| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 746,152 | A→G | 59.6% | V271V (GTA→GTG) | ndh → | respiratory NADH dehydrogenase 2/cupric reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 746,152 | 0 | A | G | 59.6% | 13.2 / 21.9 | 37 | V271V (GTA→GTG) | ndh | respiratory NADH dehydrogenase 2/cupric reductase |
| Reads supporting (aligned to +/- strand): ref base A (6/9); new base G (14/8); total (20/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATGGCGAATATATTGAGGCTGATCTGATGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACCGTATCA > minE/746086‑746219 | cACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATGGCGAATATATTGAGGCTGATCTGATGGTATg > 1:2329544/1‑69 (MQ=255)cACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATGGCGAATATATTGAGGCTGATCTGATGGTATg > 1:614081/1‑69 (MQ=255) aaGGCGGCCTGCACACTAAAGATGGCGAATATATTGAGGCTGATCTGATGGTGTGGGCAGCCGGGATCa < 1:987766/69‑1 (MQ=255) aaGGCGGCCTGCACACTAAAGATGGCGAATATATTGAGGCTGATCTGATGGTGTGGGCAGCCGGGATCa < 1:2577765/69‑1 (MQ=255) aaGGCGGCCTGCACACTAAAGATGGCGAATATATTGAGGCTGATCTGATGGTGTGGGCAGCCGGGATCa < 1:2778160/69‑1 (MQ=255) aaGGCGGCCTGCACACTAAAGATGGCGAATATATTGAGGCTGATCTGATGGTGTGGGCAGCCGGGATCa < 1:1973977/69‑1 (MQ=255) aaGGCGGCCTGCACACTAAAGATGGCGAATATATTGAGGCTGATCTGATGGTGTGGGCAGCCGGGATCa < 1:1935824/69‑1 (MQ=255) aaGGCGGCCTGCACACTAAAGATGGCGAATATATTGAGGCTGATCTGATGGTGTGGGCAGCCGGGATCa < 1:3256437/69‑1 (MQ=255) aaGGCGGCCTGCACACTAAAGATGGCGAATATATTGAGGCTGATCTGATGGTGTGGGCAGCCGGGATCa < 1:352719/69‑1 (MQ=255) aaGGCGGCCTGCACACTAAAGATGGCGAATATATTGAGGCTGATCTGATGGTATGGGCAGCCGGGAt > 1:1267218/1‑67 (MQ=255) aGGCGGCCTGCACACTAAAGATGGCGAATATATTGAGGCTGATCTGATGGTGTGGGCAGCCGGGATCa < 1:1949596/68‑1 (MQ=255) ggcggcCTGCACACTAAAGATGGCGAATATATTGAGGCTGATCTGATGGTATGGGCAGCCGTGATCaa < 1:6401/68‑1 (MQ=255) aaaGATGGCGAATATATTGAGGCTGATCTGATGGTGTGGGCa > 1:448720/1‑42 (MQ=255) aaaGATGGCGAATATATTGAGGCTGATCTGATGGTGTGGGCAGCCGGGATCAAAGCGCCTGCCTTCCt > 1:2795012/1‑68 (MQ=255) aaaGATGGCGAATATATTGAGGCTGATCTGATGGTGTGGGCAGCCGGGATCAAAGCGCCTGACTTcc > 1:2985171/1‑67 (MQ=255) aaaGATGGCGAATATATTGAGGCTGATCTGATGGTGTGGGCAGCCGGGATCAAAGCGCCTGACTTCCt > 1:3002597/1‑68 (MQ=255) aaaGATGGCGAATATATTGAGGCTGATCTGATGGTGTGGGCAGCCGGGATCAAAGCGCCTGACTTCCt > 1:2977802/1‑68 (MQ=255) aaaGATGGCGAATATATTGAGGCTGATCTGATGGTGTGGGCAGCCGGGATCAAAGCGCCTGACTTCCt > 1:2937578/1‑68 (MQ=255) aaaGATGGCGAATATATTGAGGCTGATCTGATGGTGTGGGCAGCCGGGATCAAAGCGCCTGACTTCCt > 1:1110469/1‑68 (MQ=255) aaaGATGGCGAATATATTGAGGCTGATCTGATGGTGTGGGCAGCCGGGATCAAAGCGCCTGACTTCCt > 1:185524/1‑68 (MQ=255) aaaGATGGCGAATATATTGAGGCTGATCTGATGGTGTGGGCAGCCGGGATCAAAGCGCCTGACTTCCt > 1:1091745/1‑68 (MQ=255) aaaGATGGCGAATATATTGAGGCTGATCTGATGGTGTGGGCAGCCGGGATCAAAGCGCCTGACTTCCt > 1:1413857/1‑68 (MQ=255) aaaGATGGCGAATATATTGAGGCTGATCTGATGGTGTGGGCAGCCGGGATCAAAGCGCCTGACTTCCt > 1:1722197/1‑68 (MQ=255) aaaGATGGCGAATATATTGAGGCTGATCTGATGGTGTGGGCAGCCGGGATCAAAGCGCCTGACTTCCt > 1:254716/1‑68 (MQ=255) aaaGATGGCGAATATATTGAGGCTGATCTGATGGTGTGGGCAGCCGGGATCAAAGCGCCTGACTTCCt > 1:2089383/1‑68 (MQ=255) aaaGATGGCGAATATATTGAGGCTGATCTGATGGTGTGGGCAGCCGGGATCAAAGCGCCTGACTTCCt > 1:2148212/1‑68 (MQ=255) aaGATGGCGAATATATTGAGGCTGATCTGATGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCt > 1:1857969/1‑67 (MQ=255) aaGATGGCGAATATATTGAGGCTGATCTGATGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCt > 1:2992182/1‑67 (MQ=255) aaGATGGCGAATATATTGAGGCTGATCTGATGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCt > 1:2622822/1‑67 (MQ=255) gCGAATATATTGAGGCTGATCTGATGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGaaa < 1:1288606/65‑1 (MQ=255) tatatTGAGGCTGATCTGATGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCggt < 1:3178234/69‑1 (MQ=255) tatatTGAGGCTGATCTGATGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCggt < 1:1742472/69‑1 (MQ=255) tatatTGAGGCTGATCTGATGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCggt < 1:3298685/69‑1 (MQ=255) tatatTGAGGCTGATCTGATGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCggt < 1:2251173/69‑1 (MQ=255) tatatTGAGGCTGATCTGATGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCggt < 1:781198/69‑1 (MQ=255) tatatTGAGGATGATCTGATGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCggt < 1:1664316/69‑1 (MQ=255) atatTGAGGCTGATCTGATGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCggt < 1:674574/68‑1 (MQ=255) aTGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACCGTATCa < 1:1964760/68‑1 (MQ=255) aTGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACCGTATCa < 1:2782783/68‑1 (MQ=255) aTGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACCGTATCa < 1:2759206/68‑1 (MQ=255) | CACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATGGCGAATATATTGAGGCTGATCTGATGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACCGTATCA > minE/746086‑746219 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |