| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 768,263 | A→G | 71.8% | L257L (TTG→CTG) | phoQ ← | sensory histidine kinase in two‑compoent regulatory system with PhoP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 768,263 | 0 | A | G | 71.8% | 78.3 / 29.5 | 74 | L257L (TTG→CTG) | phoQ | sensory histidine kinase in two‑compoent regulatory system with PhoP |
| Reads supporting (aligned to +/- strand): ref base A (19/2); new base G (36/17); total (55/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.43e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
CTATGGGTCAGGTCGGTGAGCGTCGTACGGTATTTGTCGTAACGTTCGCGTTCACTTTTTAACAATCGGTTCAGGTTTCGTACCAGACTGGTCAGTTCTCGCGTTGTGGCTGGATTGA > minE/768199‑768316 | cTATGGGTCAGGTCGGTGAGCGTCGTACGGTATTTGTCGTAACGTTCGCGTTCACTTTTTAACAATCgg < 1:580871/69‑1 (MQ=255) tGGGTCAGGTCGGTGAGCGTCGTACGGTATTTGTCGTAACGTTCGCGTTCACTTTTTAACAATCGGTTc > 1:2791090/1‑69 (MQ=255) tGGGTCAGGTCGGTGAGCGTCGTACGGTATTTGTCGTAACGTTCGCGTTCACTTTTTAACAATCGGTTc > 1:1113015/1‑69 (MQ=255) tGGGTCAGGTCGGTGAGCGTCGTACGGTATTTGTCGTAACGTTCGCGTTCACTTTTTAACAATCGGTTc > 1:1154012/1‑69 (MQ=255) tGGGTCAGGTCGGTGAGCGTCGTACGGTATTTGTCGTAACGTTCGCGTTCACTTTTTAACAATCGGTTc > 1:1728029/1‑69 (MQ=255) tGGGTCAGGTCGGTGAGCGTCGTACGGTATTTGTCGTAACGTTCGCGTTCACTTTTTAACAATCGGTTc > 1:1907952/1‑69 (MQ=255) cGGTGAGCGTCGTACGGTATTTGTCGTAACGTTCGCGTTCACTTTTTAACAATCGGTTCAGGTTTCGt > 1:1529335/1‑68 (MQ=255) cGGTGAGCGTCGTACGGTATTTGTCGTAACGTTCGCGTTCACTTTTTAACAATCGGTTCAGGTTTCGt > 1:418225/1‑68 (MQ=255) cGGTGAGCGTCGTACGGTATTTGTCGTAACGTTCGCGTTCACTTTTTAACAATCGGTTCAGGTTTCGt > 1:388377/1‑68 (MQ=255) cGGTGAGCGTCGTACGGTATTTGTCGTAACGTTCGCGTTCACTTTTTAACAATCGGTTCAGGTTTCGt > 1:2335636/1‑68 (MQ=255) cGGTGAGCGTCGTACGGTATTTGTCGTAACGTTCGCGTTCACTTTTTAACAATCGGTTCAGGTTTCGt > 1:1280788/1‑68 (MQ=255) gtcgtACGATATTTGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg < 1:3025811/69‑1 (MQ=255) gtcgtACGATATTTGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg < 1:32631/69‑1 (MQ=255) gtcgtACGATATTTGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg < 1:3021683/69‑1 (MQ=255) gtcgtACGATATTTGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg < 1:3400868/69‑1 (MQ=255) gtcgtACGATATTTGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg < 1:1741757/69‑1 (MQ=255) gtcgtACGATATTTGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg < 1:2967579/69‑1 (MQ=255) gtcgtACGATATTTGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg < 1:290117/69‑1 (MQ=255) gtcgtACGATATTTGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg < 1:2780576/69‑1 (MQ=255) gtcgtACGATATTTGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg < 1:1501092/69‑1 (MQ=255) gtcgtACGATATTTGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg < 1:1324565/69‑1 (MQ=255) gtcgtACGATATTTGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg < 1:1377229/69‑1 (MQ=255) gtcgtACGATATTTGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg < 1:1398755/69‑1 (MQ=255) gtcgtACGATATTTGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg < 1:1399689/69‑1 (MQ=255) gtcgtACGATATTTGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg < 1:440871/69‑1 (MQ=255) atcgtACGATATTTGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg < 1:3274733/68‑1 (MQ=255) tcgtACGATATTTGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg < 1:1015313/68‑1 (MQ=255) gtACGGTATTTGTCGTAACGTTCGCGTTCACTTTTTAACAATCGGTTCAGGTTTCGTACCAGACTGGTc > 1:1931800/1‑69 (MQ=255) gtACGGTATTTGTCGTAACGTTCGCGTTCACTTTTTAACAATCGGTTCAGGTTTCGTACCAGACTGGTc > 1:2236270/1‑69 (MQ=255) gtACGGTATTTGTCGTAACGTTCGCGTTCACTTTTTAACAATCGGTTCAGGTTTCGTACCAGACTGGTc > 1:2840487/1‑69 (MQ=255) gtACGGTATTTGTCGTAACGTTCGCGTTCACTTTTTAACAATCGGTTCAGGTTTCGTACCAGACTGGTc > 1:852748/1‑69 (MQ=255) gtACGATATTTGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg < 1:1324081/66‑1 (MQ=255) tGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGTTTCAGGTTTCGTACCAGACTGGTCAGt > 1:98501/1‑62 (MQ=255) tGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGt > 1:1950816/1‑36 (MQ=255) tGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTGGTCAGTtctc > 1:1679537/1‑66 (MQ=255) tGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTGGTCAGTTCTcgcg > 1:1081556/1‑69 (MQ=255) tGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTGGTCAGTTCTcgcg > 1:397703/1‑69 (MQ=255) tGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTGGTCAGTTCTcgcg > 1:2986230/1‑69 (MQ=255) tGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTGGTCAGTTCTcgcg > 1:766349/1‑69 (MQ=255) tGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTGGTCAGTTCTcgcg > 1:648456/1‑69 (MQ=255) tGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTGGTCAGTTCTcgcg > 1:505916/1‑69 (MQ=255) tGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTGGTCAGTTCTcgcg > 1:185105/1‑69 (MQ=255) tGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTGGTCAGTTCTcgcg > 1:1132697/1‑69 (MQ=255) tGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTGGTCAGTTCTcgcg > 1:1230448/1‑69 (MQ=255) tGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTGGTCAGTTCTcgcg > 1:1462000/1‑69 (MQ=255) tGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTGGTCAGTTCTcgcg > 1:1712164/1‑69 (MQ=255) tGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTGGTCAGTTCTcgcg > 1:1991686/1‑69 (MQ=255) tGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTGGTCAGTTCTcgcg > 1:24105/1‑69 (MQ=255) tGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTGGTCAGTTCTcgcg > 1:2478364/1‑69 (MQ=255) tGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTGGTCAGTTCTcgcg > 1:2448549/1‑69 (MQ=255) tGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTGGTCAGTTCTcgcg > 1:2531815/1‑69 (MQ=255) tGTCGTAACGTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTGGTCAGTTCTcgc > 1:1824464/1‑68 (MQ=255) tGTCGTAACCTTCGCGTTCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTGGTCAGTTCTcgcg > 1:27548/1‑69 (MQ=255) aCGTTCGCGTTCACTTTTTAACAATCGGTTCAGGTTTCGTACCAGACTGGTCAGTTCTCGCGTTGTGGc > 1:2748588/1‑69 (MQ=255) aCGTTCGCGTTCACTTTTTAACAATCGGTTCAGGTTTCGTACCAGACTGGTCAGTTCTCGCGTTGTGGc > 1:1315496/1‑69 (MQ=255) aCGTTCGCGTTCACTTTTTAACAATCGGTTCAGGTTTCGTACCAGACTGGTCAGTTCTCGCGTTGTGGc > 1:134558/1‑69 (MQ=255) aCGTTCGCGTTCACTTTTTAACAATCGGTTCAGGTTTCGTACCAGACTGGTCAGTTCTCGCGTTGTGGc > 1:1765725/1‑69 (MQ=255) aCGTTCGCGTTCACTTTTTAACAATCGGTTCAGGTTTCGTACCAGACTGGTCAGTTCTCGCGTTGTGGc > 1:303136/1‑69 (MQ=255) ttCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg > 1:554095/1‑40 (MQ=255) ttCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg > 1:2298775/1‑40 (MQ=255) ttCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg > 1:806/1‑40 (MQ=255) ttCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg > 1:1294863/1‑40 (MQ=255) ttCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg > 1:696679/1‑40 (MQ=255) ttCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg > 1:692806/1‑40 (MQ=255) ttCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg > 1:2484845/1‑40 (MQ=255) ttCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg > 1:339250/1‑40 (MQ=255) ttCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg > 1:2823043/1‑40 (MQ=255) ttCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg > 1:1481504/1‑40 (MQ=255) ttCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg > 1:1983674/1‑40 (MQ=255) ttCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg > 1:3291783/1‑40 (MQ=255) ttCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg > 1:3389449/1‑40 (MQ=255) ttCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg > 1:386707/1‑40 (MQ=255) ttCACTTTTTAACAGTCGGTTCAGGTTTCGTACCAGACTg > 1:3401037/1‑40 (MQ=255) aaCAATCGGTTCAGGTTTCGTACCAGACTGGTCAGTTCTCGCGTTGTGGCTGGATTGa < 1:2713811/58‑1 (MQ=255) | CTATGGGTCAGGTCGGTGAGCGTCGTACGGTATTTGTCGTAACGTTCGCGTTCACTTTTTAACAATCGGTTCAGGTTTCGTACCAGACTGGTCAGTTCTCGCGTTGTGGCTGGATTGA > minE/768199‑768316 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |