| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 76,662 | G→A | 45.7% | P170P (CCG→CCA) | ftsI → | transpeptidase involved in septal peptidoglycan synthesis |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 76,662 | 0 | G | A | 45.7% | 17.6 / 76.8 | 94 | P170P (CCG→CCA) | ftsI | transpeptidase involved in septal peptidoglycan synthesis |
| Reads supporting (aligned to +/- strand): ref base G (15/36); new base A (22/21); total (37/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.64e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGG > minE/76600‑76724 | tACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCgg > 1:3095836/1‑68 (MQ=255)tACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCgg > 1:1631488/1‑68 (MQ=255)tACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCgg > 1:553399/1‑68 (MQ=255)tACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCgg > 1:535651/1‑68 (MQ=255)tACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCgg > 1:1283810/1‑68 (MQ=255)tACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCgg > 1:1002303/1‑68 (MQ=255)tACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCgg > 1:3362911/1‑68 (MQ=255) cAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGt > 1:2673180/1‑69 (MQ=255) aaaaCTGAAACTGCCGGGGATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGAc < 1:1015712/69‑1 (MQ=255) aaaaCTGAAACTGCCGGGGATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGAc < 1:1196489/69‑1 (MQ=255) aaaaCTGAAACTGCCGGGGATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGAc < 1:934593/69‑1 (MQ=255) aaactgaaactgCCGGGGATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGAc < 1:1680330/68‑1 (MQ=255) actgCCGGGGATTCATCTGCGTTAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCt < 1:780613/69‑1 (MQ=255) actgCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCt < 1:2641038/69‑1 (MQ=255) actgCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCt < 1:1711784/69‑1 (MQ=255) actgCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCt < 1:428820/69‑1 (MQ=255) actgCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCt < 1:1389752/69‑1 (MQ=255) actgCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCt < 1:1308214/69‑1 (MQ=255) actgCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCt < 1:1819949/69‑1 (MQ=255) actgCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCt < 1:1831536/69‑1 (MQ=255) actgCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCt < 1:2013892/69‑1 (MQ=255) actgCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCt < 1:918616/69‑1 (MQ=255) ctgCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCt < 1:3015986/68‑1 (MQ=255) ctgCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCt < 1:1107494/68‑1 (MQ=255) ggATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCTCATCGGCt < 1:2937030/69‑1 (MQ=255) ggATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCTCATCGGCt < 1:2381278/69‑1 (MQ=255) ggATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCTCATCGGCt < 1:2120674/69‑1 (MQ=255) ggATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCTCATCGGCt < 1:1170104/69‑1 (MQ=255) ggATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCTCATCGGCt < 1:2608426/69‑1 (MQ=255) ggATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCTCATCGGCt < 1:844886/69‑1 (MQ=255) ggATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCTCATCGGCt < 1:384056/69‑1 (MQ=255) ggATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCTCATCGGCt < 1:2825082/69‑1 (MQ=255) ggATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCTCATCGGCt < 1:2868887/69‑1 (MQ=255) gATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCtt < 1:448353/69‑1 (MQ=255) gATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCtt < 1:3393720/69‑1 (MQ=255) gATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCtt < 1:2435747/69‑1 (MQ=255) ttCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCTCATCGGCttt > 1:994081/1‑68 (MQ=255) ttCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCTCATCGGCttt > 1:3376729/1‑68 (MQ=255) ttCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCTCATCGGCttt > 1:870030/1‑68 (MQ=255) ttCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCTCATCGGCttt > 1:286462/1‑68 (MQ=255) ttCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCTCATCGGCttt > 1:2593161/1‑68 (MQ=255) ttCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCTCATCGGCttt > 1:2433302/1‑68 (MQ=255) ttCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCTCATCGGCttt > 1:695189/1‑68 (MQ=255) ttCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCTCATCGGCttt > 1:2321556/1‑68 (MQ=255) ttCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCTCATCGGCttt > 1:1586463/1‑68 (MQ=255) ttCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCTCATCGGCttt > 1:1580895/1‑68 (MQ=255) ttCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCTCATCGGCttt > 1:56160/1‑68 (MQ=255) ttCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCTCATCGGCttt > 1:139449/1‑68 (MQ=255) ttCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCTCATCGGCttt > 1:793880/1‑68 (MQ=255) ttCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCTCATCGGCttt > 1:1875093/1‑68 (MQ=255) ttCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCTCATCGGCttt > 1:2357503/1‑68 (MQ=255) ttCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCTCATCGGCttt > 1:1961626/1‑68 (MQ=255) ttCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCTCATCGGCttt > 1:2126888/1‑68 (MQ=255) ttCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCTCATCGGCttt > 1:377119/1‑68 (MQ=255) ttCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACCGCTCACCTCATCGGCttt > 1:374175/1‑68 (MQ=255) ttCATCTGCGTGAAGAATCTCGCCGTTATTATACATCCGGCGAAGTGACCGCTCACCTCATCGGCttt > 1:2077632/1‑68 (MQ=255) ttCATCTGCGTGAAGAATCTCGCCGTTATTATACATCCGGCGAAGTGACCGCTCACCTCATCGGCttt > 1:2445032/1‑68 (MQ=255) ttCATCTGCGTGAAGAATCTCGCCGTTAATATCCATCCGGCGAAGTGACCGCTCACCTCATCGGCttt > 1:865401/1‑68 (MQ=255) aTCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACt < 1:2953729/68‑1 (MQ=255) tCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTa < 1:1113879/68‑1 (MQ=255) tCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTa < 1:1998388/68‑1 (MQ=255) tGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTACCg < 1:1466386/65‑1 (MQ=255) tGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACg < 1:249047/65‑1 (MQ=255) tGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACg < 1:1282612/65‑1 (MQ=255) tGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACg < 1:1494070/65‑1 (MQ=255) tGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACg < 1:2701702/65‑1 (MQ=255) tGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTc > 1:1004820/1‑67 (MQ=255) tGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTc < 1:169959/67‑1 (MQ=255) tGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTc > 1:1107666/1‑67 (MQ=255) tGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTc < 1:2675384/67‑1 (MQ=255) tGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTc < 1:402147/67‑1 (MQ=255) aaGAGTCTTGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATa > 1:817909/1‑69 (MQ=255) aaGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATa > 1:1130336/1‑69 (MQ=255) aaGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATa > 1:1774297/1‑69 (MQ=255) aaGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATa > 1:976760/1‑69 (MQ=255) aaGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATa > 1:3111406/1‑69 (MQ=255) agTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTc < 1:171839/69‑1 (MQ=255) agTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTc < 1:1600629/69‑1 (MQ=255) agTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTc < 1:3104636/69‑1 (MQ=255) cGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAggg < 1:2287579/66‑1 (MQ=255) cGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAggg < 1:2130973/66‑1 (MQ=255) cGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAggg < 1:1789328/66‑1 (MQ=255) cGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAggg < 1:2496160/66‑1 (MQ=255) cGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAggg < 1:3269866/66‑1 (MQ=255) cGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAggg < 1:3061179/66‑1 (MQ=255) cGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAggg < 1:477988/66‑1 (MQ=255) cGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAggg < 1:3258919/66‑1 (MQ=255) cGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAggg < 1:1108104/66‑1 (MQ=255) cGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGAtt < 1:1727681/69‑1 (MQ=255) cGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGAtt < 1:1644161/69‑1 (MQ=255) cGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGAtt < 1:3280127/69‑1 (MQ=255) cGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGAtt < 1:3180286/69‑1 (MQ=255) cGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGAtt < 1:2736009/69‑1 (MQ=255) tATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAggg < 1:1150894/68‑1 (MQ=255) tccgtccgGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTc < 1:1225050/53‑1 (MQ=255) tccgtccgGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTc < 1:95700/53‑1 (MQ=255) | TACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGG > minE/76600‑76724 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |