| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 780,059 | G→T | 36.2% | L55L (CTG→CTT) | ycgL → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 780,059 | 0 | G | T | 36.2% | 77.5 / 56.9 | 80 | L55L (CTG→CTT) | ycgL | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (16/35); new base T (13/16); total (29/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTA > minE/780007‑780108 | ttCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGc > 1:320078/1‑66 (MQ=255)ttCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGc > 1:3192518/1‑66 (MQ=255)ttCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGc > 1:1785771/1‑66 (MQ=255)ttCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGc > 1:2688190/1‑66 (MQ=255)ttCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGc > 1:360571/1‑66 (MQ=255) tGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGgaaga < 1:393838/68‑1 (MQ=255) tGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGgaaga < 1:1533219/68‑1 (MQ=255) tGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGgaaga < 1:2920274/68‑1 (MQ=255) gAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGgaaga < 1:963900/67‑1 (MQ=255) gAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGgaaga < 1:1704694/67‑1 (MQ=255) gAACTGATGAAAGGTTTTGGTCAGCCTCGGTTAGCGATGATTCTTCCGCTGGATGGGCGGAAGAAAc < 1:2978832/67‑1 (MQ=255) gAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTTCCGCTGGATGGGCGGAAGAAAc < 1:2786820/67‑1 (MQ=255) gAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTTCCGCTGGATGGGCGGAAGAAAc < 1:1176247/67‑1 (MQ=255) gAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTTCCGCTGGATGGGCGGAAGAAAc < 1:404821/67‑1 (MQ=255) gAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTTCCGCTGGATGGGCGGAAGAAAc < 1:2261387/67‑1 (MQ=255) gAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTTCCGCTGGATGGGCGGAAGAAAc < 1:2164138/67‑1 (MQ=255) gAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTTCCGCTGGATGGGCGGAAGAAAc < 1:772752/67‑1 (MQ=255) gAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTTCCGCTGGATGGGCGGAAGAAAc < 1:831094/67‑1 (MQ=255) gAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTTCCGCTGGATGGGCGGAAGAAAc < 1:2000046/67‑1 (MQ=255) gAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTTCCGCTGGATGGGCGGAAGAAAc < 1:1722095/67‑1 (MQ=255) gAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTTCCGCTGGATGGGCGGAAGAAAc < 1:948223/67‑1 (MQ=255) gAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTTCCGCTGGATGGGCGGAAGAAAc < 1:1662009/67‑1 (MQ=255) gAACTGATCAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTTCCGCTGGATGGGCGGAAGAAAc < 1:1858466/67‑1 (MQ=255) aaCTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTTCCGCTGGATGGGCGGAAGAAAc < 1:2787151/66‑1 (MQ=255) cTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTc < 1:2245492/69‑1 (MQ=255) cTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTc < 1:250878/69‑1 (MQ=255) cTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTc < 1:2458780/69‑1 (MQ=255) cTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTc < 1:2387096/69‑1 (MQ=255) cTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTc < 1:2998128/69‑1 (MQ=255) cTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTc < 1:333829/69‑1 (MQ=255) cTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTc < 1:2054059/69‑1 (MQ=255) tgatgaAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTc < 1:1649650/68‑1 (MQ=255) tgatgaAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTc < 1:67156/68‑1 (MQ=255) tgatgaAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTc < 1:629721/68‑1 (MQ=255) tgatgaAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTc < 1:569006/68‑1 (MQ=255) tttGGTCAGCCTCAGTTAGCGATGATTCTTCCGCTGGATGGGc > 1:1264929/1‑43 (MQ=255) tttGGTCAGCCTCAGTTAGCGATGATTCTTCCGCTGGATGGGc > 1:2528919/1‑43 (MQ=255) tttGGTCAGCCTCAGTTAGCGATGATTCTTCCGCTGGATGGGc > 1:1224496/1‑43 (MQ=255) tttGGTCAGCCTCAGTTAGCGATGATTCTTCCGCTGGATGGGc > 1:1206930/1‑43 (MQ=255) tttGGTCAGCCTCAGTTAGCGATGATTCTTCCGCTGGATGGGc > 1:1677863/1‑43 (MQ=255) tttGGTCAGCCTCAGTTAGCGATGATTCTTCCGCTGGATGGGc > 1:2104937/1‑43 (MQ=255) tttGGTCAGCCTCAGTTAGCGATGATTCTTCCGCTGGATGGGc > 1:860141/1‑43 (MQ=255) tttGGTCAGCCTCAGTTAGCGATGATTCTTCCGCTGGATGGGc > 1:2314430/1‑43 (MQ=255) tttGGTCAGCCTCAGTTAGCGATGATTCTTCCGCTGGATGGGc > 1:1743260/1‑43 (MQ=255) tttGGTCAGCCTCAGTTAGCGATGATTCTTCCGCTGGATGGGc > 1:2244999/1‑43 (MQ=255) tttGGTCAGCCTCAGTTAGCGATGATTCTTCCGCTGGATGGGc > 1:412362/1‑43 (MQ=255) tttGGTCAGCCTCAGTTAGCGATGATTCTTCCGCTGGATGGGc > 1:684095/1‑43 (MQ=255) tttGGTCAGCCTCAGTTAGCGATGATTCTTCCGCTGGATGGGc > 1:175763/1‑43 (MQ=255) ttGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAAt < 1:1037554/59‑1 (MQ=255) ttGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAAt < 1:3417994/59‑1 (MQ=255) ttGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAAt < 1:2163857/59‑1 (MQ=255) ttGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAAt < 1:742392/59‑1 (MQ=255) ggTCAGCCTCAGTTAGCGATGATTCTTCCGCTGGATGGGCGGAAGAAAc < 1:2651508/49‑1 (MQ=255) gTCAGCCTCAGTTAGCGATGATTCTTCCGCTGGATGGGCGGAAGAAAc < 1:3079647/48‑1 (MQ=255) aGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGaaaa > 1:3422973/1‑67 (MQ=255) aGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAg > 1:530306/1‑68 (MQ=255) aGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAg > 1:994184/1‑68 (MQ=255) aGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAg > 1:1867905/1‑68 (MQ=255) aGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAg > 1:237192/1‑68 (MQ=255) cTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTa < 1:2454915/69‑1 (MQ=255) cTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTa < 1:1370015/69‑1 (MQ=255) cTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTa < 1:1590877/69‑1 (MQ=255) cTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTa < 1:869429/69‑1 (MQ=255) cTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTa < 1:2112776/69‑1 (MQ=255) cTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTa < 1:2119105/69‑1 (MQ=255) cTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTa < 1:2129169/69‑1 (MQ=255) cTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTa < 1:2413215/69‑1 (MQ=255) cTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTa < 1:2622906/69‑1 (MQ=255) cTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTa < 1:2855000/69‑1 (MQ=255) cTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTa < 1:3382594/69‑1 (MQ=255) aGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGcc > 1:3316118/1‑46 (MQ=255) aGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGcc > 1:2631613/1‑46 (MQ=255) aGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGcc > 1:2686270/1‑46 (MQ=255) aGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGcc < 1:222026/46‑1 (MQ=255) aGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGcc > 1:2697058/1‑46 (MQ=255) aGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGcc < 1:2752342/46‑1 (MQ=255) aGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGcc < 1:3426945/46‑1 (MQ=255) aGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGcc > 1:1914635/1‑46 (MQ=255) aGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGcc < 1:2826623/46‑1 (MQ=255) aGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGcc > 1:310622/1‑46 (MQ=255) | TTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTA > minE/780007‑780108 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |