| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 775,538 | A→G | 68.8% | K387K (AAA→AAG) | icd → | isocitrate dehydrogenase, specific for NADP+ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 775,538 | 0 | A | G | 68.8% | 111.7 / 57.1 | 118 | K387K (AAA→AAG) | icd | isocitrate dehydrogenase, specific for NADP+ |
| Reads supporting (aligned to +/- strand): ref base A (29/8); new base G (62/19); total (91/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
Only 100 of 126 total aligned reads displayed.
TGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGT > minE/775477‑775604 | tGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAACCCGTaa > 1:2680222/1‑69 (MQ=255)tGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTaa > 1:772292/1‑69 (MQ=255)tGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTaa > 1:1201333/1‑69 (MQ=255)tGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTaa > 1:288849/1‑69 (MQ=255)tGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTaa > 1:2454129/1‑69 (MQ=255)tGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTaa > 1:2335969/1‑69 (MQ=255) tGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTAt < 1:963609/69‑1 (MQ=255) ggACTGAAGCCGCAGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCGAAGACCGTAACCTATg > 1:2585782/1‑69 (MQ=255) ggACTGAAGCCGCAGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCGAAGACCGTAACCTATg > 1:992935/1‑69 (MQ=255) ggACTGAAGCCGCAGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCGAAGACCGTAACCTATg > 1:632127/1‑69 (MQ=255) ggACTGAAGCCGCAGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCGAAGACCGTAACCTATg > 1:579090/1‑69 (MQ=255) ggACTGAAGCCGCAGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCGAAGACCGTAACCTATg > 1:3241630/1‑69 (MQ=255) ggACTGAAGCCGCAGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCGAAGACCGTAACCTATg > 1:2530065/1‑69 (MQ=255) ggACTGAAGCCGCAGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCGAAGACCGTAACCTATg > 1:2273056/1‑69 (MQ=255) ggACTGAAGCCGCAGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCGAAGACCGTAACCTATg > 1:2266607/1‑69 (MQ=255) ggACTGAAGCCGCAGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCGAAGACCGTAACCTATg > 1:1531874/1‑69 (MQ=255) ggACTGAAGCCGCAGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCGAAGACCGTAACCTATg > 1:151717/1‑69 (MQ=255) ggACTGAAGCCGCAGACCTGATTGTTAAAGGTATGGAAGGCGCAATAAATGCGAAGACCGTAACCTATg > 1:3417884/1‑69 (MQ=255) gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAGTCAACGCGAAGACCGTAACCTATGACTTCGAACGt > 1:1396900/1‑69 (MQ=255) gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAGACCGTAACCTATGACTTCGAACg > 1:2346191/1‑68 (MQ=255) gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAGACCGTAACCTATGACTTCGAACGt > 1:360874/1‑69 (MQ=255) gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAGACCGTAACCTATGACTTCGAACGt > 1:3427716/1‑69 (MQ=255) gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAGACCGTAACCTATGACTTCGAACGt > 1:2132907/1‑69 (MQ=255) gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAGACCGTAACCTATGACTTCGAACGt > 1:3228760/1‑69 (MQ=255) gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAGACCGTAACCTATGACTTCGAACGt > 1:1397277/1‑69 (MQ=255) gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAGACCGTAACCTATGACTTCGAACGt > 1:2963093/1‑69 (MQ=255) gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAGACCGTAACCTATGACTTCGAACGt > 1:2425631/1‑69 (MQ=255) gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAGACCGTAACCTATGACTTCGAACGt > 1:1111701/1‑69 (MQ=255) gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAGACCGTAACCTATGACTTCGAACGt > 1:1388905/1‑69 (MQ=255) gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAGACCGTAACCTATGACTTCGAACGt > 1:132555/1‑69 (MQ=255) gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAGACCGTAACCTATGACTTCGAACGt > 1:905341/1‑69 (MQ=255) ggTATGGAAGGCGCAATCAACGCGAAAACTGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa > 1:2001127/1‑69 (MQ=255) ggTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa > 1:2992324/1‑69 (MQ=255) ggTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa > 1:103650/1‑69 (MQ=255) ggTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa > 1:2089885/1‑69 (MQ=255) ggTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaa < 1:413514/68‑1 (MQ=255) ggTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaa < 1:215109/68‑1 (MQ=255) ggTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaa < 1:1288346/68‑1 (MQ=255) ggTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaa < 1:1002253/68‑1 (MQ=255) ggTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaa < 1:66876/68‑1 (MQ=255) gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaa < 1:251616/62‑1 (MQ=255) gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAActgct > 1:2774260/1‑68 (MQ=255) gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAActgct > 1:432009/1‑68 (MQ=255) cgcAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAAt > 1:166868/1‑68 (MQ=255) cgcAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATg > 1:2528833/1‑69 (MQ=255) cgcAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATg > 1:1975014/1‑69 (MQ=255) gcAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATg > 1:1159998/1‑68 (MQ=255) tCAACGCGAAGACCGTAACCTATGACTTCGAACTTCTGATGGAAGGCGCTAAACTGCTGAAATGTTCag > 1:817912/1‑69 (MQ=255) tCAACGCGAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAACTGCTGAAATGTTCag > 1:617799/1‑69 (MQ=255) tCAACGCGAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAACTGCTGAAATGTTCag > 1:293957/1‑69 (MQ=255) tCAACGCGAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAACTGCTGAAATGTTCag > 1:2885405/1‑69 (MQ=255) tCAACGCGAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAACTGCTGAAATGTTCag > 1:571311/1‑69 (MQ=255) tCAACGCGAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAACTGCTGAAATGTTCag > 1:2879782/1‑69 (MQ=255) tCAACGCGAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAACTGCTGAAATGTTCag > 1:2786476/1‑69 (MQ=255) tCAACGCGAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAACTGCTGAAATGTTCag > 1:3222826/1‑69 (MQ=255) tCAACGCGAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAACTGCTGAAATGTTCag > 1:1428818/1‑69 (MQ=255) tCAACGCGAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAACTGCTGAAATGTTCag > 1:1749670/1‑69 (MQ=255) tCAACGCGAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAACTGCTGAAATGTTCag > 1:1856523/1‑69 (MQ=255) tCAACGCGAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAACTGCTGAAATGTTCag > 1:2482237/1‑69 (MQ=255) tCAACGCGAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAACTGCTGAAATGTTCag > 1:1431610/1‑69 (MQ=255) tCAACCCGAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTa > 1:418862/1‑51 (MQ=255) cAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCaga > 1:157687/1‑69 (MQ=255) cAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCaga > 1:663349/1‑69 (MQ=255) cAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCaga > 1:2289865/1‑69 (MQ=255) aatgcgAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCagag < 1:328662/66‑1 (MQ=255) aatgcgAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCagag < 1:649822/66‑1 (MQ=255) aatgcgAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCagag < 1:2021907/66‑1 (MQ=255) aatgcgAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCagag < 1:412918/66‑1 (MQ=255) aatgcgAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCagag < 1:2167968/66‑1 (MQ=255) aatgcgAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCagag < 1:2178148/66‑1 (MQ=255) aatgcgAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCagag < 1:3386772/66‑1 (MQ=255) aatgcgAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCagag < 1:3299891/66‑1 (MQ=255) aatgcgAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCagag < 1:2662660/66‑1 (MQ=255) aatgcgAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCagag < 1:307168/66‑1 (MQ=255) aatgcgAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCagag < 1:2333005/66‑1 (MQ=255) aaCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCagag > 1:3304954/1‑69 (MQ=255) aaCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCagag > 1:1719292/1‑69 (MQ=255) aaCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCagag > 1:1683526/1‑69 (MQ=255) atgcgAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCagag < 1:2992589/66‑1 (MQ=255) atgcgAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCagag < 1:2895741/66‑1 (MQ=255) cgcgAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTaa > 1:715857/1‑48 (MQ=38) cgcgAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAAGTGCTGAAATGTTCAGAGtt > 1:1091785/1‑69 (MQ=255) cgcgAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAACTGCTGAAATGTTCAGAGtt > 1:1165290/1‑69 (MQ=255) cgcgAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAACTGCTGAAATGTTCAGAGtt > 1:349641/1‑69 (MQ=255) cgcgAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAACTGCTGAAATGTTCAGAGtt > 1:681535/1‑69 (MQ=255) cgcgAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAACTGCTGAAATGTTCAGAGtt > 1:1572673/1‑69 (MQ=255) cgcgAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAACTGCTGAAATGTTCAGAGtt > 1:458608/1‑69 (MQ=255) cgcgAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAACTGCTGAAATGTTCAGAGtt > 1:2202576/1‑69 (MQ=255) cgcgAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAACTGCTGAAATGTTCAGAGtt > 1:3202750/1‑69 (MQ=255) cgcgAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAACTGCTGAAATGTTCAGAGtt > 1:2330927/1‑69 (MQ=255) cgcgAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAACTGCTGAAATGTTCAGAGtt > 1:2468269/1‑69 (MQ=255) cgcgAAGACCGTAACCTATGACTTCGAACGTCTGATGGAAGGCGCTAAACTGCTGAAATGTTCAGAGtt > 1:2806041/1‑69 (MQ=255) aaaaCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGt < 1:1885426/69‑1 (MQ=255) aaaaCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGt < 1:462317/69‑1 (MQ=255) aaaaCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGt < 1:1934197/69‑1 (MQ=255) aaaaCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGt < 1:1202619/69‑1 (MQ=255) aaaaCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGt < 1:1287802/69‑1 (MQ=255) aaaaCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGt < 1:2858904/69‑1 (MQ=255) aaaCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGt < 1:496910/68‑1 (MQ=255) aaaCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGt < 1:2671653/68‑1 (MQ=255) | TGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGT > minE/775477‑775604 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |