| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 769,085 | G→C | 38.0% | P206P (CCC→CCG) | phoP ← | DNA‑binding response regulator in two‑component regulatory system with PhoQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 769,085 | 0 | G | C | 38.0% | 84.5 / 111.2 | 121 | P206P (CCC→CCG) | phoP | DNA‑binding response regulator in two‑component regulatory system with PhoQ |
| Reads supporting (aligned to +/- strand): ref base G (23/52); new base C (13/33); total (36/85) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.61e-01 | |||||||||||
Only 100 of 128 total aligned reads displayed.
TAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTT > minE/769020‑769145 | tAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGa < 1:92014/69‑1 (MQ=255)tAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGa < 1:2180462/69‑1 (MQ=255) aGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGaa > 1:3216073/1‑69 (MQ=255) aGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGaa > 1:841645/1‑69 (MQ=255) cAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAAtttt > 1:450414/1‑69 (MQ=255) cAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAAtttt > 1:2899487/1‑69 (MQ=255) cAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAAtttt > 1:66060/1‑69 (MQ=255) cAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAAtttt > 1:1334838/1‑69 (MQ=255) aCAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCa < 1:3292267/69‑1 (MQ=255) aCAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCa < 1:2550300/69‑1 (MQ=255) aCAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCa < 1:1914890/69‑1 (MQ=255) tAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTgcg < 1:2373325/62‑1 (MQ=255) tAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTgcg < 1:2245461/62‑1 (MQ=255) tAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTgcg < 1:52336/62‑1 (MQ=255) tAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTgcg < 1:2561219/62‑1 (MQ=255) tAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTgcg < 1:3232205/62‑1 (MQ=255) tAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTgcg < 1:1594639/62‑1 (MQ=255) aGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTc < 1:733260/69‑1 (MQ=255) aGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTc < 1:2129151/69‑1 (MQ=255) gCCCTGGCCGCGAACGGTGGTAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTc < 1:81381/68‑1 (MQ=255) gCCCTGGCCGCGAACGGTGGTAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTc < 1:1983105/68‑1 (MQ=255) ccTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTccc > 1:1805642/1‑68 (MQ=255) ccTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTccc > 1:71608/1‑68 (MQ=255) ccTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTccc > 1:3059380/1‑68 (MQ=255) ccTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTccc > 1:2838306/1‑68 (MQ=255) ccTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTccc > 1:1385228/1‑68 (MQ=255) ccTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTccc > 1:617186/1‑68 (MQ=255) tGGCCGCGAACGGTGGTAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATc > 1:1284288/1‑69 (MQ=255) tGGCCGCGAACGGTGGTAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATc > 1:1144749/1‑69 (MQ=255) tGGCCGCGAACGGTGGTAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATc > 1:403691/1‑69 (MQ=255) tGGCCGCGAACGGTGGTAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATc > 1:2204395/1‑69 (MQ=255) tGGCCGCGAACGGTGGTAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATc > 1:1255141/1‑69 (MQ=255) tGGCCGCGAACGGTGGTAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATc > 1:1296311/1‑69 (MQ=255) tGGCCGCGAACGGTGGTAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATc > 1:383309/1‑69 (MQ=255) cgcgAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAg < 1:2795117/67‑1 (MQ=255) cgcgAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAg < 1:3131844/67‑1 (MQ=255) cgcgAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAg < 1:963873/67‑1 (MQ=255) cgcgAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAg < 1:841226/67‑1 (MQ=255) cgcgAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAg < 1:760302/67‑1 (MQ=255) cgcgAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAg < 1:738869/67‑1 (MQ=255) cgcgAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAg < 1:615136/67‑1 (MQ=255) tggtAATCACTTCTTGGGGGTATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:582280/69‑1 (MQ=255) tggtAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:2080718/69‑1 (MQ=255) tggtAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:1087502/69‑1 (MQ=255) tggtAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:3025915/69‑1 (MQ=255) tggtAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:1472607/69‑1 (MQ=255) tggtAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:3336168/69‑1 (MQ=255) tggtAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:2632800/69‑1 (MQ=255) tggtAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:1003385/69‑1 (MQ=255) tggtAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:1888430/69‑1 (MQ=255) tggtAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:398558/69‑1 (MQ=255) tggtAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:220950/69‑1 (MQ=255) ggtAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:2569099/68‑1 (MQ=255) ggtAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:307436/68‑1 (MQ=255) ggtAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGt < 1:1852661/69‑1 (MQ=255) ggtAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGt < 1:3302993/69‑1 (MQ=255) ggtAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGt < 1:1856299/69‑1 (MQ=255) ggtAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGt < 1:2687543/69‑1 (MQ=255) ggtAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGt < 1:2358283/69‑1 (MQ=255) ggtAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGt < 1:1585121/69‑1 (MQ=255) ggtAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGt < 1:1938018/69‑1 (MQ=255) ggtAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGt < 1:2954127/69‑1 (MQ=255) gtAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAg < 1:2288295/56‑1 (MQ=255) aaTCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGTAt < 1:564564/68‑1 (MQ=255) aaTCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGTAt < 1:2610251/68‑1 (MQ=255) aaTCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGTAt < 1:879067/68‑1 (MQ=255) aaTCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGTAt < 1:3340984/68‑1 (MQ=255) aaTCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGTAt < 1:602270/68‑1 (MQ=255) aaTCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGTAt < 1:3030217/68‑1 (MQ=255) aaTCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGTAt < 1:1828664/68‑1 (MQ=255) aaTCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGTAt < 1:1761211/68‑1 (MQ=255) aaTCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGTAt < 1:1581640/68‑1 (MQ=255) aaTCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGTAt < 1:1973277/68‑1 (MQ=255) aaTCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGTAt < 1:2090318/68‑1 (MQ=255) aaTCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGTAt < 1:2094254/68‑1 (MQ=255) aaTCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGTAt < 1:115180/68‑1 (MQ=255) aaTCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGTAt < 1:2611443/68‑1 (MQ=255) aaTCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGTAt < 1:2750028/68‑1 (MQ=255) aTCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:1317235/64‑1 (MQ=255) aTCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:958041/64‑1 (MQ=255) aTCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:2669333/64‑1 (MQ=255) aTCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:2753986/64‑1 (MQ=255) aTCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:623681/64‑1 (MQ=255) cACTTCTTGGGGATATTGTGCCTGAATTTTTTTgcg < 1:1454273/36‑1 (MQ=255) cACTTCTTGGGGATATTGTGCCTGAATTTTTTTgcg < 1:2007051/36‑1 (MQ=255) cACTTCTTGGGGATATTGTGCCTGAATTTTTTTgcg < 1:1768823/36‑1 (MQ=255) aCTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGc > 1:57169/1‑67 (MQ=255) aCTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCtt > 1:3117542/1‑69 (MQ=255) aCTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCtt > 1:1030563/1‑69 (MQ=255) aCTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCtt > 1:2192798/1‑69 (MQ=255) ttcttGGGGATATTGTGCCTGAATTTTTTTGCGCAGCCGt > 1:2615277/1‑40 (MQ=255) ttcttGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGt > 1:1912938/1‑40 (MQ=255) ttcttGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGt > 1:240928/1‑40 (MQ=255) ttcttGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGt > 1:1107893/1‑40 (MQ=255) ggggATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAg < 1:1912549/43‑1 (MQ=255) ggggATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAg < 1:622286/43‑1 (MQ=255) ggggATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAg < 1:1931821/43‑1 (MQ=255) ggggATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAg < 1:2129696/43‑1 (MQ=255) ggggATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAg < 1:1429558/43‑1 (MQ=255) ggggATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAg < 1:2698657/43‑1 (MQ=255) | TAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTT > minE/769020‑769145 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |