| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 773,262 | G→C | 66.2% | T94T (ACC→ACG) | ymfB ← | bifunctional thiamin pyrimidine pyrophosphate hydrolase and thiamin pyrophosphate hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 773,262 | 0 | G | C | 66.2% | 45.9 / 34.3 | 49 | T94T (ACC→ACG) | ymfB | bifunctional thiamin pyrimidine pyrophosphate hydrolase and thiamin pyrophosphate hydrolase |
| Reads supporting (aligned to +/- strand): ref base G (10/6); new base C (18/14); total (29/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 | |||||||||||
CCTGTAAAATTTCTTCGGCGCTGACCCAACGGCAGCAGTCGATATCGCTGTCATGAGGCTGGGTCGGGCATATTTGCTCAAGCTCAATGGCAAAGAGGAAACGCAAAAACGGCGTTTTATCTGG > minE/773201‑773324 | ccTGTAAAATTTCTTCGGCGCTGACCCAACGGCAGCAGTCGATATCGCTGTCATGAGGCTGGGTCggg > 1:865386/1‑68 (MQ=255) aaaTTTCTTCGGCGCTGACCCAACGGCAGCAGTCGATATCGCTGTCATGAGGCTGGGTCGGGCATAttt > 1:2369576/1‑69 (MQ=255) aaaTTTCTTCGGCGCTGACCCAACGGCAGCAGTCGATATCGCTGTCATGAGGCTGGGTCGGGCATAttt > 1:1177546/1‑69 (MQ=255) aaaTTTCTTCGGCGCTGACCCAACGGCAGCAGTCGATATCGCTGTCATGAGGCTGGGTCGGGCATAttt > 1:742889/1‑69 (MQ=255) aaaTTTCTTCGGCGCTGACCCAACGGCAGCAGTCGATATCGCTGTCATGAGGCTGGGTCGGGCATAttt > 1:742162/1‑69 (MQ=255) aaaTTTCTTCGGCGCTGACCCAACGGCAGCAGTCGATATCGCTGTCATGAGGCTGGGTCGGGCATAttt > 1:1777017/1‑69 (MQ=255) aaaTTTCTTCGGCGCTGACCCAACGGCAGCAGTCGATATCGCTGTCATGAGGCTGGGTCGGGCATAtt > 1:2207512/1‑68 (MQ=255) cGGCGCTGACCCAACGGCAGCAGTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTtctca < 1:3293766/65‑1 (MQ=255) cGGCGCTGACCCAACGGCAGCAGTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTgctca < 1:1701137/65‑1 (MQ=255) cGGCGCTGACCCAACGGCAGCAGTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTgctca < 1:2494680/65‑1 (MQ=255) cGGCGCTGACCCAACGGCAGCAGTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTgctca < 1:2750325/65‑1 (MQ=255) cGGCGCTGACCCAACGGCAGCAGTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTgctca < 1:2754399/65‑1 (MQ=255) cGGCGCTGACCCAACGGCAGCAGTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTgctca < 1:335960/65‑1 (MQ=255) cGGCGCTGACCCAACGGCAGCAGTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTgctca < 1:1584202/65‑1 (MQ=255) cGGCGCTGACCCAACGGCAGCAGTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTgctca < 1:630850/65‑1 (MQ=255) cGGCGCTGACCCAACGGCAGCAGTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTgctca < 1:2878828/65‑1 (MQ=255) cGGCGCTGACCCAACGGCAGCAGTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTgctca < 1:733677/65‑1 (MQ=255) cGGCGCTGACCCAACGGCAGCAGTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTgctca < 1:786174/65‑1 (MQ=255) cGGCGCTGACCCAACGGCAGCAGTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTgatca < 1:2873422/65‑1 (MQ=255) gcgcTGACCCAACGGCAGCAGTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTgctca < 1:2286789/63‑1 (MQ=255) aCGGCAGCAGTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTgctca < 1:1941613/52‑1 (MQ=255) cagTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTgctcaagctcaa > 1:3139162/1‑52 (MQ=255) cagTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTGCTCAAGCTCAATGGCAAAGAGGAAAc > 1:910562/1‑67 (MQ=255) cagTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTGCTCAAGCTCAATGGCAAAGAGGAAACg > 1:321959/1‑68 (MQ=255) cagTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTGCTCAAGCTCAATGGCAAAGAGGAAACg > 1:2959853/1‑68 (MQ=255) cagTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTGCTCAAGCTCAATGGCAAAGAGGAAACg > 1:3318071/1‑68 (MQ=255) cagTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTGCTCAAGCTCAATGGCAAAGAGGAAACg > 1:3331435/1‑68 (MQ=255) cagTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTGCTCAAGCTCAATGGCAAAGAGGAAACg > 1:685514/1‑68 (MQ=255) cagTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTGCTCAAGCTCAATGGCAAAGAGGAAACg > 1:2618549/1‑68 (MQ=255) cagTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTGCTCAAGCTCAATGGCAAAGAGGAAACg > 1:1762690/1‑68 (MQ=255) cagTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTGCTCAAGCTCAATGGCAAAGAGGAAACg > 1:1116552/1‑68 (MQ=255) cagTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTGCTCAAGCTCAATGGCAAAGAGGAAACg > 1:1383486/1‑68 (MQ=255) cagTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTGCTCAAGCTCAATGGCAAAGAGGAAACg > 1:141898/1‑68 (MQ=255) cagTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTGCTCAAGCTCAATGGCAAAGAGGAAACg > 1:2817581/1‑68 (MQ=255) cagTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTGCTCAAGCTCAATGGCAAAGAGGAAACg > 1:1938840/1‑68 (MQ=255) cagTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTGCTCAAGCTCAATGGCAAAGAGGAAACg > 1:209013/1‑68 (MQ=255) cagTCGATATCGCTGTCATGAGGCTGCGTCGGGCATATTTGCTCAAGCTCAATGGCAAAGAGGAAACg > 1:2758542/1‑68 (MQ=255) cagTCGATATCGCTGTCATGAGGCTGAGTCGGGCATATTTGCTCAAGCTCAATGGCAAAGAGGAAACg > 1:1407909/1‑68 (MQ=255) cagTCGATATCGCTGTCATGAGCCTGCGTCGGGCATATTTGCTCAAGCTCAATGGCAAAGAGGAAACg > 1:1097253/1‑68 (MQ=255) cagTCGACATCGCTGTCATGAGGCTGCGTCGGGCATATTTGCTCAAGCTCAATGGCAAAGAGGAAACg > 1:1857664/1‑68 (MQ=255) gCTGTCATGAGGCTGGGTCGGGCATATTTGCTCAAGCTCAATGGCAAAGAGGAAACGCAAAAACGGCGt > 1:2215806/1‑69 (MQ=255) aGGCTGGGTCGGGCATATTTGCTCAAGCTCAATGGCAAAGAGGAAACGCAAAAACGGCGTTTTATCTgg > 1:2019321/1‑69 (MQ=255) aGGCTGGGTCGGGCATATTTGCTCAAGCTCAATGGCAAAGAGGAAACGCAAAAACGGCGTTTTATCTgg < 1:318579/69‑1 (MQ=255) aGGCTGGGTCGGGCATATTTGCTCAAGCTCAATGGCAAAGAGGAAACGCAAAAACGGCGTTTTATCTgg < 1:419197/69‑1 (MQ=255) aGGCTGGGTCGGGCATATTTGCTCAAGCTCAATGGCAAAGAGGAAACGCAAAAACGGCGTTTTATCTgg < 1:1548059/69‑1 (MQ=255) aGGCTGGGTCGGGCATATTTGCTCAAGCTCAATGGCAAAGAGGAAACGCAAAAACGGCGTTTTATCTgg < 1:2896164/69‑1 (MQ=255) aGGCTGGGTCGGGCATATTTGCTCAAGCTCAATGGCAAAGAGGAAACGCAAAAACGGCGTTTTATCTgg < 1:1334319/69‑1 (MQ=255) aGGCTGGGTCGGGCATATTTGCTCAAGCTCAATGGCAAAGAGGAAACGCAAAAACGGCGTTTTATCTgg < 1:119405/69‑1 (MQ=255) aGGCTGGGTCGGGCATATTTGCTCAAGCTCAATGGCAAAGAGGAAACGCAAAAACGGCGTTTTATCTg > 1:1272177/1‑68 (MQ=255) | CCTGTAAAATTTCTTCGGCGCTGACCCAACGGCAGCAGTCGATATCGCTGTCATGAGGCTGGGTCGGGCATATTTGCTCAAGCTCAATGGCAAAGAGGAAACGCAAAAACGGCGTTTTATCTGG > minE/773201‑773324 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |