| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 75,694 | A→G | 16.8% | L287L (TTA→TTG) | mraW → | S‑adenosyl‑dependent methyltransferase activity on membrane‑located substrates |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 75,694 | 0 | A | G | 16.8% | 101.5 / 11.0 | 65 | L287L (TTA→TTG) | mraW | S‑adenosyl‑dependent methyltransferase activity on membrane‑located substrates |
| Reads supporting (aligned to +/- strand): ref base A (14/40); new base G (8/3); total (22/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.94e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01 | |||||||||||
GTTACCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCAGAGAG > minE/75628‑75762 | gTTACCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAAt > 1:1147992/1‑69 (MQ=255) gagCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAagaaga < 1:2586418/68‑1 (MQ=255) gagCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAagaaga < 1:1348120/68‑1 (MQ=255) gagCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAagaaga < 1:656917/68‑1 (MQ=255) gagCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAagaaga < 1:3297905/68‑1 (MQ=255) gagCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAagaaga < 1:1668319/68‑1 (MQ=255) agcagcTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAagaaga < 1:2047449/67‑1 (MQ=255) cTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCt < 1:1021973/69‑1 (MQ=255) cTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCt < 1:3376307/69‑1 (MQ=255) tGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCgaag < 1:2311719/44‑1 (MQ=255) tGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCgaag < 1:2191269/44‑1 (MQ=255) tGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCgaag < 1:2808839/44‑1 (MQ=255) tGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCgaag < 1:1596012/44‑1 (MQ=255) tGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCgaag < 1:134295/44‑1 (MQ=255) aGCACTAGGTAAGTTGATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCt > 1:1622714/1‑69 (MQ=255) aGCACTAGGTAAGTTGATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCt > 1:3345369/1‑69 (MQ=255) aGCACTAGGTAAGTTGATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCt > 1:3181273/1‑69 (MQ=255) aGCACTAGGTAAGTTGATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCt > 1:433616/1‑69 (MQ=255) aGCACTAGGTAAGTTGATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCt > 1:1883939/1‑69 (MQ=255) aGCACTAGGTAAGTTGATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCt > 1:2564957/1‑69 (MQ=255) aGCACTAGGTAAGTTGATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCt > 1:2545293/1‑69 (MQ=255) aGCACTAGGTAAGTTGATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCt > 1:2141932/1‑69 (MQ=255) aCTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCg < 1:2803122/69‑1 (MQ=255) aCTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCg < 1:2675053/69‑1 (MQ=255) aCTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCg < 1:677758/69‑1 (MQ=255) aCTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCg < 1:772072/69‑1 (MQ=255) aCTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCg < 1:1120939/69‑1 (MQ=255) aCTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCg < 1:2214039/69‑1 (MQ=255) aCTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCg < 1:1124964/69‑1 (MQ=255) aCTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCg < 1:1983054/69‑1 (MQ=255) aCTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCg < 1:1931477/69‑1 (MQ=255) tAGGTAAGTTGTTGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTTCGTa < 1:3413695/69‑1 (MQ=255) tAGGTAAGTTGATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTa < 1:741753/69‑1 (MQ=255) tAGGTAAGTTGATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTa < 1:3234868/69‑1 (MQ=255) aGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTa > 1:748634/1‑68 (MQ=255) aGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTAt > 1:1782616/1‑69 (MQ=255) aGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTAt > 1:611732/1‑69 (MQ=255) aGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTAt > 1:508863/1‑69 (MQ=255) aGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTAt > 1:1496130/1‑69 (MQ=255) aGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTAt > 1:2914045/1‑69 (MQ=255) aGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTAt > 1:2396745/1‑69 (MQ=255) cAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTg < 1:1881734/68‑1 (MQ=255) cAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTg < 1:730085/68‑1 (MQ=255) cAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTg < 1:2836641/68‑1 (MQ=255) cAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTg < 1:1278982/68‑1 (MQ=255) cAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTg < 1:619105/68‑1 (MQ=255) cAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTg < 1:1907849/68‑1 (MQ=255) ttAATGCCGGGCGAAGAAGAGGTGGGTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCagag < 1:2475714/69‑1 (MQ=255) ttAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCagag < 1:2334676/69‑1 (MQ=255) ttAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCagag < 1:2401896/69‑1 (MQ=255) ttAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCagag < 1:1373503/69‑1 (MQ=255) ttAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCagag < 1:2737640/69‑1 (MQ=255) ttAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCagag < 1:3381782/69‑1 (MQ=255) ttAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCagag < 1:1722534/69‑1 (MQ=255) ttAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCagag < 1:3234666/69‑1 (MQ=255) ttAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCagag < 1:2259576/69‑1 (MQ=255) ttAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCagag < 1:301406/69‑1 (MQ=255) ttAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCagag < 1:2733675/69‑1 (MQ=255) ttAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCagag < 1:1863514/69‑1 (MQ=255) tAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCagag > 1:843113/1‑68 (MQ=255) tAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCagag > 1:2675726/1‑68 (MQ=255) tAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCagag > 1:1442243/1‑68 (MQ=255) tAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCagag > 1:249619/1‑68 (MQ=255) tAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCagag > 1:2807230/1‑68 (MQ=255) tAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCACGTGCCCGTAGTTCAGTTCTGCGTATTGCagag > 1:342775/1‑68 (MQ=255) aaTGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCagagag > 1:871729/1‑69 (MQ=255) aaTGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCagagag > 1:197927/1‑69 (MQ=255) aaTGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCagagag > 1:788339/1‑69 (MQ=255) aaTGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCagagag > 1:1712235/1‑69 (MQ=255) aaTGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCagagag > 1:2780851/1‑69 (MQ=255) | GTTACCGATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAAGAAGAGGTGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCAGAGAG > minE/75628‑75762 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |