| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 767,700 | T→C | 57.5% | V444V (GTA→GTG) | phoQ ← | sensory histidine kinase in two‑compoent regulatory system with PhoP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 767,700 | 0 | T | C | 57.5% | 15.8 / 37.2 | 54 | V444V (GTA→GTG) | phoQ | sensory histidine kinase in two‑compoent regulatory system with PhoP |
| Reads supporting (aligned to +/- strand): ref base T (11/12); new base C (7/24); total (18/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.98e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01 | |||||||||||
CTCTCTCCGGCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTC > minE/767638‑767758 | ctctctCCGGCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCt > 1:292073/1‑69 (MQ=255)ctctctCCGGCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCt > 1:2842802/1‑69 (MQ=255)ctctctCCGGCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCt > 1:1481569/1‑69 (MQ=255) gCGACTATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCCACACATTGCCCAggg < 1:1936500/69‑1 (MQ=255) gCGACGATTTTACCCTGATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCCACACATTGCCCAggg < 1:3331286/69‑1 (MQ=255) gCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCCACACCTTGCCCAggg < 1:1852398/69‑1 (MQ=255) gCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCCACACCTTGCCCAggg < 1:2919484/69‑1 (MQ=255) gCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCCACACCTTGCCCAggg < 1:2930585/69‑1 (MQ=255) gCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCCACACCTTGCCCAggg < 1:2523373/69‑1 (MQ=255) gCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCCACACCTTGCCCAggg < 1:2384580/69‑1 (MQ=255) gCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCCACACCTTGCCCAggg < 1:3147515/69‑1 (MQ=255) gCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCCACACCTTGCCCAggg < 1:2119091/69‑1 (MQ=255) gCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCCACACCTTGCCCAggg < 1:1743112/69‑1 (MQ=255) gCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCCACACCTTGCCCAggg < 1:923320/69‑1 (MQ=255) gCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCCACACCTTGCCCAggg < 1:1179456/69‑1 (MQ=255) gCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCCACACCTT‑CCCAggg < 1:1455970/68‑1 (MQ=255) gCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCCACACATTGCCCAggg < 1:817188/69‑1 (MQ=255) gCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCCACACATTGCCCAggg < 1:167835/69‑1 (MQ=255) gCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCCACACATTGCCCAggg < 1:411460/69‑1 (MQ=255) gCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCCACACATTGCCCAggg < 1:1544310/69‑1 (MQ=255) gCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCCACACATTGCCCAggg < 1:1932430/69‑1 (MQ=255) gCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCCACACATTGCCCAggg < 1:1458078/69‑1 (MQ=255) gCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCCACACATTGCCCAggg < 1:647005/69‑1 (MQ=255) gCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCCACACATTGCCCAggg < 1:2289915/69‑1 (MQ=255) gCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCCACACATTGCCCAggg < 1:838607/69‑1 (MQ=255) cgacgaTTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCCACACATTGCCCAggg < 1:1531698/68‑1 (MQ=255) cgacgaTTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCCACACATTGCCCAggg < 1:2441146/68‑1 (MQ=255) cgaTTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTa < 1:3307582/69‑1 (MQ=255) cgaTTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTa < 1:2473306/69‑1 (MQ=255) cgaTTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTa < 1:2963296/69‑1 (MQ=255) cgaTTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTa < 1:1357135/69‑1 (MQ=255) cgaTTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTa < 1:558807/69‑1 (MQ=255) ttttACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAg < 1:1651353/69‑1 (MQ=255) ttttACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAg < 1:2547857/69‑1 (MQ=255) ttttACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAg < 1:2936818/69‑1 (MQ=255) cccTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATc < 1:596688/68‑1 (MQ=255) cccTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATc < 1:1089754/68‑1 (MQ=255) cccTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATc < 1:2867358/68‑1 (MQ=255) tGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTg > 1:1130891/1‑68 (MQ=255) tGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTg > 1:2287313/1‑68 (MQ=255) tGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTg > 1:1716255/1‑68 (MQ=255) ttCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGaa < 1:1279072/69‑1 (MQ=255) gCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACg > 1:1195914/1‑56 (MQ=255) gCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGAcc > 1:2295688/1‑69 (MQ=255) gCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGAc > 1:1074847/1‑68 (MQ=255) gCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGAc > 1:478938/1‑68 (MQ=255) gCTACCGCAAGCCCTACACCTTGCCCAGGGCGTAAAGTATc > 1:887848/1‑41 (MQ=255) ccGCCAGCCCCACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACctct > 1:1602950/1‑68 (MQ=255) ccGCCAGCCCCACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACctct > 1:2434673/1‑68 (MQ=255) ccGCCAGCCCCACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCtctc > 1:3361402/1‑69 (MQ=255) ccGCCAGCCCCACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCtctc > 1:1402122/1‑69 (MQ=255) ccGCCAGCCCCACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCtctc > 1:2156779/1‑69 (MQ=255) ccGCCAGCCCCACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCtctc > 1:2434050/1‑69 (MQ=255) ccGCCAGCCCCACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCtctc > 1:268217/1‑69 (MQ=255) | CTCTCTCCGGCGACGATTTTACCCTCATATTGCTCGGTGATTTCGCGGGCTACCGCCAGCCCTACACCTTGCCCAGGGCGTAAAGTATCAACCCGTTGACCACGGTCGAAAATGACCTCTC > minE/767638‑767758 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |