| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 774,641 | T→C | 44.1% | T88T (ACT→ACC) | icd → | isocitrate dehydrogenase, specific for NADP+ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 774,641 | 0 | T | C | 44.1% | 20.2 / 83.6 | 102 | T88T (ACT→ACC) | icd | isocitrate dehydrogenase, specific for NADP+ |
| Reads supporting (aligned to +/- strand): ref base T (17/40); new base C (17/28); total (34/68) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 103 total aligned reads displayed.
TGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTG > minE/774574‑774702 | tggaAATTTACACCGGTGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAActc > 1:1764923/1‑69 (MQ=255) gTGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTg < 1:840989/69‑1 (MQ=255) gTGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTg < 1:2271234/69‑1 (MQ=255) gTGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTg < 1:2755233/69‑1 (MQ=255) gTGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTg < 1:304676/69‑1 (MQ=255) gTGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTg < 1:391909/69‑1 (MQ=255) gTGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTg < 1:451381/69‑1 (MQ=255) gTGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTg < 1:1034891/69‑1 (MQ=255) gTGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTAGATCTGATTCGTg < 1:1090528/69‑1 (MQ=255) gAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGaa < 1:2072356/69‑1 (MQ=255) gAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGaa < 1:1158297/69‑1 (MQ=255) gAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGaa < 1:380916/69‑1 (MQ=255) gAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGaa < 1:2430258/69‑1 (MQ=255) gAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGaa < 1:3271522/69‑1 (MQ=255) gAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGaa < 1:729760/69‑1 (MQ=255) gAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGaa < 1:3042623/69‑1 (MQ=255) tCCACACAGGTTTATGTTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATcgc < 1:1671207/69‑1 (MQ=255) tCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATcgc < 1:2777298/69‑1 (MQ=255) ggTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAgg < 1:2291998/68‑1 (MQ=255) ggTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAgg < 1:566532/68‑1 (MQ=255) ggTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAgg < 1:1341437/68‑1 (MQ=255) ggTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGGTGCCATTAAAgg < 1:203942/68‑1 (MQ=255) tCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATcgc < 1:152679/52‑1 (MQ=255) cAGGACGTCTGGTTGCCTGCTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCATTa < 1:331201/61‑1 (MQ=255) cAGGACGTCTGGTTGCCTGCTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCATTa < 1:560348/61‑1 (MQ=255) cAGGACGTCTGGTTGCCTGCTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCATTa < 1:445245/61‑1 (MQ=255) cAGGACGTCTGGTTGCCTGCTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCATTa < 1:2081878/61‑1 (MQ=255) cAGGACGTCTGGTTGCCTGCTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCATTa < 1:2214392/61‑1 (MQ=255) cAGGACGTCTGGTTGCCTGCTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCATTa < 1:1006540/61‑1 (MQ=255) cAGGACGTCTGGTTGCCTGCTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCATTa < 1:2275256/61‑1 (MQ=255) cAGGACGTCTGGTTGCCTGCTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCATTa < 1:3331348/61‑1 (MQ=255) cAGGACGTCTGGTTGCCTGCTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCATTa < 1:2409008/61‑1 (MQ=255) cAGGACGTCTGGTTGCCTGCTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCATTa < 1:487661/61‑1 (MQ=255) cAGGACGTCTGGTTGCCTGCTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCATTa < 1:3228515/61‑1 (MQ=255) cAGGACGTCTGGTTGCCTGCTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCATTa < 1:2436657/61‑1 (MQ=255) cAGGACGTCTGGTTGCCTGCTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCATTa < 1:2446126/61‑1 (MQ=255) cAGGACGTCTGGTTGCCTGCTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCATTa < 1:3195971/61‑1 (MQ=255) cAGGACGTCTGGTTGCCTGCTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCATTa < 1:2516400/61‑1 (MQ=255) cAGGACGTCTGGTTGCCTGCTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCATTa < 1:2706837/61‑1 (MQ=255) cAGGACGTCTGGTTGCCTGCTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCATTa < 1:31685/61‑1 (MQ=255) cAGGACGTCTGGTTGCCTGCTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCATTa < 1:1006441/61‑1 (MQ=255) cAGGACGTCTGGTTGCCTGCTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCATTa < 1:1141993/61‑1 (MQ=255) cAGGACGTCTGGTTGCCTGCTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCATTa < 1:1171120/61‑1 (MQ=255) cAGGACGTCTGGTTGCCTGCTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCATTa < 1:118791/61‑1 (MQ=255) cAGGACGTCTGGTTGCCTGCTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCATTa < 1:1258607/61‑1 (MQ=255) cAGGACGTCTGGTTGCCTGCTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCATTa < 1:1715895/61‑1 (MQ=255) cAGGACGTCTGGTTGCCTGCTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCATTa < 1:1690069/61‑1 (MQ=255) cAGGACGTCTGGTTGCCTGCTGAAACCCTTGATCAGATTCGTGAATATCGCGTTGCCATTa < 1:2015490/61‑1 (MQ=255) aGGACGTCTGGTTGCCTGCTGAAACCCTTGATCTGATTCGTTAATATCGCGTTGCCATTa < 1:1227985/60‑1 (MQ=255) aGGACGTCTGGTTGCCTGCTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCATTa < 1:1267898/60‑1 (MQ=255) aGGACGTCTGGTTGCCTGCTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCATTa < 1:1411639/60‑1 (MQ=255) gACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCt < 1:2206321/68‑1 (MQ=255) gACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCt < 1:888564/68‑1 (MQ=255) gACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCt < 1:598373/68‑1 (MQ=255) aCGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTcc > 1:3021391/1‑64 (MQ=255) aCGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCt < 1:3404054/67‑1 (MQ=255) aCGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTg > 1:2678189/1‑68 (MQ=255) aCGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTg > 1:1553517/1‑68 (MQ=255) aCGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTg > 1:52270/1‑68 (MQ=255) aCGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTg > 1:2285144/1‑68 (MQ=255) aCGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTg > 1:3424263/1‑68 (MQ=255) aCGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTg > 1:326617/1‑68 (MQ=255) cGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGAc < 1:2327733/69‑1 (MQ=255) cGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGAc < 1:961841/69‑1 (MQ=255) cGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGAc < 1:834420/69‑1 (MQ=255) cGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGAc < 1:64856/69‑1 (MQ=255) cGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGAc < 1:1032315/69‑1 (MQ=255) cGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGAc < 1:1145470/69‑1 (MQ=255) cGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGAc < 1:1848264/69‑1 (MQ=255) cGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGAc < 1:3261122/69‑1 (MQ=255) cGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGAc < 1:1304672/69‑1 (MQ=255) cGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGAc < 1:180815/69‑1 (MQ=255) cGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGAc < 1:3012652/69‑1 (MQ=255) cGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTAAc < 1:155597/69‑1 (MQ=255) gcctgcTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCAtt > 1:147702/1‑46 (MQ=255) gcctgcTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCAtt > 1:2586611/1‑46 (MQ=255) gcctgcTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCAtt > 1:200609/1‑46 (MQ=255) gcctgcTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCAtt > 1:750181/1‑46 (MQ=255) gcctgcTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCAtt > 1:1009225/1‑46 (MQ=255) gcctgcTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCAtt > 1:2001772/1‑46 (MQ=255) gcctgcTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCAtt > 1:2734333/1‑46 (MQ=255) gcctgcTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCAtt > 1:2761150/1‑46 (MQ=255) gcctgcTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCAtt > 1:518299/1‑46 (MQ=255) gcctgcTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCAtt > 1:2462275/1‑46 (MQ=255) gcctgcTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCAtt > 1:1459590/1‑46 (MQ=255) gcctgcTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCAtt > 1:1171628/1‑46 (MQ=255) gcctgcTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCAtt > 1:2479924/1‑46 (MQ=255) gcctgcTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCAtt > 1:1241709/1‑46 (MQ=255) gcctgcTGAAACCCTTGATCTGATTCGTGAATATCGCGTTGCCAtt > 1:1837770/1‑46 (MQ=255) gcctgcTGAAACCCTTGATCTGATTCGTGAATATCGCGTTCCCAtt > 1:609341/1‑46 (MQ=255) ctgAAACTCTTGATCTGATTCGTGAATATCGCGTTTCCATTAAAGGTCCGCt > 1:2643773/1‑52 (MQ=255) ctgAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCTGTtggtg > 1:3234860/1‑69 (MQ=255) ctgAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTtggtg > 1:3303811/1‑69 (MQ=255) ctgAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTtggtg > 1:653321/1‑69 (MQ=255) ctgAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTtggtg > 1:1656120/1‑69 (MQ=255) ctgAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTtggtg > 1:3195917/1‑69 (MQ=255) ctgAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTtggtg > 1:1108973/1‑69 (MQ=255) ctgAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTtggtg > 1:2004587/1‑69 (MQ=255) ctgAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTtggtg > 1:1143338/1‑69 (MQ=255) ctgAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTtggt > 1:415129/1‑68 (MQ=255) | TGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTG > minE/774574‑774702 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |