| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 745,073 | C→T | 32.7% | intergenic (+133/‑267) | ycfP → / → ndh | conserved hypothetical protein/respiratory NADH dehydrogenase 2/cupric reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 745,073 | 0 | C | T | 32.7% | 52.7 / 30.9 | 64 | intergenic (+133/‑267) | ycfP/ndh | conserved hypothetical protein/respiratory NADH dehydrogenase 2/cupric reductase |
| Reads supporting (aligned to +/- strand): ref base C (4/39); new base T (14/7); total (18/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.18e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01 | |||||||||||
AGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAAC > minE/745006‑745130 | aGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACg < 1:1460074/69‑1 (MQ=255)aGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACg < 1:2384987/69‑1 (MQ=255) gCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGcc < 1:2908361/69‑1 (MQ=255) gCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGcc < 1:1878002/69‑1 (MQ=255) gCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGcc < 1:1152572/69‑1 (MQ=255) gCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGcc < 1:1881942/69‑1 (MQ=255) gCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGcc < 1:1313805/69‑1 (MQ=255) cACGCCGCATCCGACAGTCGAGCATGAATGCCTGATGTGCTTCTTATCAGGCCTACCGAACGCCCTGCa < 1:3170313/69‑1 (MQ=255) cACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGTGCTTCTTATCAGGCCTACCGAACGCCCTGCa < 1:1267154/69‑1 (MQ=255) cACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGTGCTTCTTATCAGGCCTACCGAACGCCCTGCa < 1:122114/69‑1 (MQ=255) cACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGTGCTTCTTATCAGGCCTACCGAACGCCCTGCa < 1:3010651/69‑1 (MQ=255) cgccgcATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATa < 1:2171373/69‑1 (MQ=255) cgccgcATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATa < 1:440980/69‑1 (MQ=255) cgccgcATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATa < 1:1205216/69‑1 (MQ=255) cgccgcATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATa < 1:767147/69‑1 (MQ=255) cgccgcATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATa < 1:1458066/69‑1 (MQ=255) cgccgcATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATa < 1:632879/69‑1 (MQ=255) gcATCCGACAGTCGAGCATCAATGCCTGATGCGCCTCTTATCAGGCCTACCGAATGCCCTCCATacaca > 1:2400509/1‑68 (MQ=38) gcATCCGACAGTCGAGCATCAATGCCTGATGCGCCTCTTATCAGGCCTACCGAATGCCCTCCATacaca > 1:37517/1‑68 (MQ=38) gcATCCGACAGTCGAGCATCAATGCCTGATGCGCCTCTTATCAGGCCTACCGAATGCCCTCCATacaca > 1:3181768/1‑68 (MQ=38) gcATCCGACAGTCGAGCATCAATGCCTGATGCGCCTCTTATCAGGCCTACCGAATGCCCTCCATacaca > 1:2307458/1‑68 (MQ=38) gcATCCGACAGTCGAGCATCAATGCCTGATGCGCCTCTTATCAGGCCTACCGAATGCCCTCCATacaca > 1:2384500/1‑68 (MQ=38) gcATCCGACAGTCGAGCATCAATGCCTGATGCGCCTCTTATCAGGCCTACCGAATGCCCTCCATacaca > 1:725454/1‑68 (MQ=38) gcATCCGACAGTCGAGCATCAATGCCTGATGCGCCTCTTATCAGGCCTACCGAATGCCCTCCATacaca > 1:1441345/1‑68 (MQ=38) gcATCCGACAGTCGAGCATCAATGCCTGATGCGCCTCTTATCAGGCCTACCGAATGCCCTCCATacaca > 1:2698100/1‑68 (MQ=38) gcATCCGACAGTCGAGCATCAATGCCTGATGCGCCTCTTATCAGGCCTACCGAATGCCCTCCATacaca > 1:1347711/1‑68 (MQ=38) gcATCCGACAGTCGAGCATCAATGCCTGATGCGCCTCTTATCAGGCCTACCGAATGCCCTCCATacaca > 1:1251182/1‑68 (MQ=38) gcATCCGACAGTCGAGCATCAATGCCTGATGCGCCTCTTATCAGGCCTACCGAATGCCCTCCATacaca > 1:1132169/1‑68 (MQ=38) gcATCCGACAGTCGAGCATCAATGCCTGATGCGCCTCTTATCAGGCCTACCGAATGCCCTCCATacaca > 1:2830414/1‑68 (MQ=38) gcATCCGACAGTCGAGCATCAATGCCTGATGCGCCTCTTATCAGGCCTACCGAATGCCCTCCATacac > 1:1327001/1‑68 (MQ=38) gcATCCGACAGTCGAGCATCAATGCCTGATGCGCCTCTTATCAGGCCTACCGAATGCCCTCCATacac > 1:1025343/1‑68 (MQ=38) cAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCActc < 1:549887/69‑1 (MQ=255) cAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCActc < 1:2906240/69‑1 (MQ=255) tCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCActc < 1:1204271/66‑1 (MQ=255) tCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCtat < 1:2869593/69‑1 (MQ=255) cGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCtat < 1:1001204/68‑1 (MQ=255) gCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCa < 1:2971018/48‑1 (MQ=255) cATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCAc > 1:3404765/1‑69 (MQ=255) cATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCAc > 1:535170/1‑69 (MQ=255) ccTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACaa > 1:1814275/1‑69 (MQ=255) ccTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACaa > 1:1375572/1‑69 (MQ=255) tGCTCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCActct < 1:3309866/59‑1 (MQ=255) tGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCActct < 1:795516/59‑1 (MQ=255) tGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCActct < 1:1004564/59‑1 (MQ=255) tGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCActct < 1:2468715/59‑1 (MQ=255) tGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCActct < 1:226736/59‑1 (MQ=255) tGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCActct < 1:18435/59‑1 (MQ=255) tGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCActct < 1:14381/59‑1 (MQ=255) tGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCActct < 1:1767813/59‑1 (MQ=255) ttATCATGACTACCGAACGCCCTGCATACACCCCTCActc < 1:641559/40‑1 (MQ=255) aGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATaataaa < 1:2516337/69‑1 (MQ=255) aGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATaataaa < 1:886163/69‑1 (MQ=255) aGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATaataaa < 1:140745/69‑1 (MQ=255) aGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATaataaa < 1:2055170/69‑1 (MQ=255) aGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATaataaa < 1:2049674/69‑1 (MQ=255) aGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATaataaa < 1:1936844/69‑1 (MQ=255) ggCCTACCGAACGCCGTGCATACACCGCTCTCTCTATATCACTCTCACAAATTCGCTCAAATaataaa < 1:1861257/68‑1 (MQ=255) gCCTACCGAATGCCCTCCATACACACCTCACTCTATATCACTCTCACAAATCAGCTCAAATaataaac < 1:932385/68‑1 (MQ=255) gCCTACCGAATGCCCTCCATACACACCTCACTCTATATCACTCTCACAAATCAGCTCAAATaataaac < 1:2619117/68‑1 (MQ=255) gCCTACCGAATGCCCTCCATACACACCTCACTCTATATCACTCTCACAAATCAGCTCAAATaataaac < 1:1597807/68‑1 (MQ=255) gCCTACCGAATGCCCTCCATACACACCTCACTCTATATCACTCTCACAAATCAGCTCAAATaataaac < 1:598571/68‑1 (MQ=255) gCCTACCGAATGCCCTCCATACACACCTCACTCTATATCACTCTCACAAATCAGCTCAAATaataaac < 1:2187524/68‑1 (MQ=255) gCCTACCGAATGCCCTCCATACACACCTCACTCTATATCACTCTCACAAATCAGCTCAAATaataaac < 1:2340632/68‑1 (MQ=255) gCCTACCGAATGCCCTCCATACACACCTCACTCTATATCACTCTCACAAATCAGCTCAAATaataaac < 1:2567040/68‑1 (MQ=255) | AGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAAC > minE/745006‑745130 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |