Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 749,132 T→C 36.5% K166K (AAA→AAG ycfS ← conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE749,1320TC36.5% 29.2 / 30.4 52K166K (AAA→AAGycfSconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (18/15);  new base C (12/7);  total (30/22)
Fisher's exact test for biased strand distribution p-value = 5.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

ATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTG  >  minE/749069‑749198
                                                               |                                                                  
atgatgGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTtata                                                               <  1:2868635/69‑1 (MQ=255)
atgatgGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTtata                                                               <  1:1249325/69‑1 (MQ=255)
atgatgGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTtata                                                               <  1:3156417/69‑1 (MQ=255)
         cATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGcgcg                                                      <  1:734711/69‑1 (MQ=255)
         cATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGcgcg                                                      <  1:3022119/69‑1 (MQ=255)
         cATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGcgcg                                                      <  1:2003491/69‑1 (MQ=255)
             gggTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACg                                                            <  1:2478226/59‑1 (MQ=255)
             gggTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACg                                                            <  1:2117061/59‑1 (MQ=255)
             gggTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACg                                                            <  1:1290839/59‑1 (MQ=255)
             gggTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACg                                                            <  1:3151993/59‑1 (MQ=255)
             gggTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACg                                                            <  1:1891946/59‑1 (MQ=255)
             gggTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATg                                                  <  1:1870067/69‑1 (MQ=255)
                        tccagCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTgg                                       >  1:1003821/1‑69 (MQ=255)
                          cagCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCttt                                                                  >  1:3301750/1‑40 (MQ=255)
                          cagCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACgg                                                           >  1:2432892/1‑47 (MQ=255)
                          cagCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCgg                                                     >  1:3099199/1‑53 (MQ=255)
                          cagCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCg                                     >  1:159926/1‑69 (MQ=255)
                          cagCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCg                                     >  1:3395005/1‑69 (MQ=255)
                          cagCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCg                                     >  1:2743052/1‑69 (MQ=255)
                          cagCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCg                                     >  1:2560773/1‑69 (MQ=255)
                                  aCTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGcgcg                                                      <  1:1334909/44‑1 (MQ=255)
                                     aCCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTggg                          >  1:1705995/1‑69 (MQ=255)
                                      ccGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGt                         >  1:3185245/1‑69 (MQ=255)
                                        gCAGGCAACTCAATTCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGtt                        >  1:823050/1‑68 (MQ=37)
                                        gCAGGCAACTCAATTCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGtt                        >  1:50647/1‑68 (MQ=37)
                                        gCAGGCAACTCAATTCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGtt                        >  1:546996/1‑68 (MQ=37)
                                        gCAGGCAACTCAATTCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGtt                        >  1:2565187/1‑68 (MQ=37)
                                        gCAGGCAACTCAATTCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGtt                        >  1:103321/1‑68 (MQ=37)
                                        gCAGGCAACTCAATTCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGtt                        >  1:1204525/1‑68 (MQ=37)
                                        gCAGGCAACTCAATTCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGtt                        >  1:2330652/1‑68 (MQ=37)
                                        gCAGGCAACTCAATTCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGtt                        >  1:2197876/1‑68 (MQ=37)
                                        gCAGGCAACTCAATTCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGtt                        >  1:1768602/1‑68 (MQ=37)
                                        gCAGGCAACTCAATTCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGtt                        >  1:2030685/1‑68 (MQ=37)
                                        gCAGGCAACTCAATTCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGt                         >  1:1275993/1‑67 (MQ=37)
                                        gCAGGCAACTCAATTCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGt                         >  1:328981/1‑67 (MQ=37)
                                           ggCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCa                    <  1:2360847/69‑1 (MQ=255)
                                                  cAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTc                                   <  1:2057320/47‑1 (MQ=255)
                                                  cAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTc                                   >  1:439209/1‑47 (MQ=255)
                                                  cAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTc                                   >  1:3337985/1‑47 (MQ=255)
                                                  cAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTc                                   >  1:1999972/1‑47 (MQ=255)
                                                  cAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTc                                   >  1:3106235/1‑47 (MQ=255)
                                                      tCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGa         >  1:2692866/1‑69 (MQ=255)
                                                      tCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGa         >  1:345786/1‑69 (MQ=255)
                                                      tCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGa         >  1:552075/1‑69 (MQ=255)
                                                      tCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGCTTGTCAGa         <  1:2176335/69‑1 (MQ=25)
                                                      tCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGCTTGTCAGa         <  1:2942480/69‑1 (MQ=25)
                                                      tCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGCTTGTCAGa         <  1:1302466/69‑1 (MQ=25)
                                                      tCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGCTTGTCAGa         <  1:2678143/69‑1 (MQ=25)
                                                      tCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGCTTGTCAGa         <  1:1205985/69‑1 (MQ=25)
                                                      tCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGCTTGTCAGa         <  1:2674091/69‑1 (MQ=25)
                                                      tCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTACAGGTTGGGTTTGCACGCTTGTCAGa         <  1:1173620/69‑1 (MQ=25)
                                                              cTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACggtg  >  1:3081829/1‑68 (MQ=255)
                                                              cTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACggtg  >  1:2366282/1‑68 (MQ=255)
                                                               |                                                                  
ATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTG  >  minE/749069‑749198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: