| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 771,851 | C→A | 45.0% | L12L (CTG→CTT) | hflD ← | predicted lysogenization regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 771,851 | 0 | C | A | 45.0% | 18.9 / 57.6 | 60 | L12L (CTG→CTT) | hflD | predicted lysogenization regulator |
| Reads supporting (aligned to +/- strand): ref base C (15/18); new base A (17/10); total (32/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.06e-01 | |||||||||||
ACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTG > minE/771794‑771902 | acaATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGt < 1:1147361/69‑1 (MQ=255)acaATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGt < 1:2559814/69‑1 (MQ=255)acaATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGt > 1:2631659/1‑69 (MQ=255)acaATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGt < 1:21982/69‑1 (MQ=255)acaATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGt < 1:67754/69‑1 (MQ=255)acaATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGt > 1:2747566/1‑69 (MQ=255)acaATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGt > 1:2751565/1‑69 (MQ=255)acaATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGt > 1:2832653/1‑69 (MQ=255)acaATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAAGGt < 1:3097660/69‑1 (MQ=255) caATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGt < 1:104128/68‑1 (MQ=255) caATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCAAGGGCGAGGGt > 1:2071441/1‑68 (MQ=255) caATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCAAGGGCGAGGGTg > 1:424670/1‑69 (MQ=255) caATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCAAGGGCGAGGGTg > 1:2574068/1‑69 (MQ=255) caATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCAAGGGCGAGGGTg > 1:3174518/1‑69 (MQ=255) caATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCAAGGGCGAGGGTg > 1:3249058/1‑69 (MQ=255) caATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCAAGGGCGAGGGTg > 1:3322064/1‑69 (MQ=255) caATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCAAGGGCGAGGGTg > 1:3036791/1‑69 (MQ=255) caATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCAAGGGCGAGGGTg > 1:2886106/1‑69 (MQ=255) caATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCAAGGGCGAGGGTg > 1:293926/1‑69 (MQ=255) caATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCAAGGGCGAGGGTg > 1:1565995/1‑69 (MQ=255) caATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCAAGGGCGAGGGTg > 1:756100/1‑69 (MQ=255) caATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCAAGGGCGAGGGTg > 1:150413/1‑69 (MQ=255) caATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCAAGGGCGAGGGTg > 1:1499827/1‑69 (MQ=255) caATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCAAGGGCGAGGGTg > 1:834381/1‑69 (MQ=255) caATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCAAGGGCGAGGGTg > 1:135663/1‑69 (MQ=255) caATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCAAGGGCGAGGGTg > 1:877590/1‑69 (MQ=255) caATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCAAGGGCGAGGGTg > 1:2946812/1‑69 (MQ=255) aTGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGAt > 1:3011728/1‑69 (MQ=255) aTGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGAt > 1:2031011/1‑69 (MQ=255) aTGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGAt > 1:3299755/1‑69 (MQ=255) aTGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGAt > 1:2027144/1‑69 (MQ=255) aTGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGAt > 1:1903117/1‑69 (MQ=255) aTGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGAt > 1:436128/1‑69 (MQ=255) aTGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGAt > 1:1060170/1‑69 (MQ=255) aTGCCCATGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGAt > 1:440343/1‑69 (MQ=255) gCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGt > 1:400646/1‑69 (MQ=255) ggCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGt < 1:1981563/37‑1 (MQ=255) gCGTGCCGACTGACAAATACCGGCAAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGc < 1:3429390/69‑1 (MQ=255) gCGTGCCGACTGACAAATACCGGCAAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGc < 1:955088/69‑1 (MQ=255) gCGTGCCGACTGACAAATACCGGCAAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGc < 1:1172227/69‑1 (MQ=255) gCGTGCCGACTGACAAATACCGGCAAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGc < 1:2418370/69‑1 (MQ=255) gCGTGCCGACTGACAAATACCGGCAAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGc < 1:1518435/69‑1 (MQ=255) gCGTGCCGACTGACAAATACCGGCAAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGc < 1:2299500/69‑1 (MQ=255) gCGTGCCGACTGACAAATACCGGCAAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGc < 1:2215107/69‑1 (MQ=255) gCGTGCCGACTGACAAATACCGGCAAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGc < 1:1654124/69‑1 (MQ=255) gCGTGCCGACTGACAAATACCGGCAAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGc < 1:1729703/69‑1 (MQ=255) cGTGCCGACTGACAAATACCGGCAAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGc < 1:2868235/68‑1 (MQ=255) tGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTc < 1:1748404/69‑1 (MQ=255) tGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTc < 1:943450/69‑1 (MQ=255) tGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTc < 1:1310937/69‑1 (MQ=255) tGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTc < 1:2337661/69‑1 (MQ=255) tGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTc < 1:3234953/69‑1 (MQ=255) tGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTc < 1:2670370/69‑1 (MQ=255) ggCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTc < 1:2861554/68‑1 (MQ=255) ccGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCt > 1:2100390/1‑69 (MQ=255) ccGACTGACAAATACCGGCCAGGGCGAGGGTGATGTAATAGTAATTCTTTGCCACGTTCACTGCTTCCt > 1:576063/1‑69 (MQ=255) gACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTg < 1:189420/69‑1 (MQ=255) gACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTg < 1:1380930/69‑1 (MQ=255) ccGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGtt < 1:1746687/44‑1 (MQ=255) ccGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGtt < 1:693014/44‑1 (MQ=255) | ACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTG > minE/771794‑771902 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |