| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 771,789 | G→C | 73.6% | A33G (GCC→GGC) | hflD ← | predicted lysogenization regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 771,789 | 0 | G | C | 73.6% | 105.7 / 39.0 | 72 | A33G (GCC→GGC) | hflD | predicted lysogenization regulator |
| Reads supporting (aligned to +/- strand): ref base G (15/4); new base C (25/28); total (40/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.98e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
CCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATA > minE/771727‑771845 | ccGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATcac < 1:2301421/69‑1 (MQ=255)ccGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATcac < 1:620509/69‑1 (MQ=255) gCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGc < 1:2787063/51‑1 (MQ=255) gCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGc < 1:1377802/51‑1 (MQ=255) cATATCGATAATACTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCTTGGTGAGc > 1:1736013/1‑63 (MQ=255) cATATCGATAATACTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCt > 1:1749207/1‑56 (MQ=255) cATATCGATAATACTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAg > 1:135050/1‑62 (MQ=255) cATATCGATAATACTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGc > 1:3107672/1‑63 (MQ=255) cATATCGATAATACTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGc > 1:234068/1‑63 (MQ=255) cATATCGATAATACTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGc > 1:2405538/1‑63 (MQ=255) cATATCGATAATACTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGc > 1:2423222/1‑63 (MQ=255) cATATCGATAATACTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGc > 1:1002380/1‑63 (MQ=255) cATATCGATAATACTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGc > 1:2952230/1‑63 (MQ=255) cATATCGATAATACTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGc > 1:2760514/1‑63 (MQ=255) cATATCGATAATACTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGc > 1:3274721/1‑63 (MQ=255) cATATCGATAATACTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGc > 1:3337125/1‑63 (MQ=255) cATATCGATAATACTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGc > 1:3353593/1‑63 (MQ=255) cATATCGATAATACTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGc > 1:391869/1‑63 (MQ=255) cATATCGATAATACTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGc > 1:512751/1‑63 (MQ=255) cATATCGATAATACTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGc > 1:58239/1‑63 (MQ=255) cATATCGATAATACTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGc > 1:929439/1‑63 (MQ=255) cATATCGATAATACTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGc > 1:980777/1‑63 (MQ=255) cATATCGATAATACTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGc > 1:1876396/1‑63 (MQ=255) cATATCGATAATACTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGc > 1:1125419/1‑63 (MQ=255) cATATCGATAATACTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGc > 1:1153984/1‑63 (MQ=255) cATATCGATAATACTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGc > 1:130145/1‑63 (MQ=255) cATATCGATAATACTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGc > 1:1307113/1‑63 (MQ=255) cATATCGATAATACTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGCTGAGc > 1:1431157/1‑63 (MQ=255) cATATCGATAATACTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCATGGTGAGc > 1:3023800/1‑63 (MQ=255) cAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGttgttg > 1:1608655/1‑67 (MQ=255) cAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGc > 1:1059923/1‑68 (MQ=255) aataCTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGCGAGttgttg < 1:1946235/63‑1 (MQ=255) aataCTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGCGAGttgttg < 1:492641/63‑1 (MQ=255) aataCTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGCGAGttgttg < 1:470607/63‑1 (MQ=255) aataCTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGCGAGttgttg < 1:439517/63‑1 (MQ=255) aataCTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGCGAGttgttg < 1:214207/63‑1 (MQ=255) aataCTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGCGAGttgttg < 1:3386159/63‑1 (MQ=255) aataCTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGCGAGttgttg < 1:1361693/63‑1 (MQ=255) aataCTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGCGAGttgttg < 1:337789/63‑1 (MQ=255) aataCTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGCGAGttgttg < 1:220296/63‑1 (MQ=255) aataCTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGCGAGttgttg < 1:2220759/63‑1 (MQ=255) aataCTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGCGAGttgttg < 1:767501/63‑1 (MQ=255) aataCTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGCGAGttgttg < 1:135819/63‑1 (MQ=255) aataCTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGCGAGttgttg < 1:3139306/63‑1 (MQ=255) aataCTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGCGAGttgttg < 1:3136849/63‑1 (MQ=255) aataCTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGCGAGttgttg < 1:769905/63‑1 (MQ=255) aataCTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGCGAGttgttg < 1:3106153/63‑1 (MQ=255) aataCTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGCGAGttgttg < 1:1051518/63‑1 (MQ=255) aataCTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGCGAGttgttg < 1:889123/63‑1 (MQ=255) aataCTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGCGAGttgttg < 1:2497705/63‑1 (MQ=255) aataCTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGCGAGttgttg < 1:2583964/63‑1 (MQ=255) aataCTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGCGAGttgttg < 1:2687503/63‑1 (MQ=255) aataCTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGCGAGttgttg < 1:1611481/63‑1 (MQ=255) aataCTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGCGAGttgttg < 1:2818087/63‑1 (MQ=255) aataCTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGCGAGttgttg < 1:812860/63‑1 (MQ=255) aataCTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGCGAGttgttg < 1:2898983/63‑1 (MQ=255) aataCTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGCGAGttgttg < 1:3065821/63‑1 (MQ=255) aataCTGTTGAGTGAGACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGCGAGttgttg < 1:3098164/63‑1 (MQ=255) aCTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGc > 1:1862840/1‑68 (MQ=255) aCTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGc > 1:1851574/1‑68 (MQ=255) aCTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCg > 1:1020040/1‑69 (MQ=255) aCTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCg > 1:1902296/1‑69 (MQ=255) aCTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCg > 1:692321/1‑69 (MQ=255) aCTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCg > 1:611496/1‑69 (MQ=255) aCTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCg > 1:3298613/1‑69 (MQ=255) aCTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCg > 1:2882147/1‑69 (MQ=255) aCTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCACCTGGTGAGCGAGTTGTTGCACCAGGCg > 1:1799775/1‑69 (MQ=255) agtgagACGTGTAGCGCATCGCCATCACAATGCCCCTGGTGAGCGAGttgttg < 1:1081009/53‑1 (MQ=255) tgagACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGCGCGAGTTGTTGCACCAGGCGTGCCGACt > 1:504031/1‑68 (MQ=255) tgagACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACt > 1:312459/1‑68 (MQ=255) tgagACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACt > 1:2238260/1‑68 (MQ=255) tgtAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATa > 1:1650378/1‑69 (MQ=255) | CCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATA > minE/771727‑771845 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |