| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 785,203 | C→T | 41.2% | A14A (GCG→GCA) | dsbB ← | oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 785,203 | 0 | C | T | 41.2% | 40.4 / 69.0 | 97 | A14A (GCG→GCA) | dsbB | oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I |
| Reads supporting (aligned to +/- strand): ref base C (32/25); new base T (25/15); total (57/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.07e-01 | |||||||||||
TCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAG > minE/785139‑785271 | tCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACgcgc > 1:2932938/1‑69 (MQ=255)tCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACgcgc > 1:2069693/1‑69 (MQ=255)tCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACgcgc > 1:187393/1‑69 (MQ=255) cTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGcc > 1:1014482/1‑69 (MQ=255) cTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGcc > 1:2602714/1‑69 (MQ=255) cTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGcc > 1:2049371/1‑69 (MQ=255) cTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGcc > 1:1506028/1‑69 (MQ=255) cTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGcc > 1:663925/1‑69 (MQ=255) cTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGc > 1:354652/1‑68 (MQ=255) cTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGc > 1:1823071/1‑68 (MQ=255) cacaGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTTGTGaa < 1:2985039/69‑1 (MQ=255) cacaGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTTGTGaa < 1:1317850/69‑1 (MQ=255) cacaGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTTGTGaa < 1:2545692/69‑1 (MQ=255) cacaGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTTGTGaa < 1:3203644/69‑1 (MQ=255) cacaGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTTGTGaa < 1:1784179/69‑1 (MQ=255) cacaGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTTGTGaa < 1:206865/69‑1 (MQ=255) cacaGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTTGTGaa < 1:2319687/69‑1 (MQ=255) cacaGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTTGTGaa < 1:2221457/69‑1 (MQ=255) gCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCaa > 1:480189/1‑68 (MQ=255) gCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCaa > 1:664688/1‑68 (MQ=255) gTAAACGCCATCAACAGCCATGCGCTCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCAtt < 1:2500989/69‑1 (MQ=255) gTAAACGCCATCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCAtt < 1:3260902/69‑1 (MQ=255) gTAAACGCCATCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCAtt < 1:2195888/69‑1 (MQ=255) gTAAACGCCATCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCAtt < 1:2171464/69‑1 (MQ=255) gTAAACGCCATCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCAtt < 1:1124187/69‑1 (MQ=255) gTAAACGCCATCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCAtt < 1:3240237/69‑1 (MQ=255) gTAAACGCCATCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCAtt < 1:1129500/69‑1 (MQ=255) gTAAACGCCATCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCAtt < 1:870412/69‑1 (MQ=255) gTAAACGCCATCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCAtt < 1:2010537/69‑1 (MQ=255) gTAAACGCCATCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCAtt < 1:1934895/69‑1 (MQ=255) gTAAACGCCATCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCAtt < 1:2674292/69‑1 (MQ=255) gTAAACGCCATCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCAtt < 1:2815384/69‑1 (MQ=255) gTAAACGCCATCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCAtt < 1:584688/69‑1 (MQ=255) gTAAACGCCATCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCAtt < 1:1589182/69‑1 (MQ=255) gTAAACGCCATCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCAtt < 1:1858541/69‑1 (MQ=255) gCCATCAACAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATaa > 1:1170382/1‑58 (MQ=255) gCCATCAACAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTcc > 1:1075701/1‑66 (MQ=255) gCCATCAACAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCt > 1:1423677/1‑68 (MQ=255) gCCATCAACAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCt > 1:1942145/1‑68 (MQ=255) gCCATCAACAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCt > 1:2825615/1‑68 (MQ=255) gCCATCAACAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCt > 1:573662/1‑68 (MQ=255) gCCATCAACAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCt > 1:2669957/1‑68 (MQ=255) gCCATCAACAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCt > 1:2125068/1‑68 (MQ=255) gCCATCAACAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCt > 1:3310503/1‑68 (MQ=255) gCCATCAACAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCt > 1:157899/1‑68 (MQ=255) gCCATCAACAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCt > 1:60683/1‑68 (MQ=255) gCCATCAACAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCt > 1:3289234/1‑68 (MQ=255) gCCATCAACAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCt > 1:1473640/1‑68 (MQ=255) ccATCAACAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCt > 1:174591/1‑67 (MQ=255) ccATCAACAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGc < 1:499034/69‑1 (MQ=255) ccATCAACAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGc < 1:2618031/69‑1 (MQ=255) ccATCAACAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGc > 1:127142/1‑69 (MQ=255) ccATCAACAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGc > 1:2733088/1‑69 (MQ=255) ccATCAACAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGc > 1:563285/1‑69 (MQ=255) ccATCAACAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGc > 1:908930/1‑69 (MQ=255) ccATCAACAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGc > 1:353430/1‑69 (MQ=255) ccATCAACAGCCACGCGCCACGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTg > 1:1412521/1‑68 (MQ=255) tCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAAc > 1:3424036/1‑50 (MQ=255) tCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACa > 1:335133/1‑51 (MQ=255) tCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACa > 1:1037980/1‑51 (MQ=255) tCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACa > 1:920079/1‑51 (MQ=255) tCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACa > 1:701229/1‑51 (MQ=255) tCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACa > 1:3141438/1‑51 (MQ=255) tCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACa > 1:939650/1‑51 (MQ=255) tCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACa > 1:3131648/1‑51 (MQ=255) tCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACa > 1:3108847/1‑51 (MQ=255) tCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACa > 1:798496/1‑51 (MQ=255) tCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACa > 1:1892861/1‑51 (MQ=255) tCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACa > 1:1048998/1‑51 (MQ=255) tCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACa > 1:1166514/1‑51 (MQ=255) tCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACa > 1:1310607/1‑51 (MQ=255) tCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACa > 1:1376217/1‑51 (MQ=255) tCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACa > 1:1580040/1‑51 (MQ=255) tCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACa > 1:167295/1‑51 (MQ=255) tCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACa > 1:1741168/1‑51 (MQ=255) tCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACa > 1:1866098/1‑51 (MQ=255) tCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACa > 1:2991638/1‑51 (MQ=255) tCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACa > 1:2947545/1‑51 (MQ=255) tCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACa > 1:2780308/1‑51 (MQ=255) tCAACAGCCATGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACa > 1:2144768/1‑51 (MQ=255) tCAACAGCCATGCGCCCAGGCCTTGTGAACATTGGTTCAAAAATCGCAACa > 1:2983786/1‑51 (MQ=255) tCAACAGCCATGCGCACCGGCCTTGTGAACATTGGTTCAAAAATCGCAACa > 1:655672/1‑51 (MQ=255) aaCAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACAt < 1:1168374/50‑1 (MQ=255) aaCAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACAt < 1:2275407/50‑1 (MQ=255) cAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATg < 1:2042343/68‑1 (MQ=255) aGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCa < 1:1536804/69‑1 (MQ=255) aGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCa < 1:2202012/69‑1 (MQ=255) cACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATag < 1:2364639/69‑1 (MQ=255) cACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATag < 1:1916523/69‑1 (MQ=255) cACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATag < 1:133687/69‑1 (MQ=255) cACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATag < 1:2878626/69‑1 (MQ=255) cACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATag < 1:1272901/69‑1 (MQ=255) cACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATag < 1:192061/69‑1 (MQ=255) cACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATag < 1:2018692/69‑1 (MQ=255) cACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATag < 1:925110/69‑1 (MQ=255) cACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATag < 1:341445/69‑1 (MQ=255) aCGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATag < 1:328724/68‑1 (MQ=255) cgcgCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATagag < 1:1427709/69‑1 (MQ=255) | TCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAG > minE/785139‑785271 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |