| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 756,089 | A→G | 57.7% | P72P (CCA→CCG) | lolD → | outer membrane‑specific lipoprotein transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 756,089 | 0 | A | G | 57.7% | 17.6 / 42.6 | 59 | P72P (CCA→CCG) | lolD | outer membrane‑specific lipoprotein transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (11/14); new base G (19/15); total (30/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
TGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATC > minE/756028‑756153 | tgcACCTGTTAGGCTGGCTGGATACGCCAACCTCCGGCGATGTGATCTTTAACGGTCAACCGATGAGCa > 1:2553855/1‑69 (MQ=255)tgcACCTGTTAGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATCTTTAACGGTCAACCGATGAGCa > 1:1642026/1‑69 (MQ=255)tgcACCTGTTAGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATCTTTAACGGTCAACCGATGAGCa > 1:2502614/1‑69 (MQ=255)tgcACCTGTTAGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATCTTTAACGGTCAACCGATGAGCa > 1:2480684/1‑69 (MQ=255)tgcACCTGTTAGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATCTTTAACGGTCAACCGATGAGCa > 1:2403138/1‑69 (MQ=255)tgcACCTGTTAGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATCTTTAACGGTCAACCGATGAGCa > 1:3322109/1‑69 (MQ=255)tgcACCTGTTAGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATCTTTAACGGTCAACCGATGAGCa > 1:46349/1‑69 (MQ=255)tgcACCTGTTAGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATCTTTAACGGTCAACCGATGAGCa > 1:2733827/1‑69 (MQ=255)tgcACCTGTTAGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATCTTTAACGGTCAACCGATGAGCa > 1:2128401/1‑69 (MQ=255)tgcACCTGTTAGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATCTTTAACGGTCAACCGATGAGCa > 1:329439/1‑69 (MQ=255)tgcACCTGTTAGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATCTTTAACGGTCAACCGATGAGCa > 1:2021065/1‑69 (MQ=255)tgcACCTGTTAGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATCTTTAACGGTCAACCGATGAGCa > 1:1367228/1‑69 (MQ=255)tgcACCTGTTAGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATCTTTAACGGTCAACCGATGAGCa > 1:3289808/1‑69 (MQ=255)tgcACCTGTTAGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATCTTTAACGGTCAACCGATGAGCa > 1:1157410/1‑69 (MQ=255)tgcACCTGTTAGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATCTTTAACGGTCAACCGATGAGCa > 1:3021656/1‑69 (MQ=255)tgcACCTGTTAGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATCTTTAACGGTCAACCGATGAGCa > 1:3009144/1‑69 (MQ=255)tgcACCTGTTAGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATCTTTAACGGTCAACCGATGAGCa > 1:1559039/1‑69 (MQ=255)tgcACCTGTTAGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATCTTTAACGGTCAACCGATGAGCa > 1:1479791/1‑69 (MQ=255)tgcACCTGTTAGGCGGGATGGATACGCCAACCTCCGGCGATGTGATCTTTAAAGGTCAACCGATGAGCa > 1:1810906/1‑69 (MQ=255) gcACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCaa < 1:1526423/69‑1 (MQ=255) gcACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCaa < 1:700827/69‑1 (MQ=255) gcACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCaa < 1:1771860/69‑1 (MQ=255) gcACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCaa < 1:924552/69‑1 (MQ=255) gcACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCaa < 1:2672396/69‑1 (MQ=255) gcACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCaa < 1:1614892/69‑1 (MQ=255) gcACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCaa < 1:1591694/69‑1 (MQ=255) gcACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCaa < 1:1089512/69‑1 (MQ=255) ccTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACt > 1:382598/1‑69 (MQ=255) ccTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACt > 1:639051/1‑69 (MQ=255) gctgGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTgtc > 1:1074869/1‑69 (MQ=255) gctgGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTgtc > 1:2604047/1‑69 (MQ=255) gctgGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTgtc > 1:2270415/1‑69 (MQ=255) gctgGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTgtc > 1:2244984/1‑69 (MQ=255) gctgGGCGCGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTgtc > 1:95558/1‑69 (MQ=255) aCGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGc > 1:342403/1‑68 (MQ=255) cGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCaa > 1:2377204/1‑46 (MQ=255) cGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCaa > 1:472316/1‑46 (MQ=255) cGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCaa > 1:857116/1‑46 (MQ=255) ccAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGa < 1:2549299/68‑1 (MQ=255) cggTCAACCGATGAGCAAACTGTCGTCGGCGGCCAAAGCGGAACTGCGCAACCAGAAGCTGGGAttta < 1:308108/67‑1 (MQ=255) cggTCAACCGATGAGCAAACTGTCGTCGGCGGCCAAAGCGGAACTGCGCAACCAGAAGCTGGGAttta < 1:997947/67‑1 (MQ=255) cggTCAACCGATGAGCAAACTGTCGTCGGCGGCCAAAGCGGAACTGCGCAACCAGAAGCTGGGAttta < 1:1303492/67‑1 (MQ=255) cggTCAACCGATGAGCAAACTGTCGTCGGCGGCCAAAGCGGAACTGCGCAACCAGAAGCTGGGAttta < 1:894667/67‑1 (MQ=255) cggTCAACCGATGAGCAAACTGTCGTCGGCGGCCAAAGCGGAACTGCGCAACCAGAAGCTGGGAttta < 1:1359508/67‑1 (MQ=255) cggTCAACCGATGAGCAAACTGTCGTCGGCGGCCAAAGCGGAACTGCGCAACCAGAAGCTGGGAttta < 1:803276/67‑1 (MQ=255) cggTCAACCGATGAGCAAACTGTCGTCGGCGGCCAAAGCGGAACTGCGCAACCAGAAGCTGGGAttta < 1:2065434/67‑1 (MQ=255) cggTCAACCGATGAGCAAACTGTCGTCGGCGGCCAAAGCGGAACTGCGCAACCAGAAGCTGGGAttta < 1:2262893/67‑1 (MQ=255) cggTCAACCGATGAGCAAACTGTCGTCGGCGGCCAAAGCGGAACTGCGCAACCAGAAGCTGGGAttta < 1:2727273/67‑1 (MQ=255) cggTCAACCGATGAGCAAACTGTCGTCGGCGGCCAAAGCGGAACTGCGCAACCAGAAGCTGGGAttta < 1:3298396/67‑1 (MQ=255) cggTCAACCGATGAGCAAACTGTCGTCGGCGGCCAAAGCGGAACTGCGCAACCAGAAGCTGGGAttta < 1:2743736/67‑1 (MQ=255) cggTCAACCGATGAGCAAACTGTCGTCGGCGGCCAAAGCGGAACTGCGCAACCAGAAGCTGGGAttta < 1:3136977/67‑1 (MQ=255) ggTCAACCGATGAGCAAACTGTCGTCGGCGGCCAAAGCGGAACTGCGCAACCAGAAGCTGGGAttta < 1:2263352/67‑1 (MQ=255) ggTCAACCGATGAGCAAACTGTCGTCGGCGGCCAAAGCGGAACTGCGCAACCAGAAGCTGGGAttta < 1:3308368/67‑1 (MQ=255) tCAACCGATGAGCAAACTGTCGTCGGCGGCCAAAGCGGAACTGCGCAACCAGAAGCTGGGAttta < 1:118272/65‑1 (MQ=255) aGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATc < 1:1026450/69‑1 (MQ=255) aGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATc < 1:49581/69‑1 (MQ=255) aGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATc < 1:1587271/69‑1 (MQ=255) aGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATc < 1:1524356/69‑1 (MQ=255) aGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATc < 1:929011/69‑1 (MQ=255) | TGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATC > minE/756028‑756153 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |