| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 756,824 | C→A | 100% | H84N (CAC→AAC) | lolE → | outer membrane‑specific lipoprotein transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 756,824 | 0 | C | A | 75.0% | 51.2 / 17.7 | 40 | H84N (CAC→AAC) | lolE | outer membrane‑specific lipoprotein transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (3/7); new base A (0/30); total (3/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.21e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTG > minE/756771‑756893 | gTGAAATTGAGGCGGTAGATCAGCCGTTGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGc < 1:2656106/69‑1 (MQ=255)gTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGTAAGTGc < 1:439973/69‑1 (MQ=255)gTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGc < 1:1134456/69‑1 (MQ=255)gTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGc < 1:353259/69‑1 (MQ=255)gTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGc < 1:3147865/69‑1 (MQ=255)gTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGc < 1:1442706/69‑1 (MQ=255)gTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGc < 1:2208094/69‑1 (MQ=255)gTGAAATTGAGGCGGTAGATCAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGc < 1:1571300/69‑1 (MQ=255) aTCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCggg < 1:1013386/68‑1 (MQ=255) aTCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCggg < 1:3047302/68‑1 (MQ=255) cAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGCCGGGCAtt < 1:1902775/57‑1 (MQ=255) cAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGCCGGGCAtt < 1:1734598/57‑1 (MQ=255) cAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGCCGGGCAtt < 1:2064549/57‑1 (MQ=255) cAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGCCGGGCAtt < 1:2659632/57‑1 (MQ=255) cAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGCCGGGCAtt < 1:2194064/57‑1 (MQ=255) cAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGCCGGGCAtt < 1:2327068/57‑1 (MQ=255) cAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGCCGGGCAtt < 1:2655149/57‑1 (MQ=255) cAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGCCGGGCAtt < 1:2419307/57‑1 (MQ=255) cAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGCCGGGCAtt < 1:2600867/57‑1 (MQ=255) cAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGCCGGGCAtt < 1:1862054/57‑1 (MQ=255) cAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGCCGGGCAtt < 1:2957536/57‑1 (MQ=255) cAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGCCGGGCAtt < 1:1775642/57‑1 (MQ=255) cAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGCCGGGCAtt < 1:1726109/57‑1 (MQ=255) cAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGCCGGGCAtt < 1:1534650/57‑1 (MQ=255) cAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGCCGGGCAtt < 1:1423956/57‑1 (MQ=255) cAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGCCGGGCAtt < 1:1354342/57‑1 (MQ=255) cAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGCCGGGCAtt < 1:1285697/57‑1 (MQ=255) cAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGCCGGGCAtt < 1:1239736/57‑1 (MQ=255) cAGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGCCGGGCAtt < 1:1018152/57‑1 (MQ=255) aGCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGCCGGGCAtt < 1:2132318/56‑1 (MQ=255) gCCGTGGACCAACTGGCAGGAAGCACTGGATAACGTGCAGAAAGTGCCGGGCAtt < 1:487119/55‑1 (MQ=255) gAAGCACTGGATAACGTGCAGAAAGTGCCGGGCAtt < 1:2088036/36‑1 (MQ=25) aaGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGc > 1:2339102/1‑69 (MQ=255) aaGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGc > 1:947086/1‑69 (MQ=255) ggATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCtggt > 1:2234317/1‑66 (MQ=255) gATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCtg < 1:2529671/63‑1 (MQ=255) gATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCtg < 1:1663510/63‑1 (MQ=255) gATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCtg < 1:3132411/63‑1 (MQ=255) gATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCtg < 1:3324796/63‑1 (MQ=255) gATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCtg < 1:1112397/63‑1 (MQ=255) cACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGt > 1:2435963/1‑69 (MQ=255) cACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGt > 1:1851718/1‑69 (MQ=255) cACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGt > 1:1260152/1‑69 (MQ=255) cACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGt > 1:616479/1‑69 (MQ=255) cACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGT‑GAAAGTg > 1:14214/1‑69 (MQ=255) | GTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTG > minE/756771‑756893 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |