| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 740,069 | C→G | 34.0% | S246S (TCG→TCC) | fhuE ← | ferric‑rhodotorulic acid outer membrane transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 740,069 | 0 | C | G | 34.0% | 97.2 / 74.0 | 97 | S246S (TCG→TCC) | fhuE | ferric‑rhodotorulic acid outer membrane transporter |
| Reads supporting (aligned to +/- strand): ref base C (49/15); new base G (30/3); total (79/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
AATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAA > minE/740003‑740136 | aaggcgcTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCg > 1:1461141/4‑68 (MQ=255)aaTGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCg > 1:186307/1‑68 (MQ=255)aaTGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCg > 1:1730249/1‑68 (MQ=255)aaTGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCg > 1:3093899/1‑68 (MQ=255)aaTGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCg > 1:1768701/1‑68 (MQ=255)aaTGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCg > 1:3176334/1‑68 (MQ=255)aaTGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCg > 1:1827986/1‑68 (MQ=255)aaTGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCg > 1:1830411/1‑68 (MQ=255)aaTGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCg > 1:3330765/1‑68 (MQ=255)aaTGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCg > 1:1381926/1‑68 (MQ=255)aaTGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCg > 1:1268540/1‑68 (MQ=255)aaTGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCg > 1:525542/1‑68 (MQ=255)aaTGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCg > 1:2469168/1‑68 (MQ=255)aaTGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCg > 1:2291003/1‑68 (MQ=255)aaTGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCg > 1:2092162/1‑68 (MQ=255)aaTGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCg > 1:897495/1‑68 (MQ=255)aaTGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCg > 1:2154517/1‑68 (MQ=255) cgcTGATATTCGTAACCGGCTGAAAGCGTGGTAAGATCGCCTAAATCAGCATCGACAATGCCCgaga > 1:2006449/1‑67 (MQ=255) cgcTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCgaga > 1:1584982/1‑67 (MQ=255) ttCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCtt < 1:997458/69‑1 (MQ=255) ttCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCtt < 1:2540824/69‑1 (MQ=255) ttCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCtt < 1:1469272/69‑1 (MQ=255) ttCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCtt < 1:668248/69‑1 (MQ=255) tCGTACCCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATAGACAATGCCCGAGAAGAAGGTCtt < 1:243381/68‑1 (MQ=255) tCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCtt < 1:248952/68‑1 (MQ=255) cGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCtttt > 1:2177172/1‑69 (MQ=255) cGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCtttt > 1:2673882/1‑69 (MQ=255) cGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCtttt > 1:1068031/1‑69 (MQ=255) cGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCtttt > 1:3406992/1‑69 (MQ=255) tAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTc > 1:1062725/1‑68 (MQ=255) tAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTc > 1:1358079/1‑68 (MQ=255) tAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCa > 1:2370078/1‑69 (MQ=255) tAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCa > 1:3053779/1‑69 (MQ=255) tAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCa > 1:2393654/1‑69 (MQ=255) tAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCa > 1:663876/1‑69 (MQ=255) tAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCa > 1:2613031/1‑69 (MQ=255) tAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCa > 1:330973/1‑69 (MQ=255) tAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCa > 1:3004350/1‑69 (MQ=255) tAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCa > 1:3160445/1‑69 (MQ=255) tAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCa > 1:3031922/1‑69 (MQ=255) tAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCa > 1:1054494/1‑69 (MQ=255) tAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCa > 1:2067173/1‑69 (MQ=255) tAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCa > 1:1632631/1‑69 (MQ=255) tAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCa > 1:19716/1‑69 (MQ=255) cTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACtgttgt < 1:3216085/69‑1 (MQ=255) cTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACtgttgt < 1:2258492/69‑1 (MQ=255) cTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACtgttgt < 1:3378050/69‑1 (MQ=255) cTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACtgttgt < 1:2514273/69‑1 (MQ=255) cTGAAAGCGTAGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACtgttgt < 1:1395274/69‑1 (MQ=255) aGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCttt > 1:1930645/1‑54 (MQ=255) aGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCgg > 1:440693/1‑69 (MQ=255) aGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCgg > 1:1732813/1‑69 (MQ=255) aGATCGCCTAAATCAGCATCGACAATGCCGGAGaagaag > 1:2005850/1‑39 (MQ=255) aGATCGCCTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGcc > 1:2853565/1‑67 (MQ=255) aGATCGCCTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGcc > 1:83737/1‑67 (MQ=255) aGATCGCCTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGcc > 1:3017761/1‑67 (MQ=255) aGATCGCCTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGcc > 1:1804617/1‑67 (MQ=255) aGATCGCCTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGcc > 1:2452571/1‑67 (MQ=255) aGATCGCCTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGcc > 1:1361172/1‑67 (MQ=255) aGATCGCCTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGcc > 1:2683093/1‑67 (MQ=255) aGATCGCCTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGcc > 1:2645461/1‑67 (MQ=255) aGATCGCCTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGcc > 1:2625917/1‑67 (MQ=255) aGATCGCCTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGcc > 1:1831858/1‑67 (MQ=255) aGATCGCCTAAATCAGCATCGACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGc > 1:2749172/1‑66 (MQ=255) aGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGcc > 1:3246177/1‑67 (MQ=255) aGATCGCCTAAATCAGCATCGACAATGCAGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGcc > 1:2041321/1‑67 (MQ=255) cGCCTAAATCAGCATCGAGAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGt > 1:1204707/1‑69 (MQ=255) cGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAg > 1:1417215/1‑68 (MQ=255) cGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGt > 1:3010033/1‑69 (MQ=255) cGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGt > 1:1904417/1‑69 (MQ=255) cTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGt < 1:2960048/69‑1 (MQ=255) cTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGt < 1:2221292/69‑1 (MQ=255) cATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGt < 1:747049/69‑1 (MQ=255) cATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGt < 1:444441/69‑1 (MQ=255) taCAATGCCGGAGAAGAAGGTCTTTTAACTGTTGTAGCGGTCCAGCCa > 1:3053840/2‑48 (MQ=38) gACTATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt > 1:1636070/1‑49 (MQ=39) gACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt > 1:1163294/1‑49 (MQ=255) gACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt > 1:105176/1‑49 (MQ=255) gACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt > 1:733693/1‑49 (MQ=255) gACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt > 1:355282/1‑49 (MQ=255) gACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt > 1:1526222/1‑49 (MQ=255) gACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt > 1:3047244/1‑49 (MQ=255) gACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt > 1:2779213/1‑49 (MQ=255) gACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt > 1:1813273/1‑49 (MQ=255) gACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt > 1:2093907/1‑49 (MQ=255) gACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt > 1:2300653/1‑49 (MQ=255) gACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt > 1:2473706/1‑49 (MQ=255) gACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt > 1:2343505/1‑49 (MQ=255) gACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt > 1:1520715/1‑49 (MQ=255) gACAATGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt > 1:1046118/1‑49 (MQ=255) gACAATGCCGGACAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCAt > 1:2024373/1‑49 (MQ=255) aaTGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAgccgcc < 1:451213/69‑1 (MQ=255) aaTGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAgccgcc < 1:356382/69‑1 (MQ=255) aaTGCCGGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAgccgcc < 1:2104338/69‑1 (MQ=255) aaTGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAgccgcc > 1:1133968/1‑69 (MQ=255) gCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTAtt > 1:2634454/1‑54 (MQ=255) gCCCGAGAAGAAGGTCTTTTCACTGCTGTAGCGGTCCAGCCATGAGTCGTTAtt > 1:2360853/1‑54 (MQ=255) cGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACaa < 1:688652/68‑1 (MQ=255) | AATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAA > minE/740003‑740136 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |