Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 72,890 G→A 54.9% V41V (GTG→GTA fruR → DNA‑binding transcriptional dual regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE72,8900GA54.9% 13.4 / 75.6 91V41V (GTG→GTAfruRDNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base G (5/36);  new base A (22/28);  total (27/64)
Fisher's exact test for biased strand distribution p-value = 1.13e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

TAACGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCT  >  minE/72830‑72956
                                                            |                                                                  
taACGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTg                                                             <  1:1960470/68‑1 (MQ=255)
taACGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTg                                                             <  1:823358/68‑1 (MQ=255)
  aCGGCAAAGCGAATCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc                                                                <  1:1584936/63‑1 (MQ=255)
  aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc                                                                <  1:36734/63‑1 (MQ=255)
  aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc                                                                <  1:1926769/63‑1 (MQ=255)
  aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc                                                                <  1:1998690/63‑1 (MQ=255)
  aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc                                                                <  1:2191331/63‑1 (MQ=255)
  aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc                                                                <  1:220726/63‑1 (MQ=255)
  aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc                                                                <  1:2217315/63‑1 (MQ=255)
  aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc                                                                <  1:3176800/63‑1 (MQ=255)
  aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc                                                                <  1:3204299/63‑1 (MQ=255)
  aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc                                                                <  1:3317514/63‑1 (MQ=255)
  aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc                                                                <  1:1019601/63‑1 (MQ=255)
  aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc                                                                <  1:391320/63‑1 (MQ=255)
  aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc                                                                <  1:606968/63‑1 (MQ=255)
  aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc                                                                <  1:628943/63‑1 (MQ=255)
  aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc                                                                <  1:647525/63‑1 (MQ=255)
  aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc                                                                <  1:674545/63‑1 (MQ=255)
  aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc                                                                <  1:680738/63‑1 (MQ=255)
  aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc                                                                <  1:945056/63‑1 (MQ=255)
  aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc                                                                <  1:947274/63‑1 (MQ=255)
  aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc                                                                <  1:986035/63‑1 (MQ=255)
  aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc                                                                <  1:1334526/63‑1 (MQ=255)
  aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc                                                                <  1:1298562/63‑1 (MQ=255)
  aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc                                                                <  1:1097926/63‑1 (MQ=255)
  aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc                                                                <  1:1196990/63‑1 (MQ=255)
  aCGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc                                                                <  1:1474311/63‑1 (MQ=255)
  aCGGCAAAGCGAAGCAATACCGTGTGAGAGACAAAACCGTTGAAAAAGTCATGGCAGTAGTGc                                                                <  1:3053710/63‑1 (MQ=255)
    ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac                                                        <  1:328232/69‑1 (MQ=255)
    ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac                                                        <  1:2561619/69‑1 (MQ=255)
    ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac                                                        <  1:2473071/69‑1 (MQ=255)
    ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac                                                        <  1:2696426/69‑1 (MQ=255)
    ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac                                                        <  1:1198203/69‑1 (MQ=255)
    ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac                                                        <  1:2390470/69‑1 (MQ=255)
    ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac                                                        <  1:2332229/69‑1 (MQ=255)
    ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac                                                        <  1:2991426/69‑1 (MQ=255)
    ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac                                                        <  1:1194394/69‑1 (MQ=255)
    ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac                                                        <  1:3037492/69‑1 (MQ=255)
    ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac                                                        <  1:1645373/69‑1 (MQ=255)
    ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac                                                        <  1:1382077/69‑1 (MQ=255)
    ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac                                                        <  1:1972617/69‑1 (MQ=255)
    ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac                                                        <  1:2135678/69‑1 (MQ=255)
    ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac                                                        <  1:203907/69‑1 (MQ=255)
    ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac                                                        <  1:1466619/69‑1 (MQ=255)
    ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGcac                                                        <  1:1030288/69‑1 (MQ=255)
    ggCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTAATGGCTGTGGTGCGTGAGcac                                                        <  1:3183353/69‑1 (MQ=255)
             aaGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTaccac                                               <  1:1571298/69‑1 (MQ=255)
             aaGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTaccac                                               <  1:2959075/69‑1 (MQ=255)
             aaGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTaccac                                               <  1:247128/69‑1 (MQ=255)
             aaGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTaccac                                               <  1:1268749/69‑1 (MQ=255)
                 aaTACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTg                                                             <  1:2344855/51‑1 (MQ=255)
                                     aCCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCtt                       >  1:2809741/1‑69 (MQ=255)
                                     aCCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCtt                       >  1:2607574/1‑69 (MQ=255)
                                     aCCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCtt                       >  1:2464497/1‑69 (MQ=255)
                                     aCCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTCGGCtt                       >  1:2513851/1‑69 (MQ=255)
                                       cGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCg                     <  1:858507/69‑1 (MQ=255)
                                       cGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCg                     <  1:3415512/69‑1 (MQ=255)
                                            aaaaaGTCATGGCTGTGGTGCGTGAGCACAATTaccac                                               <  1:1188957/38‑1 (MQ=255)
                                             aaaaGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTg                <  1:1677127/68‑1 (MQ=255)
                                             aaaaGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTg                <  1:188145/68‑1 (MQ=255)
                                             aaaaGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTg                <  1:3079646/68‑1 (MQ=255)
                                             aaaaGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTg                <  1:1961008/68‑1 (MQ=255)
                                             aaaaGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTg                <  1:59287/68‑1 (MQ=255)
                                             aaaaGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTg                <  1:1975233/68‑1 (MQ=255)
                                                    aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca        >  1:262155/1‑69 (MQ=255)
                                                    aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca        >  1:3215176/1‑69 (MQ=255)
                                                    aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca        >  1:1103738/1‑69 (MQ=255)
                                                    aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca        >  1:695045/1‑69 (MQ=255)
                                                    aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca        >  1:363663/1‑69 (MQ=255)
                                                    aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca        >  1:391704/1‑69 (MQ=255)
                                                    aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca        >  1:111348/1‑69 (MQ=255)
                                                    aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca        >  1:1525457/1‑69 (MQ=255)
                                                    aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca        >  1:2065088/1‑69 (MQ=255)
                                                    aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca        >  1:2089175/1‑69 (MQ=255)
                                                    aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca        >  1:3013700/1‑69 (MQ=255)
                                                    aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca        >  1:1939772/1‑69 (MQ=255)
                                                    aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca        >  1:2439371/1‑69 (MQ=255)
                                                    aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca        >  1:2439491/1‑69 (MQ=255)
                                                    aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca        >  1:2443621/1‑69 (MQ=255)
                                                    aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca        >  1:2519682/1‑69 (MQ=255)
                                                    aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca        >  1:2563546/1‑69 (MQ=255)
                                                    aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca        >  1:1671477/1‑69 (MQ=255)
                                                    aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcaca        >  1:1651731/1‑69 (MQ=255)
                                                    aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcac         >  1:1285546/1‑68 (MQ=255)
                                                    aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGcac         >  1:2229251/1‑68 (MQ=255)
                                                    aTGGCAGTAGTGCGTGAGCACAATTATCACCCGAACGACGTGGCAGCTGGGCTTCGTGCTGGACGcaca        >  1:1222863/1‑69 (MQ=255)
                                                         tgtgGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTc   <  1:2382105/69‑1 (MQ=255)
                                                         tgtgGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTc   >  1:754191/1‑69 (MQ=255)
                                                         tgtgGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTc   >  1:693739/1‑69 (MQ=255)
                                                         tgtgGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTc   >  1:1105271/1‑69 (MQ=255)
                                                         tgtgGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTc   >  1:1011648/1‑69 (MQ=255)
                                                         tgtgGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTc   <  1:64798/69‑1 (MQ=255)
                                                         tgtgGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTc   <  1:401011/69‑1 (MQ=255)
                                                         tgtgGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTc   >  1:2389089/1‑69 (MQ=255)
                                                         tgtgGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTc   >  1:379509/1‑69 (MQ=255)
                                                         tgtgGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTc   <  1:1298288/69‑1 (MQ=255)
                                                          gtggtgCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCt  >  1:2273862/1‑69 (MQ=255)
                                                          gtggtgCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCt  >  1:1814469/1‑69 (MQ=255)
                                                          gtggtgCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCt  >  1:2780739/1‑69 (MQ=255)
                                                          gtggtgCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCt  >  1:2786941/1‑69 (MQ=255)
                                                            |                                                                  
TAACGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCT  >  minE/72830‑72956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: