| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 756,787 | G→A | 57.6% | V71V (GTG→GTA) | lolE → | outer membrane‑specific lipoprotein transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 756,787 | 0 | G | A | 57.6% | 17.9 / 48.8 | 66 | V71V (GTG→GTA) | lolE | outer membrane‑specific lipoprotein transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (17/11); new base A (30/8); total (47/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
TTTGAACGCGAACTGAACAACCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGG > minE/756725‑756843 | tttGAACGCTAACTGAACAACCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAg > 1:1273454/1‑69 (MQ=255)tttGAACGCGAACTGAACAACCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAg > 1:561060/1‑69 (MQ=255)tttGAACGCGAACTGAACAACCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAg > 1:2419856/1‑69 (MQ=255)tttGAACGCGAACTGAACAACCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAg > 1:1277254/1‑69 (MQ=255)tttGAACGCGAACTGAACAACCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAg > 1:3339514/1‑69 (MQ=255)tttGAACGCGAACTGAACAACCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAg > 1:2570872/1‑69 (MQ=255)tttGAACGCGAACTGAACAACCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAg > 1:2574019/1‑69 (MQ=255)tttGAACGCGAACTGAACAACCGTATTCTGGCGGTGGCGCCGCATGGTGAAATCGAGGCGGTGGATCAg > 1:1771270/1‑69 (MQ=255) ataacCGCATTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGATCAACTGGc > 1:1962097/3‑69 (MQ=255) ataacCGCATTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGc > 1:3205318/3‑69 (MQ=255) ataacCGCATTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGc > 1:1851772/3‑69 (MQ=255) ataacCGCATTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGc > 1:2480785/3‑69 (MQ=255) ataacCGCATTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGc > 1:2485396/3‑69 (MQ=255) ataacCGCATTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGc > 1:2496292/3‑69 (MQ=255) ataacCGCATTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGc > 1:1726757/3‑69 (MQ=255) ataacCGCATTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGc > 1:1604241/3‑69 (MQ=255) ataacCGCATTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGc > 1:3097310/3‑69 (MQ=255) ataacCGCATTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGc > 1:3348333/3‑69 (MQ=255) ataacCGCATTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGc > 1:3369553/3‑69 (MQ=255) ataacCGCATTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGc > 1:1370479/3‑69 (MQ=255) ataacCGCATTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGc > 1:3395298/3‑69 (MQ=255) ataacCGCATTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGc > 1:431358/3‑69 (MQ=255) ataacCGCATTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGc > 1:643823/3‑69 (MQ=255) caacCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGc > 1:2692861/1‑68 (MQ=255) caacCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGc > 1:92325/1‑68 (MQ=255) caacCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGc > 1:463153/1‑68 (MQ=255) caacCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGc > 1:535677/1‑68 (MQ=255) caacCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGc > 1:1641517/1‑68 (MQ=255) caTTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGCAGGAAg > 1:574101/2‑68 (MQ=255) caTTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGCAGGAAGc > 1:2169032/2‑69 (MQ=255) caTTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGCAGGAAGc > 1:799365/2‑69 (MQ=255) caTTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGCAGGAAGc > 1:573864/2‑69 (MQ=255) caTTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGCAGGAAGc > 1:48117/2‑69 (MQ=255) caTTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGCAGGAAGc > 1:118646/2‑69 (MQ=255) caTTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGCAGGAAGc > 1:1194334/2‑69 (MQ=255) caTTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGCAGGAAGc > 1:1268327/2‑69 (MQ=255) caTTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGCAGGAAGc > 1:1412418/2‑69 (MQ=255) caTTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGCAGGAAGc > 1:1446554/2‑69 (MQ=255) caTTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGCAGGAAGc > 1:1549970/2‑69 (MQ=255) caTTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGCAGGAAGc > 1:2000885/2‑69 (MQ=255) caTTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGCAGGAAGc > 1:1859803/2‑69 (MQ=255) caTTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGCAGGAAGc > 1:2176237/2‑69 (MQ=255) caTTCTGGCGGTAGTACCGCACGGTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGCAGGAAGc > 1:1766529/2‑69 (MQ=255) cTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACt > 1:1086869/1‑68 (MQ=255) cTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTg > 1:1828168/1‑69 (MQ=255) cTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTg < 1:386464/69‑1 (MQ=255) cTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTg > 1:352955/1‑69 (MQ=255) cTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTg < 1:1758355/69‑1 (MQ=255) cTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTg < 1:2415337/69‑1 (MQ=255) cTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTg < 1:254092/69‑1 (MQ=255) cTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTg < 1:1599146/69‑1 (MQ=255) cTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTg < 1:1148013/69‑1 (MQ=255) cTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTg < 1:3068324/69‑1 (MQ=255) cTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTg < 1:2940535/69‑1 (MQ=255) cTGGCGGTAGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTg > 1:351712/1‑69 (MQ=255) tGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTg < 1:1576130/68‑1 (MQ=255) gTGAAATTGAGGCGGTAGATCAGCCGTTGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGc < 1:2656106/69‑1 (MQ=255) gTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGTAAGTGc < 1:439973/69‑1 (MQ=255) gTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGc < 1:353259/69‑1 (MQ=255) gTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGc < 1:1442706/69‑1 (MQ=255) gTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGc < 1:3147865/69‑1 (MQ=255) gTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGc < 1:1134456/69‑1 (MQ=255) gTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGc < 1:2208094/69‑1 (MQ=255) gTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGc < 1:1571300/69‑1 (MQ=255) aTCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCggg < 1:3047302/68‑1 (MQ=255) aTCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCggg < 1:1013386/68‑1 (MQ=255) | TTTGAACGCGAACTGAACAACCGTATTCTGGCGGTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGG > minE/756725‑756843 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |