| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 790,744 | T→C | 100% | N320N (AAT→AAC) | dadA → | D‑amino acid dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 790,744 | 0 | T | C | 76.4% | 51.2 / 10.5 | 42 | N320N (AAT→AAC) | dadA | D‑amino acid dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (2/8); new base C (32/0); total (34/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.81e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAATCGCCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAACGCTGGAGATGGTGGTTCGCGATCTCTATCCA > minE/790677‑790804 | aaaTCGCCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAAt < 1:1136045/68‑1 (MQ=255)aaaTCGCCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAAt < 1:2908368/68‑1 (MQ=255) ccATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGc > 1:801754/1‑69 (MQ=255) ccATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGc > 1:3098337/1‑69 (MQ=255) ccATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGc > 1:2918980/1‑69 (MQ=255) ccATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGc > 1:279888/1‑69 (MQ=255) ccATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGc > 1:2778845/1‑69 (MQ=255) ccATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGc > 1:2719050/1‑69 (MQ=255) ccATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGc > 1:2645235/1‑69 (MQ=255) ccATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGc > 1:3118505/1‑69 (MQ=255) ccATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGc > 1:3216193/1‑69 (MQ=255) ccATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGc > 1:2239231/1‑69 (MQ=255) ccATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGc > 1:2218896/1‑69 (MQ=255) ccATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGc > 1:3269896/1‑69 (MQ=255) ccATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGc > 1:3316963/1‑69 (MQ=255) ccATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGc > 1:1729656/1‑69 (MQ=255) ccATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGc > 1:167397/1‑69 (MQ=255) ccATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGc > 1:1655589/1‑69 (MQ=255) ccATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGc > 1:354175/1‑69 (MQ=255) ccATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGc > 1:1509719/1‑69 (MQ=255) ccATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGc > 1:738270/1‑69 (MQ=255) ccATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGc > 1:1257534/1‑69 (MQ=255) ccATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGc > 1:1177733/1‑69 (MQ=255) gTTTCGATAACCGCATTGGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGCTGTTGCAAc > 1:2327504/1‑69 (MQ=255) gTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGCTGTTGCAAc > 1:3223573/1‑69 (MQ=255) gTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGCTGTTGCAAc > 1:3322083/1‑69 (MQ=255) gTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGCTGTTGCAAc > 1:742356/1‑69 (MQ=255) gTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGCTGTTGCAAc > 1:817611/1‑69 (MQ=255) gTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGCTGTTGCAAc > 1:3058790/1‑69 (MQ=255) gTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGCTGTTGCAAc > 1:3037447/1‑69 (MQ=255) gTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGCTGTTGCAAc > 1:2449429/1‑69 (MQ=255) gTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGCTGTTGCAAc > 1:2238200/1‑69 (MQ=255) gTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGCTGTTGCAAc > 1:1284886/1‑69 (MQ=255) gTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAAATTGTTGGCTTTAACACCGAGCTGTTGCAAc > 1:1249744/1‑69 (MQ=255) gCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAACGCTGGAGATGGTGGTTcgc > 1:2052026/1‑69 (MQ=255) gCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAACGCTGGAGATGGTGGTTcgc > 1:1639341/1‑69 (MQ=255) tgttgGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAACGCTGGAGATGGTGGTTCGCGATCTCTa < 1:2968851/69‑1 (MQ=255) tgttgGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAACGCTGGAGATGGTGGTTCGCGATCTCTa < 1:1741087/69‑1 (MQ=255) tgttgGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAACGCTGGAGATGGTGGTTCGCGATCTCTa < 1:1670272/69‑1 (MQ=255) tgttgGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAACGCTGGAGATGGTGGTTCGCGATCTCTa < 1:3435551/69‑1 (MQ=255) tgttgGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAACGCTGGAGATGGTGGTTCGCGATCTCTa < 1:1099859/69‑1 (MQ=255) tgttgGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAACGCTGGAGATGGTGGTGCGCGATCTCTa < 1:2490906/69‑1 (MQ=255) gttgGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAACGCTGGAGATGGTGGTTCGCGATCTCTa < 1:1033937/68‑1 (MQ=255) ggTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAACGCTGGAGATGGTGGTTCGCGATCTCTATCCa < 1:3174548/69‑1 (MQ=255) | AAATCGCCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAACGCTGGAGATGGTGGTTCGCGATCTCTATCCA > minE/790677‑790804 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |