| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 752,626 | G→A | 100% | D198D (GAC→GAT) | mfd ← | transcription‑repair coupling factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 752,626 | 0 | G | A | 75.1% | 39.2 / 9.1 | 36 | D198D (GAC→GAT) | mfd | transcription‑repair coupling factor |
| Reads supporting (aligned to +/- strand): ref base G (2/7); new base A (0/27); total (2/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.71e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCAC > minE/752567‑752693 | ggAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCa < 1:806823/69‑1 (MQ=255)ggAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCa < 1:2719855/69‑1 (MQ=255) tGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCg < 1:273683/68‑1 (MQ=255) tGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCg < 1:563030/68‑1 (MQ=255) tGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCg < 1:2429814/68‑1 (MQ=255) gATTGATCTCTTCTACTTCCTCCAGCGTGCGCTGGCTATCGACg < 1:3286968/44‑1 (MQ=37) gATTGATCTCTTCTACTTCCTCCAGCGTGCGCTGGCTATCGACg < 1:299757/44‑1 (MQ=37) gATTGATCTCTTCTACTTCCTCCAGCGTGCGCTGGCTATCGACg < 1:3051088/44‑1 (MQ=37) gATTGATCTCTTCTACTTCCTCCAGCGTGCGCTGGCTATCGACg < 1:3124493/44‑1 (MQ=37) gATTGATCTCTTCTACTTCCTCCAGCGTGCGCTGGCTATCGACg < 1:312488/44‑1 (MQ=37) gATTGATCTCTTCTACTTCCTCCAGCGTGCGCTGGCTATCGACg < 1:3201267/44‑1 (MQ=37) gATTGATCTCTTCTACTTCCTCCAGCGTGCGCTGGCTATCGACg < 1:3238831/44‑1 (MQ=37) gATTGATCTCTTCTACTTCCTCCAGCGTGCGCTGGCTATCGACg < 1:2972514/44‑1 (MQ=37) gATTGATCTCTTCTACTTCCTCCAGCGTGCGCTGGCTATCGACg < 1:390939/44‑1 (MQ=37) gATTGATCTCTTCTACTTCCTCCAGCGTGCGCTGGCTATCGACg < 1:700202/44‑1 (MQ=37) gATTGATCTCTTCTACTTCCTCCAGCGTGCGCTGGCTATCGACg < 1:7070/44‑1 (MQ=37) gATTGATCTCTTCTACTTCCTCCAGCGTGCGCTGGCTATCGACg < 1:864364/44‑1 (MQ=37) gATTGATCTCTTCTACTTCCTCCAGCGTGCGCTGGCTATCGACg < 1:878918/44‑1 (MQ=37) gATTGATCTCTTCTACTTCCTCCAGCGTGCGCTGGCTATCGACg < 1:977538/44‑1 (MQ=37) gATTGATCTCTTCTACTTCCTCCAGCGTGCGCTGGCTATCGACg < 1:1927790/44‑1 (MQ=37) gATTGATCTCTTCTACTTCCTCCAGCGTGCGCTGGCTATCGACg < 1:1101155/44‑1 (MQ=37) gATTGATCTCTTCTACTTCCTCCAGCGTGCGCTGGCTATCGACg < 1:1558523/44‑1 (MQ=37) gATTGATCTCTTCTACTTCCTCCAGCGTGCGCTGGCTATCGACg < 1:1564572/44‑1 (MQ=37) gATTGATCTCTTCTACTTCCTCCAGCGTGCGCTGGCTATCGACg < 1:1576455/44‑1 (MQ=37) gATTGATCTCTTCTACTTCCTCCAGCGTGCGCTGGCTATCGACg < 1:1823591/44‑1 (MQ=37) gATTGATCTCTTCTACTTCCTCCAGCGTGCGCTGGCTATCGACg < 1:1070682/44‑1 (MQ=37) gATTGATCTCTTCTACTTCCTCCAGCGTGCGCTGGCTATCGACg < 1:1991872/44‑1 (MQ=37) gATTGATCTCTTCTACTTCCTCCAGCGTGCGCTGGCTATCGACg < 1:2009012/44‑1 (MQ=37) gATTGATCTCTTCTACTTCCTCCAGCGTGCGCTGGCTATCGACg < 1:2151956/44‑1 (MQ=37) gATTGATCTCTTCTACTTCCTCCAGCGTGCGCTGGCTATCGACg < 1:2528174/44‑1 (MQ=37) gATTGATCTCTTCTACTTCCTCCAGCGTGCGCTGGCTATCGACg < 1:2938349/44‑1 (MQ=37) gATTGATCTCTTCTACTTCCTCCAGCGTGCGCTGGCTATCGACg < 1:2939489/44‑1 (MQ=37) tGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTtcat > 1:804686/1‑69 (MQ=255) tGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTtcat > 1:1494052/1‑69 (MQ=255) aCTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGa < 1:165941/67‑1 (MQ=255) gCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGAt < 1:3222298/69‑1 (MQ=255) tgTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCAc < 1:2217949/69‑1 (MQ=255) tgTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCAc < 1:831954/69‑1 (MQ=255) | GGAAATTCGTGCGCGGGCAGCAGATTGATCGCTTCTACTTCCTCCAGCGTGCGCTGGCTGTCGACGTCAAACACCCGCAGGCTGTCGATTTCATCATCAAAGAAATCAAGACGATAAGGCAGCTCAC > minE/752567‑752693 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |