| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 755,221 | C→T | 29.5% | S180S (AGC→AGT) | lolC → | outer membrane‑specific lipoprotein transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 755,221 | 0 | C | T | 29.5% | 82.6 / 34.1 | 81 | S180S (AGC→AGT) | lolC | outer membrane‑specific lipoprotein transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (40/17); new base T (9/15); total (49/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.18e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGC > minE/755154‑755285 | gcgGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGcc > 1:2208615/1‑69 (MQ=255) cAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCt > 1:269629/1‑68 (MQ=255) cAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTg > 1:751234/1‑69 (MQ=255) cAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTg > 1:160750/1‑69 (MQ=255) gTGATGGTGCCATCTGCTAGCCAGTTCACGCCGATGGGGCGTATTCCAAGTCAGCGCCTGTTCAAtgtg < 1:983044/69‑1 (MQ=255) gTGATGGTGCCATCTGCTAGCCAGTTCACGCCGATGGGGCGTATTCCAAGTCAGCGCCTGTTCAAtgtg < 1:2601517/69‑1 (MQ=255) gTGATGGTGCCATCTGCTAGCCAGTTCACGCCGATGGGGCGTATTCCAAGTCAGCGCCTGTTCAAtgtg < 1:2715728/69‑1 (MQ=255) gTGATGGTGCCATCTGCTAGCCAGTTCACGCCGATGGGGCGTATTCCAAGTCAGCGCCTGTTCAAtgtg < 1:2802589/69‑1 (MQ=255) gTGATGGTGCCATCTGCTAGCCAGTTCACGCCGATGGGGCGTATTCCAAGTCAGCGCCTGTTCAAtgtg < 1:1804723/69‑1 (MQ=255) gTGATGGTGCCATCTGCTAGCCAGTTCACGCCGATGGGGCGTATTCCAAGTCAGCGCCTGTTCAAtgtg < 1:3162485/69‑1 (MQ=255) gTGATGGTGCCATCTGCTAGCCAGTTCACGCCGATGGGGCGTATTCCAAGTCAGCGCCTGTTCAAtgtg < 1:3308957/69‑1 (MQ=255) gTGATGGTGCCATCTGCTAGCCAGTTCACGCCGATGGGGCGTATTCCAAGTCAGCGCCTGTTCAAtgtg < 1:3369185/69‑1 (MQ=255) gTGATGGTGCCATCTGCTAGCCAGTTCACGCCGATGGGGCGTATTCCAAGTCAGCGCCTGTTCAAtgtg < 1:908232/69‑1 (MQ=255) gTGATGGTGCCATCTGCTAGCCAGTTCACGCCGATGGGGCGTATTCCAAGTCAGCGCCTGTTCAAtgtg < 1:735242/69‑1 (MQ=255) gTGATGGTGCCATCTGCTAGCCAGTTCACGCCGATGGGGCGTATTCCAAGTCAGCGCCTGTTCAAtgtg < 1:1466372/69‑1 (MQ=255) gTGATGGTGCCATCTGCTAGCCAGTTCACGCCGATGGGGCGTATTCCAAGTCAGCGCCTGTTCAAtgtg < 1:796424/69‑1 (MQ=255) gTGATGGTGCCATCTGCTAGCCAGTTCACGCCGATGGGGCGTATTCCAAGTCAGCGCCTGTTCAAtgtg < 1:1252385/69‑1 (MQ=255) gTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAAtgtg < 1:1558394/69‑1 (MQ=255) gTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAAtgtg < 1:3262208/69‑1 (MQ=255) gTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAAtgtg < 1:2223686/69‑1 (MQ=255) gTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAAtgtg < 1:2117403/69‑1 (MQ=255) tGATGGTGCCATCTGCTAGCCAGTTCACGCCGATGGGGCGTATTCCAAGTCAGCGCCTGTTCAAtgtg < 1:1404449/68‑1 (MQ=255) tGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAAtgtg < 1:2121663/68‑1 (MQ=255) aCCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTc > 1:3356066/1‑55 (MQ=255) aCCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTc > 1:2016786/1‑55 (MQ=255) aCCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTc > 1:2701036/1‑55 (MQ=255) aCCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTc > 1:1941960/1‑55 (MQ=255) aCCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTc > 1:1773697/1‑55 (MQ=255) aCCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTc > 1:1763690/1‑55 (MQ=255) ccATCTGCTAGCCAGTTCACGCCGATGGGGCGTATTCCAAGTCAGCGCCTGTTCAAtgtg < 1:2706755/60‑1 (MQ=255) tgacagccagTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTcgc > 1:3144132/4‑69 (MQ=255) tGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTcgc > 1:1141159/1‑69 (MQ=255) tGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTcgc > 1:2523232/1‑69 (MQ=255) tGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTcgc > 1:1323174/1‑69 (MQ=255) tGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTcgc > 1:467026/1‑69 (MQ=255) tGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTcgc > 1:205725/1‑69 (MQ=255) tGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTcgc > 1:3289394/1‑69 (MQ=255) cACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGa > 1:882883/1‑69 (MQ=255) ccGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTc < 1:219171/69‑1 (MQ=255) ccGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTc < 1:853121/69‑1 (MQ=255) ccGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTc < 1:620347/69‑1 (MQ=255) ccGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTc < 1:378406/69‑1 (MQ=255) ccGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTc < 1:332892/69‑1 (MQ=255) ccGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTc < 1:1940987/69‑1 (MQ=255) cGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTc < 1:3432281/68‑1 (MQ=255) tGGGGCGTATTCCAAGTCAGCGCCTGTTCAATGTGATTGTCACTTTcg > 1:1681791/1‑48 (MQ=39) tGGGGCGTATTCCAAGTCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGAt > 1:867301/1‑68 (MQ=255) tGGGGCGTATTCCAAGTCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATg > 1:2150335/1‑69 (MQ=255) tGGGGCGTATTCCAAGTCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATg > 1:82575/1‑69 (MQ=255) tGGGGCGTATTCCAAGTCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATg > 1:1999123/1‑69 (MQ=255) tGGGGCGTATTCCAAGTCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATg > 1:891081/1‑69 (MQ=255) tGGGGCGTATTCCAAGTCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATg > 1:551830/1‑69 (MQ=255) tGGGGCGTATTCCAAGTCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATg > 1:2710272/1‑69 (MQ=255) tGGGGCGTATTCCAAGTCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATg > 1:3062304/1‑69 (MQ=255) gggCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGc < 1:1736756/69‑1 (MQ=255) gggCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGc < 1:925900/69‑1 (MQ=255) gggCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGc < 1:1919688/69‑1 (MQ=255) gCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTc < 1:2655711/61‑1 (MQ=255) gCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCt < 1:2563520/68‑1 (MQ=255) ttCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGaaa > 1:879995/1‑69 (MQ=255) ttCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGaa > 1:268023/1‑68 (MQ=255) ccAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGAt > 1:2200865/1‑57 (MQ=255) ccAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAAt > 1:171061/1‑68 (MQ=255) ccAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATg > 1:1496152/1‑69 (MQ=255) ccAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATg > 1:1232284/1‑69 (MQ=255) ccAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATg > 1:404335/1‑69 (MQ=255) ccAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATg > 1:400767/1‑69 (MQ=255) ccAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATg > 1:382361/1‑69 (MQ=255) ccAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATg > 1:1501233/1‑69 (MQ=255) ccAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATg > 1:2843186/1‑69 (MQ=255) cAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGt > 1:2171986/1‑52 (MQ=255) cAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATg > 1:1087486/1‑68 (MQ=255) cAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGc > 1:684472/1‑69 (MQ=255) cAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGc > 1:722506/1‑69 (MQ=255) cAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGc > 1:1460837/1‑69 (MQ=255) cAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGc > 1:2584749/1‑69 (MQ=255) cAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGc > 1:3286176/1‑69 (MQ=255) cAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGc > 1:3252037/1‑69 (MQ=255) cAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGc > 1:1782162/1‑69 (MQ=255) cAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGc > 1:950298/1‑69 (MQ=255) cAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGc > 1:976064/1‑69 (MQ=255) cAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGc > 1:2795294/1‑69 (MQ=255) | GCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGC > minE/755154‑755285 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |