| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 774,341 | C→T | 68.2% | intergenic (‑135/‑37) | ymfC ← / → icd | 23S rRNA pseudouridine synthase/isocitrate dehydrogenase, specific for NADP+ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 774,341 | 0 | C | T | 68.2% | 36.1 / 21.3 | 47 | intergenic (‑135/‑37) | ymfC/icd | 23S rRNA pseudouridine synthase/isocitrate dehydrogenase, specific for NADP+ |
| Reads supporting (aligned to +/- strand): ref base C (8/7); new base T (17/15); total (25/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTG > minE/774283‑774396 | aaaaaaTTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGTAGACGAGCaa < 1:2084544/69‑1 (MQ=255)aaaaaaTTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGTAGACGAGCaa < 1:2832753/69‑1 (MQ=255)aaaaaaTTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGTAGACGAGCaa < 1:2957401/69‑1 (MQ=255)aaaaaaTTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGTAGACGAGCaa < 1:1272238/69‑1 (MQ=255)aaaaaaTTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGTAGACGAGCaa < 1:2196238/69‑1 (MQ=255)aaaaaaTTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGTAGACGAGCaa < 1:2147241/69‑1 (MQ=255)aaaaaaTTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGTAGACGAGCaa < 1:1885019/69‑1 (MQ=255)aaaaaaTTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGTAGACGAGCaa < 1:3296927/69‑1 (MQ=255)aaaaaaTTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGTAGACGAGCaa < 1:1502208/69‑1 (MQ=255)aaaaaaTTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGTAGACGAGCaa < 1:978819/69‑1 (MQ=255)aaaaaaTTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGTAGACGAGCaa < 1:903885/69‑1 (MQ=255)aaaaaaTTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGTAGACGAGCaa < 1:3386129/69‑1 (MQ=255)aaaaaaTTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGTAGACGAGCaa < 1:838381/69‑1 (MQ=255)aaaaaaTTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGTAGACGAGCaa < 1:389100/69‑1 (MQ=255) aTCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGTGCTCGAAgg < 1:2442944/69‑1 (MQ=255) gTATCGCAGGACGCAAACGCATATGCAACGTGGTGGTAGACGAGCaa < 1:920547/47‑1 (MQ=255) tCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTg < 1:3043225/69‑1 (MQ=255) tCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTg < 1:2777183/69‑1 (MQ=255) cGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGaa > 1:2057/1‑68 (MQ=255) cGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGaa > 1:342819/1‑68 (MQ=255) aaaCGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTg < 1:2267949/58‑1 (MQ=255) tatGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAagtagt < 1:3064395/69‑1 (MQ=255) tatGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAagtagt < 1:249219/69‑1 (MQ=255) atGCAACGTGGTGGTAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGaaagta > 1:2647771/1‑61 (MQ=255) atGCAACGTGGTGGTAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGaaagta > 1:3009353/1‑61 (MQ=255) atGCAACGTGGTGGTAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGaaagta > 1:3241251/1‑61 (MQ=255) atGCAACGTGGTGGTAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGaaagta > 1:2998203/1‑61 (MQ=255) atGCAACGTGGTGGTAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGaaagta > 1:2990388/1‑61 (MQ=255) atGCAACGTGGTGGTAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGaaagta > 1:2264219/1‑61 (MQ=255) atGCAACGTGGTGGTAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGaaagta > 1:2170340/1‑61 (MQ=255) atGCAACGTGGTGGTAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGaaagta > 1:2120528/1‑61 (MQ=255) atGCAACGTGGTGGTAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGaaagta > 1:1282939/1‑61 (MQ=255) atGCAACGTGGTGGTAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGaaagta > 1:1306010/1‑61 (MQ=255) atGCAACGTGGTGGTAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGaaagta > 1:1754180/1‑61 (MQ=255) atGCAACGTGGTGGTAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGaaagta > 1:1739204/1‑61 (MQ=255) atGCAACGTGGTGGTAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGaaagta > 1:1358412/1‑61 (MQ=255) atGCAACGTGGTGGTAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGaaagta > 1:1706255/1‑61 (MQ=255) atGCAACGTGGTGGTAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGaaagta > 1:1601073/1‑61 (MQ=255) atGCAACGTGGTGGTAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGaaagta > 1:1576951/1‑61 (MQ=255) atGCAACGTGGTGGTAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGaaag > 1:2594977/1‑59 (MQ=255) atGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAagtagt < 1:2794227/68‑1 (MQ=255) tGCAACGTGTTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAgttg > 1:2723372/1‑69 (MQ=255) tGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAgttg > 1:501949/1‑69 (MQ=255) tGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAgttg > 1:3418106/1‑69 (MQ=255) tGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAgttg > 1:2002188/1‑69 (MQ=255) tGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAgttg > 1:976929/1‑69 (MQ=255) tGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAgttg > 1:2072995/1‑69 (MQ=255) cAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTg < 1:641079/36‑1 (MQ=255) | AAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTG > minE/774283‑774396 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |