| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 740,600 | G→T | 57.4% | T69T (ACC→ACA) | fhuE ← | ferric‑rhodotorulic acid outer membrane transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 740,600 | 0 | G | T | 57.4% | 11.6 / 54.1 | 54 | T69T (ACC→ACA) | fhuE | ferric‑rhodotorulic acid outer membrane transporter |
| Reads supporting (aligned to +/- strand): ref base G (17/6); new base T (13/18); total (30/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.75e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01 | |||||||||||
CTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGT > minE/740533‑740656 | cTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTgg > 1:3238381/1‑69 (MQ=255)cTTCCATCCGATGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTgg > 1:3193766/1‑69 (MQ=255) aTCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTgg < 1:2627583/64‑1 (MQ=255) aTCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGt < 1:2858671/65‑1 (MQ=255) aTCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGt < 1:1583360/65‑1 (MQ=255) aTCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGt < 1:1772849/65‑1 (MQ=255) tAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGAc > 1:1204978/1‑68 (MQ=255) tAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGAc > 1:73468/1‑68 (MQ=255) tAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGAc > 1:3258400/1‑68 (MQ=255) tAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGAc > 1:3219051/1‑68 (MQ=255) tAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGAc > 1:2843407/1‑68 (MQ=255) tAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGAc > 1:2628113/1‑68 (MQ=255) tAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGAc > 1:2619813/1‑68 (MQ=255) tAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGAc > 1:2605515/1‑68 (MQ=255) tAGTGCCCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGt > 1:2348783/1‑64 (MQ=255) tAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACgt > 1:225144/1‑68 (MQ=255) tAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACgt > 1:2211136/1‑68 (MQ=255) tAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACgt > 1:860008/1‑68 (MQ=255) tAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACgt > 1:2951435/1‑68 (MQ=255) tAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACgt > 1:2944644/1‑68 (MQ=255) tAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACgt > 1:1658391/1‑68 (MQ=255) tAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACgt > 1:2793733/1‑68 (MQ=255) tAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACgt > 1:2710157/1‑68 (MQ=255) gTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCt > 1:426427/1‑68 (MQ=255) gTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCt > 1:1272452/1‑68 (MQ=255) tCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCAtt > 1:842560/1‑59 (MQ=255) tCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCcatca > 1:2327669/1‑69 (MQ=255) tCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCcatca > 1:1129668/1‑69 (MQ=255) tCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCcatca > 1:2336102/1‑69 (MQ=255) tCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCcatca > 1:3383810/1‑69 (MQ=255) tCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCcatca > 1:96843/1‑69 (MQ=255) tCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCcatca > 1:1296811/1‑69 (MQ=255) cACGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTCGCcatcat > 1:2578302/1‑69 (MQ=255) cACGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTCGCcatcat > 1:1522627/1‑69 (MQ=255) cACGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTCGCcatcat > 1:498142/1‑69 (MQ=255) cACGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTCGCcatcat > 1:1851978/1‑69 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:148533/60‑1 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:803712/60‑1 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:775302/60‑1 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:714515/60‑1 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:559543/60‑1 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:242033/60‑1 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:1393703/60‑1 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:2452634/60‑1 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:2951010/60‑1 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:2457302/60‑1 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:2893267/60‑1 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:2817377/60‑1 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:216792/60‑1 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:1919548/60‑1 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:173592/60‑1 (MQ=255) aCGTTGAGTCATCCGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:3183835/60‑1 (MQ=255) cGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:2398404/59‑1 (MQ=255) gTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGc < 1:991480/68‑1 (MQ=255) cATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCtgt < 1:540980/69‑1 (MQ=255) cATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCtgt < 1:2685292/69‑1 (MQ=255) tGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:296950/47‑1 (MQ=255) | CTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGT > minE/740533‑740656 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |