                                   <  1:1930661/50‑1 (MQ=255)
                             atCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCgtggt                                                   <  1:2570853/50‑1 (MQ=255)
                             atCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGt                                <  1:3377613/69‑1 (MQ=255)
                             atCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGt                                <  1:2681054/69‑1 (MQ=255)
                             atCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGt                                <  1:244095/69‑1 (MQ=255)
                             atCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGATGACGCTGGATCGCCACGGt                                <  1:1476030/69‑1 (MQ=255)
                                  tGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATAc                           <  1:2721878/69‑1 (MQ=255)
                                  tGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATAc                           <  1:996354/69‑1 (MQ=255)
                                    cAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACCCTGGATCGCCACGGTAATACct                         >  1:2720754/1‑69 (MQ=255)
                                              aaaaCTCTGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCctc               <  1:756828/69‑1 (MQ=255)
                                              aaaaCTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCctc               <  1:807278/69‑1 (MQ=255)
                                              aaaaCTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCctc               <  1:2135022/69‑1 (MQ=255)
                                              aaaaCTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCctc               <  1:382120/69‑1 (MQ=255)
                                              aaaaCTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCctc               <  1:453270/69‑1 (MQ=255)
                                              aaaaCTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCctc               <  1:2886679/69‑1 (MQ=255)
                                               aaaCTCGGCATGTCGATGGACAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCctct              <  1:644860/69‑1 (MQ=255)
                                               aaaCTCGGCATGTCGATGGACAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCctct              <  1:1272171/69‑1 (MQ=255)
                                               aaaCTCGGCATGTCGATGGACAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCctct              <  1:235146/69‑1 (MQ=255)
                                               aaaCTCGGCATGTCGATGGACAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCctct              <  1:725559/69‑1 (MQ=255)
                                               aaaCTCGGCATGTCGATGGACAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCctct              <  1:794852/69‑1 (MQ=255)
                                               aaaCTCGGCATGTCGATGGACAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCctct              <  1:3075654/69‑1 (MQ=255)
                                                    cGGCATGTCGATGGACAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCctct              <  1:3432524/64‑1 (MQ=255)
                                                           tCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTgcgcg  >  1:2132653/1‑69 (MQ=255)
                                                           tCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTgcgcg  >  1:2373343/1‑69 (MQ=255)
                                                           tCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTgcgcg  >  1:3022932/1‑69 (MQ=255)
                                                           tCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTgcgcg  >  1:3049372/1‑69 (MQ=255)
                                                           tCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTgcgcg  >  1:1502287/1‑69 (MQ=255)
                                                           tCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTgcgcg  >  1:3082412/1‑69 (MQ=255)
                                                           tCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTgcgcg  >  1:1392259/1‑69 (MQ=255)
                                                           tCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTgcgcg  >  1:516422/1‑69 (MQ=255)
                                                             |                                                                  
TGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCG  >  minE/728735‑728862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: