| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 749,866 | G→A | 30.7% | R1118R (CGC→CGT) | mfd ← | transcription‑repair coupling factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 749,866 | 0 | G | A | 30.7% | 76.7 / 37.0 | 81 | R1118R (CGC→CGT) | mfd | transcription‑repair coupling factor |
| Reads supporting (aligned to +/- strand): ref base G (4/52); new base A (15/10); total (19/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.31e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
TCACGCATAAACTGGCGTACCCATTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGC > minE/749798‑749928 | tCACGCATAAACTGGCGTACCCATTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAgg > 1:1051051/1‑69 (MQ=255) cACGCATAAACTGGCGTACCCATTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGgc > 1:2407816/1‑69 (MQ=255) cACGCATAAACTGGCGTACCCATTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGgc > 1:2515792/1‑69 (MQ=255) cACGCATAAACTGGCGTACCCATTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGgc > 1:1197205/1‑69 (MQ=255) cACGCATAAACTGGCGTACCCATTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGgc > 1:571424/1‑69 (MQ=255) cACGCATAAACTGGCGTACCCATTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGgc > 1:1473935/1‑69 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTTAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:194710/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:3304977/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:3435220/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:3183896/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:3176887/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:3090157/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:3034743/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:2966624/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:2910566/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:2908805/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:2861061/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:2435807/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:3347698/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:2757822/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:420039/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:420428/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:509396/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:527751/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:541642/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:547761/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:604591/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:781130/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:864999/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:945834/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:965540/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:985911/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:2339520/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:2412659/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:1637919/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:1096422/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:1271416/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:151972/69‑1 (MQ=255) aTTCGATACGCGTTTTCCGCTCACTCAAATCATGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGt < 1:2990381/69‑1 (MQ=255) cGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGTAAt < 1:1506793/69‑1 (MQ=255) cGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGTAAt < 1:364191/69‑1 (MQ=255) cGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGTAAt < 1:360225/69‑1 (MQ=255) cGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGTAAt < 1:355267/69‑1 (MQ=255) cGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGTAAt > 1:2329359/1‑69 (MQ=255) cGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGTAAt < 1:2476876/69‑1 (MQ=255) cGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGTAAt > 1:2155669/1‑69 (MQ=255) tACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGTAAtgc < 1:2595058/68‑1 (MQ=255) tACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGTAAtgc < 1:2006992/68‑1 (MQ=255) tcactcaAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTgc < 1:1335019/69‑1 (MQ=255) tcactcaAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTgc < 1:3418802/69‑1 (MQ=255) tcactcaAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTgc < 1:1089827/69‑1 (MQ=255) tcactcaAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTgc < 1:2643118/69‑1 (MQ=255) cactcaAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTgc < 1:1960012/68‑1 (MQ=255) caAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCaa > 1:1277162/1‑69 (MQ=255) caAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCaa > 1:1178388/1‑69 (MQ=255) aTCCTGAATAAACTTCAGACGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAAc < 1:2812642/68‑1 (MQ=255) aTCCTGAATAAACTTCAGACGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAAc < 1:250938/68‑1 (MQ=255) aTCCTGAATAAACTTCAGACGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAAc < 1:1313893/68‑1 (MQ=255) aTCCTGAATAAACTTCAGACGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAAc < 1:1360579/68‑1 (MQ=255) aTCCTGAATAAACTTCAGACGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAAc < 1:367602/68‑1 (MQ=255) aTCCTGAATAAACTTCAGACGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAAc < 1:2819273/68‑1 (MQ=255) aTCCTGAATAAACTTCAGACGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAAc < 1:1100423/68‑1 (MQ=255) aTCCTGAATAAACTTCAGACGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAAc < 1:2854538/68‑1 (MQ=255) aTCCTGAATAAACTTCAGACGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAAc < 1:1644487/68‑1 (MQ=255) aTCCTGAATAAACTTCAGACGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAAc < 1:2491791/68‑1 (MQ=255) tCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCa < 1:899334/69‑1 (MQ=255) tCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCa < 1:2133520/69‑1 (MQ=255) tCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCa < 1:697099/69‑1 (MQ=255) tCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCa < 1:2636353/69‑1 (MQ=255) tCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCa < 1:1199265/69‑1 (MQ=255) tCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCa < 1:2288607/69‑1 (MQ=255) tCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCa < 1:2818197/69‑1 (MQ=255) ttCAGACGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAgga > 1:2046548/1‑67 (MQ=255) ttCAGACGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGc > 1:723731/1‑68 (MQ=255) ttCAGACGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGc > 1:2083519/1‑68 (MQ=255) ttCAGACGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGc > 1:811353/1‑68 (MQ=255) ttCAGACGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGc > 1:2448287/1‑68 (MQ=255) ttCAGACGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGc > 1:1265731/1‑68 (MQ=255) ttCAGACGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGc > 1:262859/1‑68 (MQ=255) ttCAGACGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGc > 1:1438952/1‑68 (MQ=255) ttCAGACGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGc > 1:406983/1‑68 (MQ=255) ttCAGACGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGc > 1:2778616/1‑68 (MQ=255) ttCAGACGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGc > 1:3379349/1‑68 (MQ=255) ttCAGACGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGc > 1:1533786/1‑68 (MQ=255) ttCAGACGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGc > 1:3293738/1‑68 (MQ=255) ttCAGACGCGTCGGACAATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGc > 1:1732701/1‑68 (MQ=255) ttCAGACGCGTCGGACAATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGc > 1:306643/1‑68 (MQ=255) | TCACGCATAAACTGGCGTACCCATTCGATACGCGTTTTCCGCTCACTCAAATCCTGAATAAATTTCAGGCGCGTCGGACCATCAAGGCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGC > minE/749798‑749928 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |