| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 760,905 | C→T | 39.0% | V26I (GTC→ATC) | ymfA ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 760,905 | 0 | C | T | 39.0% | 45.0 / 57.3 | 87 | V26I (GTC→ATC) | ymfA | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (29/24); new base T (20/14); total (49/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01 | |||||||||||
GGTATCAACAATGACGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTT > minE/760840‑760964 | ggTATCAACAATGACGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGacaac > 1:1781249/1‑69 (MQ=255)ggTATCAACAATGACGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGacaac > 1:2771751/1‑69 (MQ=255)ggTATCAACAATGACGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGacaac > 1:54468/1‑69 (MQ=255) aacaaTGACGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGa < 1:3137884/69‑1 (MQ=255) aacaaTGACGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGa < 1:970202/69‑1 (MQ=255) aacaaTGACGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGa < 1:1973984/69‑1 (MQ=255) caaTGACGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGaa < 1:1754103/68‑1 (MQ=255) caaTGACGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGaa < 1:1900879/68‑1 (MQ=255) caaTGACGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGaa < 1:2105497/68‑1 (MQ=255) aCGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGATCTGGTTATAAAAGACAACAACAGAAATGAgg < 1:2548116/69‑1 (MQ=255) aCGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAgt > 1:425696/1‑68 (MQ=255) aCGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAgg > 1:2512062/1‑69 (MQ=255) aCGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAgg > 1:2270114/1‑69 (MQ=255) aCGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAgg > 1:214914/1‑69 (MQ=255) aCGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAgg < 1:373526/69‑1 (MQ=255) aCGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAgg < 1:632649/69‑1 (MQ=255) aCGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAgg < 1:259726/69‑1 (MQ=255) aCGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAgg > 1:2824203/1‑69 (MQ=255) aCGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAgg > 1:1012484/1‑69 (MQ=255) aCGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAgg < 1:1708014/69‑1 (MQ=255) aCGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAgg > 1:853210/1‑69 (MQ=255) aCGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAgg < 1:1622480/69‑1 (MQ=255) aCGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAgg < 1:1720283/69‑1 (MQ=255) aCGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAgg > 1:1493153/1‑69 (MQ=255) aCGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAg > 1:2233059/1‑68 (MQ=255) gTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTAc < 1:2827708/68‑1 (MQ=255) gTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTAc < 1:2073648/68‑1 (MQ=255) tCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGa < 1:1255668/69‑1 (MQ=255) tCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGa < 1:326120/69‑1 (MQ=255) tCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGa < 1:2567284/69‑1 (MQ=255) tCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGa < 1:907896/69‑1 (MQ=255) tCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGa < 1:1484659/69‑1 (MQ=255) tCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGa < 1:2343063/69‑1 (MQ=255) tCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGa < 1:294047/69‑1 (MQ=255) tCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGa < 1:297928/69‑1 (MQ=255) tCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGa < 1:3177548/69‑1 (MQ=255) tCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGa < 1:755740/69‑1 (MQ=255) tCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGa < 1:1652331/69‑1 (MQ=255) tCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGa < 1:1916973/69‑1 (MQ=255) cgcgTCTTTGCTATAGGTGAACTGGTTATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGa < 1:2818738/68‑1 (MQ=255) cgcgTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGa < 1:2099993/45‑1 (MQ=255) cgcgTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGAcc > 1:2217852/1‑66 (MQ=255) cgcgTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGAcc > 1:1800411/1‑66 (MQ=255) cgcgTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGAcc > 1:810911/1‑66 (MQ=255) cgcgTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGAcc > 1:3046032/1‑66 (MQ=255) cgcgTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGAcc > 1:1701764/1‑66 (MQ=255) cgcgTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGAcc > 1:1466482/1‑66 (MQ=255) cgcgTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGAcc > 1:1698003/1‑66 (MQ=255) cgcgTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGAcc > 1:1642101/1‑66 (MQ=255) cgTCTTTGCTATAGGTGAACTGGTTATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGa < 1:3173417/66‑1 (MQ=255) tttGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGa < 1:719770/62‑1 (MQ=255) gTGAACTGGTTATAAAAGATAACAACAGAAATGAGGATCAGTACCAGAc > 1:2497700/1‑49 (MQ=255) gTGAACTGGTTATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAAGAAtata > 1:284785/1‑66 (MQ=255) gTGAACTGGTTATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAAGAATATAc > 1:2478266/1‑67 (MQ=255) gTGAACTGGTTATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAAGAATATAc > 1:2813336/1‑67 (MQ=255) gTGAACTGGTTATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAAGAATATAc > 1:2011592/1‑67 (MQ=255) gTGAACTGGTTATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAAGAATATAc > 1:1209451/1‑67 (MQ=255) gTGAACTGGTTATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAAGAATATAc > 1:1830459/1‑67 (MQ=255) gTGAACTGGTTATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAAGAATATAc > 1:90706/1‑67 (MQ=255) aaCTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTa > 1:3145005/1‑40 (MQ=255) aaCTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTAc > 1:963095/1‑41 (MQ=255) aaCTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTAc > 1:517193/1‑41 (MQ=255) aaCTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTAc > 1:1041162/1‑41 (MQ=255) aaCTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTAc > 1:525352/1‑41 (MQ=255) aaCTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTAc > 1:3086519/1‑41 (MQ=255) aaCTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTAc > 1:2093221/1‑41 (MQ=255) aaCTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTAc > 1:2149336/1‑41 (MQ=255) aaCTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTAc > 1:241652/1‑41 (MQ=255) cTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACgaaagaa < 1:1210041/69‑1 (MQ=255) cTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACgaaagaa > 1:1290738/1‑69 (MQ=255) cTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACgaaagaa > 1:828274/1‑69 (MQ=255) cTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACgaaagaa < 1:2527273/69‑1 (MQ=255) gTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAAc > 1:708029/1‑69 (MQ=255) ttATAAAAGATAACAACAGAAATGAGGATCAGTAc > 1:1921693/1‑35 (MQ=255) ttATAAAAGATAACAACAGAAATGAGGATCAGTACCATACCGATACCGAAGAATATACGAAAGAAAAc > 1:2651397/1‑68 (MQ=255) ttATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGAt > 1:1883052/1‑44 (MQ=255) ttATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAAGAATATACGAAAGAAAAc > 1:183070/1‑68 (MQ=255) ttATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAAGAATATACGAAAGAAAAc > 1:3026882/1‑68 (MQ=255) ttATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAAGAATATACGAAAGAAAAc > 1:925101/1‑68 (MQ=255) ttATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAAGAATATACGAAAGAAAAc > 1:1496993/1‑68 (MQ=255) ttATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAAGAATATACGAAAGAAAAc > 1:3216115/1‑68 (MQ=255) ttATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAAGAATATACGAAAGAAAAc > 1:3368655/1‑68 (MQ=255) ttATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAAGAATATACGAAAGAAAAc > 1:3279513/1‑68 (MQ=255) ttATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAAGAATATACGAAAGAAAAc > 1:2514642/1‑68 (MQ=255) ttATAAAAGATAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAAGAATATACGAAAGAAAAc > 1:415337/1‑68 (MQ=255) tataAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGTAATATACGAAAGAAAACtt < 1:3177710/69‑1 (MQ=255) tataAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACtt < 1:1820609/69‑1 (MQ=255) tataAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACtt < 1:322086/69‑1 (MQ=255) tataAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACtt < 1:1546582/69‑1 (MQ=255) tataAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACtt < 1:1278866/69‑1 (MQ=255) | GGTATCAACAATGACGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTT > minE/760840‑760964 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |