| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 11,087 | C→G | 22.1% | R90R (CGG→CGC) A86A (GCC→GCG) |
yaaW ← htgA → |
conserved hypothetical protein hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 11,087 | 0 | C | G | 22.1% | 168.6 / 40.7 | 95 | R90R (CGG→CGC) A86A (GCC→GCG) | yaaW htgA | conserved hypothetical protein hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (52/22); new base G (8/13); total (60/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.02e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCT > minE/11020‑11153 | tttCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGcct > 1:1463419/1‑68 (MQ=255)tttCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGccc > 1:623581/1‑69 (MQ=255) tctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGa > 1:3344729/1‑54 (MQ=255) tctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGa > 1:2300272/1‑54 (MQ=255) tctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGa > 1:2261246/1‑54 (MQ=255) tctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGa > 1:2208120/1‑54 (MQ=255) tctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGa > 1:3434077/1‑54 (MQ=255) tctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGa > 1:878052/1‑54 (MQ=255) tctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGa > 1:3160892/1‑54 (MQ=255) tctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGa > 1:3189057/1‑54 (MQ=255) tctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCgtgt > 1:201185/1‑69 (MQ=255) tctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCgtgt > 1:2446105/1‑69 (MQ=255) tctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCgtgt > 1:2768854/1‑69 (MQ=255) tctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCgtgt > 1:2195901/1‑69 (MQ=255) tctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCgtgt > 1:347534/1‑69 (MQ=255) tctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCgtgt > 1:2178091/1‑69 (MQ=255) tctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCgtgt > 1:3008372/1‑69 (MQ=255) tctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCgtgt > 1:1477391/1‑69 (MQ=255) tctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCgtgt > 1:1034772/1‑69 (MQ=255) tctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCgtgt > 1:1936005/1‑69 (MQ=255) tctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCgtgt > 1:1130934/1‑69 (MQ=255) tctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCgtgt > 1:778581/1‑69 (MQ=255) tctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCgtgt > 1:3130352/1‑69 (MQ=255) tctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCgtgt > 1:1658533/1‑69 (MQ=255) tctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCgtgt > 1:1648658/1‑69 (MQ=255) tctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCgtgt > 1:1626836/1‑69 (MQ=255) tctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCgtgt > 1:1545198/1‑69 (MQ=255) tctTTGTCAGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCgtgt > 1:3032722/1‑69 (MQ=255) aGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTttgttg > 1:1527849/1‑69 (MQ=255) gCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTttgttg < 1:3170035/68‑1 (MQ=255) tCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCTCGCAGTTTGTTGGCGa < 1:1548328/69‑1 (MQ=255) tCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGa < 1:3389002/69‑1 (MQ=255) tCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGa < 1:724731/69‑1 (MQ=255) aTCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGAt < 1:2329885/67‑1 (MQ=255) aTCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGAt < 1:667563/67‑1 (MQ=255) aTCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGAt < 1:2985085/67‑1 (MQ=255) aTCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGAt < 1:2869183/67‑1 (MQ=255) aTCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGAt < 1:2217514/67‑1 (MQ=255) aTCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGAt < 1:2682520/67‑1 (MQ=255) aTCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATa < 1:2145746/68‑1 (MQ=255) aTCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATa < 1:410873/68‑1 (MQ=255) aTCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATa < 1:3007267/68‑1 (MQ=255) aTCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATa < 1:2717714/68‑1 (MQ=255) aTCGATTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGAt < 1:1570602/67‑1 (MQ=255) tCGCTTTGAAACATCGAGCAAAATGGCGCGATACAATTTACCGTGTCCGCGCAGTttg > 1:511755/1‑58 (MQ=255) tCGCTTTGAAACATCGAGCAAAATGGCGCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACt > 1:219174/1‑69 (MQ=255) tCGCTTTGAAACATCGAGCAAAATGGCGCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACt > 1:637469/1‑69 (MQ=255) tCGCTTTGAAACATCGAGCAAAATGGCGCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACt > 1:3405747/1‑69 (MQ=255) tCGCTTTGAAACATCGAGCAAAATGGCGCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACt > 1:2634487/1‑69 (MQ=255) tCGCTTTGAAACATCGAGCAAAATGGCGCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACt > 1:2611307/1‑69 (MQ=255) tCGCTTTGAAACATCGAGCAAAATGGCGCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACt > 1:2241975/1‑69 (MQ=255) tCGCTTTGAAACATCGAGCAAAATGGCGCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACt > 1:403724/1‑69 (MQ=255) tCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATAc < 1:2189579/68‑1 (MQ=255) tCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATAc < 1:2136886/68‑1 (MQ=255) tCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATAc < 1:2866756/68‑1 (MQ=255) tCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACt < 1:2394751/69‑1 (MQ=255) tCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACt < 1:2547929/69‑1 (MQ=255) gCTTTGAAACATCGAGCAAAATGGGCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTAt > 1:2472262/1‑69 (MQ=255) gCTTTGAAACATCGAGCAAAATGGCCCGATACAAt > 1:1809744/1‑35 (MQ=255) gCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCgtgt > 1:354954/1‑44 (MQ=255) gCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACt > 1:2526924/1‑67 (MQ=255) gCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACt > 1:2827182/1‑67 (MQ=255) gCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACt > 1:438900/1‑67 (MQ=255) gCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACt > 1:1666371/1‑67 (MQ=255) gCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACt > 1:539507/1‑67 (MQ=255) gCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACt > 1:1471584/1‑67 (MQ=255) gCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACt > 1:2341797/1‑67 (MQ=255) gCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACt > 1:3368706/1‑67 (MQ=255) ttGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGa > 1:3424408/1‑60 (MQ=255) ttGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGa > 1:1106263/1‑60 (MQ=255) ttGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGa > 1:3040338/1‑60 (MQ=255) ttGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGa > 1:2907090/1‑60 (MQ=255) ttGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGa > 1:2805360/1‑60 (MQ=255) ttGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGa > 1:808649/1‑60 (MQ=255) ttGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGa > 1:2268151/1‑60 (MQ=255) cATCGAGCAAAATGGCGCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATTCTATCGCCACCaa < 1:1295399/69‑1 (MQ=255) cATCGAGCAAAATGGCGCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCaa < 1:3295027/69‑1 (MQ=255) cATCGAGCAAAATGGCGCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCaa < 1:1764769/69‑1 (MQ=255) cATCGAGCAAAATGGCGCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCaa < 1:9577/69‑1 (MQ=255) cATCGAGCAAAATGGCGCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCaa < 1:594187/69‑1 (MQ=255) cATCGAGCAAAATGGCGCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCaa < 1:2426419/69‑1 (MQ=255) cATCGAGCAAAATGGCGCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCaa < 1:2471979/69‑1 (MQ=255) cATCGAGCAAAATGGCGCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCaa < 1:253044/69‑1 (MQ=255) cATCGAGCAAAATGGCGCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCaa < 1:346843/69‑1 (MQ=255) cATCGAGCAAAATGGCGCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCaa < 1:2809044/69‑1 (MQ=255) cATCGAGCAAAATGGCGCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCaa < 1:2812658/69‑1 (MQ=255) cATCGAGCAAAATGGCGCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCaa < 1:768063/69‑1 (MQ=255) cATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCaa < 1:2390216/69‑1 (MQ=255) cATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCaa < 1:747576/69‑1 (MQ=255) aTCGAGCAAAATGGCGCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCaa < 1:980074/68‑1 (MQ=255) gAGCAAAATGGCCCGATACAATTTAGCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATg > 1:1503687/1‑69 (MQ=255) gAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATg > 1:3318932/1‑69 (MQ=255) gAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATg > 1:3086162/1‑69 (MQ=255) gAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATg > 1:2712594/1‑69 (MQ=255) gAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATg > 1:838456/1‑69 (MQ=255) gAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATg > 1:2056371/1‑69 (MQ=255) gCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATtct > 1:1964505/1‑69 (MQ=255) | TTTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCT > minE/11020‑11153 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |