Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 749,147 G→A 48.7% I161I (ATC→ATT ycfS ← conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE749,1470GA48.7% ‑2.4 / 32.0 39I161I (ATC→ATTycfSconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (16/4);  new base A (12/7);  total (28/11)
Fisher's exact test for biased strand distribution p-value = 3.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGT  >  minE/749082‑749213
                                                                 |                                                                  
gggTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATg                                                                 <  1:1870067/69‑1 (MQ=255)
           tccagCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTgg                                                      >  1:1003821/1‑69 (MQ=255)
             cagCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCgg                                                                    >  1:3099199/1‑53 (MQ=255)
             cagCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCg                                                    >  1:3395005/1‑69 (MQ=255)
             cagCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCg                                                    >  1:159926/1‑69 (MQ=255)
             cagCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCg                                                    >  1:2743052/1‑69 (MQ=255)
             cagCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCg                                                    >  1:2560773/1‑69 (MQ=255)
                        aCCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTggg                                         >  1:1705995/1‑69 (MQ=255)
                         ccGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGt                                        >  1:3185245/1‑69 (MQ=255)
                           gCAGGCAACTCAATTCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGtt                                       >  1:103321/1‑68 (MQ=37)
                           gCAGGCAACTCAATTCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGtt                                       >  1:823050/1‑68 (MQ=37)
                           gCAGGCAACTCAATTCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGtt                                       >  1:546996/1‑68 (MQ=37)
                           gCAGGCAACTCAATTCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGtt                                       >  1:50647/1‑68 (MQ=37)
                           gCAGGCAACTCAATTCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGtt                                       >  1:2565187/1‑68 (MQ=37)
                           gCAGGCAACTCAATTCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGtt                                       >  1:2330652/1‑68 (MQ=37)
                           gCAGGCAACTCAATTCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGtt                                       >  1:2197876/1‑68 (MQ=37)
                           gCAGGCAACTCAATTCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGtt                                       >  1:1768602/1‑68 (MQ=37)
                           gCAGGCAACTCAATTCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGtt                                       >  1:1204525/1‑68 (MQ=37)
                           gCAGGCAACTCAATTCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGtt                                       >  1:2030685/1‑68 (MQ=37)
                           gCAGGCAACTCAATTCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGt                                        >  1:328981/1‑67 (MQ=37)
                           gCAGGCAACTCAATTCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGt                                        >  1:1275993/1‑67 (MQ=37)
                              ggCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCa                                   <  1:2360847/69‑1 (MQ=255)
                                     cAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTc                                                  <  1:2057320/47‑1 (MQ=255)
                                     cAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTc                                                  >  1:1999972/1‑47 (MQ=255)
                                     cAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTc                                                  >  1:439209/1‑47 (MQ=255)
                                     cAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTc                                                  >  1:3106235/1‑47 (MQ=255)
                                     cAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTc                                                  >  1:3337985/1‑47 (MQ=255)
                                         tCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGa                        >  1:2692866/1‑69 (MQ=255)
                                         tCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGa                        >  1:345786/1‑69 (MQ=255)
                                         tCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGa                        >  1:552075/1‑69 (MQ=255)
                                         tCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGCTTGTCAGa                        <  1:1205985/69‑1 (MQ=25)
                                         tCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGCTTGTCAGa                        <  1:2176335/69‑1 (MQ=25)
                                         tCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGCTTGTCAGa                        <  1:1302466/69‑1 (MQ=25)
                                         tCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGCTTGTCAGa                        <  1:2942480/69‑1 (MQ=25)
                                         tCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGCTTGTCAGa                        <  1:2678143/69‑1 (MQ=25)
                                         tCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGCTTGTCAGa                        <  1:2674091/69‑1 (MQ=25)
                                         tCCCTGTGCCTTATACCGCGCGCGAATGTTTGCCGTTGGCGTACAGGTTGGGTTTGCACGCTTGTCAGa                        <  1:1173620/69‑1 (MQ=25)
                                                 cTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACggtg                 >  1:3081829/1‑68 (MQ=255)
                                                 cTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACggtg                 >  1:2366282/1‑68 (MQ=255)
                                                               cgGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGgt  <  1:3185877/69‑1 (MQ=255)
                                                                 gATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGgt  >  1:3292180/1‑67 (MQ=255)
                                                                 gATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGgt  >  1:710605/1‑67 (MQ=255)
                                                                 |                                                                  
GGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGT  >  minE/749082‑749213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: