| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 740,625 | C→T | 43.9% | S61N (AGC→AAC) | fhuE ← | ferric‑rhodotorulic acid outer membrane transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 740,625 | 0 | C | T | 43.9% | 11.9 / 35.4 | 50 | S61N (AGC→AAC) | fhuE | ferric‑rhodotorulic acid outer membrane transporter |
| Reads supporting (aligned to +/- strand): ref base C (25/3); new base T (4/18); total (29/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.82e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
GTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAG > minE/740559‑740688 | gTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCt > 1:1272452/1‑68 (MQ=255)gTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCt > 1:426427/1‑68 (MQ=255) tCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCAtt > 1:842560/1‑59 (MQ=255) tCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCcatca > 1:1129668/1‑69 (MQ=255) tCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCcatca > 1:2327669/1‑69 (MQ=255) tCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCcatca > 1:2336102/1‑69 (MQ=255) tCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCcatca > 1:3383810/1‑69 (MQ=255) tCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCcatca > 1:1296811/1‑69 (MQ=255) tCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCcatca > 1:96843/1‑69 (MQ=255) cACGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTCGCcatcat > 1:1522627/1‑69 (MQ=255) cACGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTCGCcatcat > 1:2578302/1‑69 (MQ=255) cACGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTCGCcatcat > 1:1851978/1‑69 (MQ=255) cACGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTCGCcatcat > 1:498142/1‑69 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:2817377/60‑1 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:2457302/60‑1 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:2452634/60‑1 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:2951010/60‑1 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:242033/60‑1 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:2893267/60‑1 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:216792/60‑1 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:559543/60‑1 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:1919548/60‑1 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:714515/60‑1 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:173592/60‑1 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:775302/60‑1 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:803712/60‑1 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:148533/60‑1 (MQ=255) aCGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:1393703/60‑1 (MQ=255) aCGTTGAGTCATCCGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:3183835/60‑1 (MQ=255) cGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:2398404/59‑1 (MQ=255) gTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGc < 1:991480/68‑1 (MQ=255) cATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCtgt < 1:2685292/69‑1 (MQ=255) cATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCtgt < 1:540980/69‑1 (MQ=255) tGCATTTTTGTACCCGCAGAGGTAGACGTTACGTTGTAATCATTTTc < 1:296950/47‑1 (MQ=255) aGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTttc > 1:113902/1‑69 (MQ=255) aGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTttc > 1:2061834/1‑69 (MQ=255) gACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTg > 1:2361000/1‑46 (MQ=255) gACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGa > 1:498810/1‑47 (MQ=255) gACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGa > 1:1756946/1‑47 (MQ=255) gACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGa > 1:1475011/1‑47 (MQ=255) gACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGa > 1:1253424/1‑47 (MQ=255) ttACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAAcc > 1:2420823/1‑46 (MQ=255) ttACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTcaacaa > 1:2014844/1‑54 (MQ=255) ttACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAg > 1:419868/1‑69 (MQ=255) ttACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAg > 1:583026/1‑69 (MQ=255) ttACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAg > 1:759087/1‑69 (MQ=255) ttACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAg > 1:171742/1‑69 (MQ=255) ttACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAg > 1:1667502/1‑69 (MQ=255) ttACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAg > 1:2869591/1‑69 (MQ=255) ttACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAAACACCGTTTCTTCAg > 1:824540/1‑69 (MQ=255) | GTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAG > minE/740559‑740688 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |