| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 753,377 | T→A | 100% | S348S (TCA→TCT) | ycfT ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 753,377 | 0 | T | A | 92.6% | 56.4 / ‑2.0 | 27 | S348S (TCA→TCT) | ycfT | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (0/2); new base A (25/0); total (25/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.85e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAAC > minE/753326‑753445 | cGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGTAGCGGGCAGAGAAGGCGGGGAGaac > 1:780783/1‑67 (MQ=255)cGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGTAGCGGGCAGAGAAGGCGGGGAGaac > 1:3285640/1‑67 (MQ=255)cGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGTAGCGGGCAGAGAAGGCGGGGAGaac > 1:2253004/1‑67 (MQ=255)cGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGTAGCGGGCAGAGAAGGCGGGGAAaac > 1:1702043/1‑67 (MQ=255) ggCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATc < 1:2935673/69‑1 (MQ=255) gCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGa < 1:2452871/67‑1 (MQ=255) gCAGAGAAGGCGGGGAGAACAACAGATCGCTGAATGCGCGCTGTGaa > 1:265309/1‑47 (MQ=39) gCAGAGAAGGCGGGGAGAACAACAGATCGCTGAATGCGCGCTGTGaa > 1:253913/1‑47 (MQ=39) gCAGAGAAGGCGGGGAGAACAACAGATCGCTGAATGCGCGCTGTGaa > 1:272477/1‑47 (MQ=39) gCAGAGAAGGCGGGGAGAACAACAGATCGCTGAATGCGCGCTGTGaa > 1:2780070/1‑47 (MQ=39) gCAGAGAAGGCGGGGAGAACAACAGATCGCTGAATGCGCGCTGTGaa > 1:2817096/1‑47 (MQ=39) gCAGAGAAGGCGGGGAGAACAACAGATCGCTGAATGCGCGCTGTGaa > 1:2832395/1‑47 (MQ=39) gCAGAGAAGGCGGGGAGAACAACAGATCGCTGAATGCGCGCTGTGaa > 1:3080973/1‑47 (MQ=39) gCAGAGAAGGCGGGGAGAACAACAGATCGCTGAATGCGCGCTGTGaa > 1:378444/1‑47 (MQ=39) gCAGAGAAGGCGGGGAGAACAACAGATCGCTGAATGCGCGCTGTGaa > 1:431862/1‑47 (MQ=39) gCAGAGAAGGCGGGGAGAACAACAGATCGCTGAATGCGCGCTGTGaa > 1:529885/1‑47 (MQ=39) gCAGAGAAGGCGGGGAGAACAACAGATCGCTGAATGCGCGCTGTGaa > 1:706690/1‑47 (MQ=39) gCAGAGAAGGCGGGGAGAACAACAGATCGCTGAATGCGCGCTGTGaa > 1:2610857/1‑47 (MQ=39) gCAGAGAAGGCGGGGAGAACAACAGATCGCTGAATGCGCGCTGTGaa > 1:2546155/1‑47 (MQ=39) gCAGAGAAGGCGGGGAGAACAACAGATCGCTGAATGCGCGCTGTGaa > 1:1104092/1‑47 (MQ=39) gCAGAGAAGGCGGGGAGAACAACAGATCGCTGAATGCGCGCTGTGaa > 1:2310699/1‑47 (MQ=39) gCAGAGAAGGCGGGGAGAACAACAGATCGCTGAATGCGCGCTGTGaa > 1:2217346/1‑47 (MQ=39) gCAGAGAAGGCGGGGAGAACAACAGATCGCTGAATGCGCGCTGTGaa > 1:2086372/1‑47 (MQ=39) gCAGAGAAGGCGGGGAGAACAACAGATCGCTGAATGCGCGCTGTGaa > 1:1959397/1‑47 (MQ=39) gCAGAGAAGGCGGGGAGAACAACAGATCGCTGAATGCGCGCTGTGaa > 1:1816435/1‑47 (MQ=39) gCAGAGAAGGCGGGGAGAACAACAGATCGCTGAATGCGCGCTGTGaa > 1:1502647/1‑47 (MQ=39) gCAGAGAAGGCGGGGAGAACAACAGATCGCTGAATGCGCGCTGTGaa > 1:1433549/1‑47 (MQ=39) tGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAAc > 1:2515217/1‑69 (MQ=255) tGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAAc > 1:1801884/1‑69 (MQ=255) tGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAAc > 1:1586980/1‑69 (MQ=255) tGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAAc > 1:1191232/1‑69 (MQ=255) | CGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAAC > minE/753326‑753445 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |