| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 769,100 | A→T | 31.3% | I201I (ATT→ATA) | phoP ← | DNA‑binding response regulator in two‑component regulatory system with PhoQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 769,100 | 0 | A | T | 31.3% | 175.2 / 107.0 | 150 | I201I (ATT→ATA) | phoP | DNA‑binding response regulator in two‑component regulatory system with PhoQ |
| Reads supporting (aligned to +/- strand): ref base A (36/67); new base T (13/34); total (49/101) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
Only 100 of 152 total aligned reads displayed.
AGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCG > minE/769033‑769164 | aGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGaa > 1:841645/1‑69 (MQ=255)aGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGaa > 1:3216073/1‑69 (MQ=255) cAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAAtttt > 1:450414/1‑69 (MQ=255) cAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAAtttt > 1:2899487/1‑69 (MQ=255) aCAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCa < 1:2550300/69‑1 (MQ=255) aCAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCa < 1:1914890/69‑1 (MQ=255) aCAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCa < 1:3292267/69‑1 (MQ=255) tAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTgcg < 1:2838945/62‑1 (MQ=255) tAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTgcg < 1:52336/62‑1 (MQ=255) tAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTgcg < 1:3232205/62‑1 (MQ=255) tAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTgcg < 1:2373325/62‑1 (MQ=255) tAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTgcg < 1:2245461/62‑1 (MQ=255) tAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTgcg < 1:1594639/62‑1 (MQ=255) aGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTc < 1:1358350/69‑1 (MQ=255) gCCCTGGCCGCGAACGGTGGTAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTc < 1:1983105/68‑1 (MQ=255) gCCCTGGCCGCGAACGGTGGTAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTc < 1:81381/68‑1 (MQ=255) ccTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTccc > 1:71608/1‑68 (MQ=255) ccTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTccc > 1:1805642/1‑68 (MQ=255) ccTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTccc > 1:3059380/1‑68 (MQ=255) ccTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTccc > 1:617186/1‑68 (MQ=255) ccTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGAAGACGTccc > 1:1673144/1‑68 (MQ=255) tGGCCGCGAACGGTGGTAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATc > 1:1255141/1‑69 (MQ=255) tGGCCGCGAACGGTGGTAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATc > 1:2204395/1‑69 (MQ=255) tGGCCGCGAACGGTGGTAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATc > 1:725618/1‑69 (MQ=255) tGGCCGCGAACGGTGGTAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATc > 1:901994/1‑69 (MQ=255) tGGCCGCGAACGGTGGTAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATc > 1:3037774/1‑69 (MQ=255) tGGCCGCGAACGGTGGTAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATc > 1:383309/1‑69 (MQ=255) tGGCCGCGAACGGTGGTAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATc > 1:2581996/1‑69 (MQ=255) tGGCCGCGAACGGTGGTAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATc > 1:1144749/1‑69 (MQ=255) tGGCCGCGAACGGTGGTAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATc > 1:1296311/1‑69 (MQ=255) tGGCCGCGAACGGTGGTAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATc > 1:1284288/1‑69 (MQ=255) cgcgAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAg < 1:615136/67‑1 (MQ=255) cgcgAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAg < 1:963873/67‑1 (MQ=255) cgcgAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAg < 1:738869/67‑1 (MQ=255) cgcgAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAg < 1:2751953/67‑1 (MQ=255) cgcgAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAg < 1:2795117/67‑1 (MQ=255) cgcgAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAg < 1:760302/67‑1 (MQ=255) cgcgAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAg < 1:3131844/67‑1 (MQ=255) tggtAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:1003385/69‑1 (MQ=255) tggtAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:2080718/69‑1 (MQ=255) tggtAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:398558/69‑1 (MQ=255) tggtAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:2632800/69‑1 (MQ=255) tggtAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:3025915/69‑1 (MQ=255) tggtAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:3336168/69‑1 (MQ=255) tggtAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:2515226/69‑1 (MQ=255) ggtAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:2569099/68‑1 (MQ=255) ggtAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGt < 1:3302993/69‑1 (MQ=255) ggtAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGt < 1:601296/69‑1 (MQ=255) ggtAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGt < 1:1852661/69‑1 (MQ=255) ggtAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGt < 1:2358283/69‑1 (MQ=255) ggtAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGt < 1:2954127/69‑1 (MQ=255) ggtAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGt < 1:1856299/69‑1 (MQ=255) ggtAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGt < 1:1938018/69‑1 (MQ=255) ggtAATCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGt < 1:1932801/69‑1 (MQ=255) aaTCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGTAt < 1:228435/68‑1 (MQ=255) aaTCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGTAt < 1:115180/68‑1 (MQ=255) aaTCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGTAt < 1:879067/68‑1 (MQ=255) aaTCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGTAt < 1:1581640/68‑1 (MQ=255) aaTCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGTAt < 1:1761211/68‑1 (MQ=255) aaTCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGTAt < 1:1828664/68‑1 (MQ=255) aaTCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGTAt < 1:1889842/68‑1 (MQ=255) aaTCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGTAt < 1:2094254/68‑1 (MQ=255) aaTCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGTAt < 1:564564/68‑1 (MQ=255) aaTCACTTCTTGCGGATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCAGTACATCTATGGTAt < 1:2611443/68‑1 (MQ=255) aTCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:2753986/64‑1 (MQ=255) aTCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:623681/64‑1 (MQ=255) aTCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:2669333/64‑1 (MQ=255) aTCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:1317235/64‑1 (MQ=255) cACTTCTTGGGGATATTGTGCCTGAATTTTTTTgcg < 1:2007051/36‑1 (MQ=255) cACTTCTTGGGGATATTGTGCCTGAATTTTTTTgcg < 1:1520742/36‑1 (MQ=255) cACTTCTTGGGGATATTGTGCCTGAATTTTTTTgcg < 1:1454273/36‑1 (MQ=255) aCTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGc > 1:57169/1‑67 (MQ=255) aCTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCtt > 1:1030563/1‑69 (MQ=255) aCTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCtt > 1:3117542/1‑69 (MQ=255) aCTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCtt > 1:2192798/1‑69 (MQ=255) ttcttGGGGATATTGTGCCTGAATTTTTTTGCGCAGCCGt > 1:2615277/1‑40 (MQ=255) ttcttGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGt > 1:1912938/1‑40 (MQ=255) ttcttGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGt > 1:265210/1‑40 (MQ=255) ttcttGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGt > 1:1107893/1‑40 (MQ=255) ttGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgt < 1:2113070/56‑2 (MQ=255) ggggATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAg < 1:1429558/43‑1 (MQ=255) ggggATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAg < 1:2129696/43‑1 (MQ=255) ggggATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAg < 1:622286/43‑1 (MQ=255) ggggATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAg < 1:2698657/43‑1 (MQ=255) gATATTGTGCCTGTATTTTTTTGCGCAGACGTCCCATCATTACATCTATTGt < 1:1380967/52‑1 (MQ=255) ttGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGTCTTTCCCGCAGCTCCGc > 1:3408877/1‑69 (MQ=255) ttGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCTCAGCTCCGc < 1:1646820/69‑1 (MQ=255) ttGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGc > 1:600234/1‑69 (MQ=255) ttGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGc > 1:513557/1‑69 (MQ=255) ttGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGc > 1:2827719/1‑69 (MQ=255) ttGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGc > 1:811889/1‑69 (MQ=255) gtgCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:456465/45‑1 (MQ=255) gtgCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATgg < 1:3087107/45‑1 (MQ=255) gtgCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCAt > 1:843174/1‑69 (MQ=255) gtgCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCAt > 1:109477/1‑69 (MQ=255) gtgCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCAt > 1:2045499/1‑69 (MQ=255) gtgCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCAt > 1:219883/1‑69 (MQ=255) gtgCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCAt > 1:2707670/1‑69 (MQ=255) ccTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCg < 1:2138633/69‑1 (MQ=255) ccTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCg < 1:2150735/69‑1 (MQ=255) | AGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCG > minE/769033‑769164 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |