| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 791,603 | G→A | 59.5% | G171S (GGC→AGC) | dadX → | alanine racemase 2, PLP‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 791,603 | 0 | G | A | 59.5% | 25.2 / 48.3 | 69 | G171S (GGC→AGC) | dadX | alanine racemase 2, PLP‑binding |
| Reads supporting (aligned to +/- strand): ref base G (10/18); new base A (14/27); total (24/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01 | |||||||||||
GCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTC > minE/791537‑791667 | gCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGgc > 1:1741835/1‑69 (MQ=255)gCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGgc > 1:1049851/1‑69 (MQ=255)gCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGgc > 1:304899/1‑69 (MQ=255)gCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGgc > 1:2201137/1‑69 (MQ=255)gCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGgc > 1:3393809/1‑69 (MQ=255)gCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGgc > 1:2828597/1‑69 (MQ=255)gCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGgc > 1:1687584/1‑69 (MQ=255)gCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGgc > 1:1379540/1‑69 (MQ=255) gAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCTAAGCGGAACATCCTAATGGAATTTCCGgcgc < 1:2623161/69‑1 (MQ=255) gAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGgcgc < 1:1932496/69‑1 (MQ=255) gAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGgcgc < 1:463621/69‑1 (MQ=255) gAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGgcgc < 1:3088939/69‑1 (MQ=255) gAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGgcgc < 1:2853622/69‑1 (MQ=255) gAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGgcgc < 1:996127/69‑1 (MQ=255) gAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGgcgc < 1:3047584/69‑1 (MQ=255) gAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGcgcg < 1:1654602/68‑1 (MQ=255) gAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGt < 1:3370823/69‑1 (MQ=255) gAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGt < 1:2984395/69‑1 (MQ=255) gAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGt < 1:1930245/69‑1 (MQ=255) gAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGt < 1:1288629/69‑1 (MQ=255) gAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGt < 1:867354/69‑1 (MQ=255) gAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGt < 1:785741/69‑1 (MQ=255) gAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCAGCGCGATGGCGCGt < 1:1917042/69‑1 (MQ=255) gAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCAGCGCGATGGCGCGt < 1:1038038/69‑1 (MQ=255) gAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCAGCGCGATGGCGCGt < 1:987056/69‑1 (MQ=255) gAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCAGCGCGATGGCGCGt < 1:1620215/69‑1 (MQ=255) gAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCAGCGCGATGGCGCGt < 1:2243017/69‑1 (MQ=255) gAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCAGCGCGATGGCGCGt < 1:1734894/69‑1 (MQ=255) gAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCAGCGCGATGGCGCGt < 1:47255/69‑1 (MQ=255) gAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCAGCGCGATGGCGCGt < 1:486227/69‑1 (MQ=255) gAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCAGCGCGATGGCGCGt < 1:2044247/69‑1 (MQ=255) gAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCAGCGCGATGGCGCGt < 1:1986982/69‑1 (MQ=255) gAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCAGCGCGATGGCGCGt < 1:530790/69‑1 (MQ=255) gAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCAGCGCGATGGCGCGt < 1:702862/69‑1 (MQ=255) tCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAggcgg > 1:2802052/1‑67 (MQ=255) gAAGCGTAACATCCTGATGGATTTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGAAGGGCTGGa < 1:1209027/68‑1 (MQ=255) gAAGCGGAACATCCTGATGGAATTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGAAGGGCTGGa < 1:757673/68‑1 (MQ=255) gAAGCGGAACATCCTGATGGAATTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGAAGGGCTGGa < 1:360336/68‑1 (MQ=255) gAAGCGGAACATCCTGATGGAATTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGAAGGGCTGGa < 1:551920/68‑1 (MQ=255) gAAGCGGAACATCCTGATGGAATTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGAAGGGCTGGa < 1:994911/68‑1 (MQ=255) gAAGCGGAACATCCTGATGGAATTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGAAGGGCTGGa < 1:998266/68‑1 (MQ=255) gAAGCGGAACATCCTGATGGAATTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGAAGGGCTGGa < 1:1001629/68‑1 (MQ=255) gAAGCGGAACATCCTGATGGAATTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGAAGGGCTGGa < 1:555895/68‑1 (MQ=255) gAAGCGGAACATCCTGATGGAATTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGAAGGGCTGGa < 1:1113885/68‑1 (MQ=255) gAAGCGGAACATCCTGATGGAATTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGAAGGGCTGGa < 1:1366444/68‑1 (MQ=255) gAAGCGGAACATCCTGATGGAATTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGAAGGGCTGGa < 1:2499255/68‑1 (MQ=255) gAAGCGGAACATCCTGATGGAATTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGAAGGGCTGGa < 1:1727833/68‑1 (MQ=255) gAAGCGGAACATCCTGATGGAATTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGAAGGGCTGGa < 1:2120013/68‑1 (MQ=255) gAAGCGGAACATCCTGATGGAATTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGAAGGGCTGGa < 1:193352/68‑1 (MQ=255) gAAGCGGAACATCCTGATGGAATTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGAAGGGCTGGa < 1:3007311/68‑1 (MQ=255) ggAACATCCTGATGGAATTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGaag > 1:1984765/1‑54 (MQ=39) ggAACATCCTGATGGAATTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGaag > 1:116046/1‑54 (MQ=39) ggAACATCCTGATGGAATTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGaag > 1:1299520/1‑54 (MQ=39) ggAACATCCTGATGGAATTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGaag > 1:1359274/1‑54 (MQ=39) ggAACATCCTGATGGAATTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGaag > 1:1723489/1‑54 (MQ=39) ggAACATCCTGATGGAATTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGaag > 1:1831860/1‑54 (MQ=39) ggAACATCCTGATGGAATTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGaag > 1:638889/1‑54 (MQ=39) ggAACATCCTGATGGAATTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGaag > 1:514389/1‑54 (MQ=39) ggAACATCCTGATGGAATTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGaag > 1:2194098/1‑54 (MQ=39) ggAACATCCTGATGGAATTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGaag > 1:2479974/1‑54 (MQ=39) ggAACATCCTGATGGAATTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGaag > 1:2606594/1‑54 (MQ=39) ggAACATCCTGATGGAATTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGaag > 1:3354382/1‑54 (MQ=39) ggAACATCCTGATGGAATTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGaag > 1:3109484/1‑54 (MQ=39) ggAACATCCAGATGGAATTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGaag > 1:1916194/1‑54 (MQ=38) tCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGcgttc > 1:2321493/1‑69 (MQ=255) ttCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTc < 1:3362768/69‑1 (MQ=255) ttCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTc < 1:878266/69‑1 (MQ=255) ttCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTc < 1:3125717/69‑1 (MQ=255) ttCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTc < 1:2634175/69‑1 (MQ=255) | GCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTC > minE/791537‑791667 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |