| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 78,700 | T→C | 28.0% | I265I (ATT→ATC) | murE → | UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate:meso‑ diaminopimelate ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 78,700 | 0 | T | C | 28.0% | 90.5 / 37.4 | 89 | I265I (ATT→ATC) | murE | UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate:meso‑ diaminopimelate ligase |
| Reads supporting (aligned to +/- strand): ref base T (39/25); new base C (10/15); total (49/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.80e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.39e-01 | |||||||||||
TGGGCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGCGACGA > minE/78639‑78767 | tGGGCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGa < 1:2447306/69‑1 (MQ=255)tGGGCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGa < 1:21288/69‑1 (MQ=255)tGGGCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGa < 1:1656341/69‑1 (MQ=255) ggCCGCCGCTGGCTGTCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAAc < 1:2549785/69‑1 (MQ=255) ggCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAAc < 1:2138665/69‑1 (MQ=255) ggCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAAc < 1:1571844/69‑1 (MQ=255) cgccgcTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTTATCCGAACTGt < 1:46390/69‑1 (MQ=255) cgccgcTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAACTGt < 1:1220632/69‑1 (MQ=255) cgccgcTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAACTGt < 1:233159/69‑1 (MQ=255) cgccgcTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAACTGt < 1:2049469/69‑1 (MQ=255) cgccgcTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAACTGt < 1:1763523/69‑1 (MQ=255) cgccgcTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAACTGt < 1:1297413/69‑1 (MQ=255) cTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGaaa > 1:1223592/1‑69 (MQ=255) cTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGaaa > 1:128070/1‑69 (MQ=255) cTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGaaa > 1:2614575/1‑69 (MQ=255) cTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGaa > 1:353790/1‑68 (MQ=255) cTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGaa > 1:3034559/1‑68 (MQ=255) gCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGc < 1:2328883/69‑1 (MQ=255) gCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGc < 1:726957/69‑1 (MQ=255) aCGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCg < 1:494715/69‑1 (MQ=255) aCGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCg < 1:3140414/69‑1 (MQ=255) aCGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCg < 1:2652709/69‑1 (MQ=255) ttGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGa < 1:3314140/69‑1 (MQ=255) ttGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGa < 1:2134560/69‑1 (MQ=255) ttGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGa < 1:2527065/69‑1 (MQ=255) ttGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGa < 1:648582/69‑1 (MQ=255) ttGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGa < 1:485643/69‑1 (MQ=255) ttGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGa < 1:2955001/69‑1 (MQ=255) tGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGa < 1:2453648/68‑1 (MQ=255) tGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGa < 1:3182933/68‑1 (MQ=255) tttCAATGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACt < 1:2989467/64‑1 (MQ=255) tATCAATGGAAGATCATATCAATCCGAACTGTCACGGACGCTTGTTGAAAGCGACCGACGTGAACt < 1:3220212/66‑1 (MQ=255) tATCAATGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACt < 1:1128825/66‑1 (MQ=255) tATCAATGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACt < 1:3127287/66‑1 (MQ=255) tATCAATGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACt < 1:2656285/66‑1 (MQ=255) tATCAATGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACt < 1:2335882/66‑1 (MQ=255) tATCAATGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACt < 1:2532731/66‑1 (MQ=255) tATCAATGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACt < 1:1652857/66‑1 (MQ=255) tATCAATGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACt < 1:202632/66‑1 (MQ=255) tATCAATGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACt < 1:1415905/66‑1 (MQ=255) tATCAATGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACt < 1:1449135/66‑1 (MQ=255) tATCAATGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACt < 1:1512196/66‑1 (MQ=255) tATCAATGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACt < 1:2030250/66‑1 (MQ=255) tATCAATGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACt < 1:1551625/66‑1 (MQ=255) aTCAATGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACt < 1:1602527/65‑1 (MQ=255) tCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCAc > 1:3429244/1‑69 (MQ=255) aaTGGATGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACTATCAc > 1:643/1‑67 (MQ=255) aaTGGAAGATCATATTAATCCGAACTGTCACGGACGc > 1:1495137/1‑37 (MQ=255) aaTGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCAc > 1:1832636/1‑67 (MQ=255) aaTGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCAc > 1:982987/1‑67 (MQ=255) aaTGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCAc > 1:1536722/1‑67 (MQ=255) aaTGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCAc > 1:140527/1‑67 (MQ=255) aaTGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCAc > 1:648595/1‑67 (MQ=255) aaTGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCAc > 1:870183/1‑67 (MQ=255) aaTGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCAc > 1:2307748/1‑67 (MQ=255) aaTGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCAc > 1:2660915/1‑67 (MQ=255) aaTGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCAc > 1:2078403/1‑67 (MQ=255) aaTGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCAc > 1:2269069/1‑67 (MQ=255) aaTGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCAc > 1:2035680/1‑67 (MQ=255) aaTGGAAGATCATATTAATCCGAACTGTCA‑GGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGAc > 1:1972871/1‑69 (MQ=255) aaTGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACTATCAc > 1:2673934/1‑67 (MQ=255) aaTGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACTATCAc > 1:1353118/1‑67 (MQ=255) aaTGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACTATCAc > 1:800661/1‑67 (MQ=255) aaTGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACTATCAc > 1:360024/1‑67 (MQ=255) aaTGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACTATCAc > 1:832200/1‑67 (MQ=255) aaTGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACTATCAc > 1:854083/1‑67 (MQ=255) aaTGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACTATCAc > 1:1978736/1‑67 (MQ=255) aaTGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACTATCAc > 1:17513/1‑67 (MQ=255) aaTGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACAGACGTGAACTATCAc > 1:259788/1‑67 (MQ=255) gAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTAt > 1:1485784/1‑60 (MQ=255) gAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTAt > 1:2337353/1‑60 (MQ=255) gAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTAt > 1:687971/1‑60 (MQ=255) gAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTAt > 1:524526/1‑60 (MQ=255) gAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTAt > 1:3274918/1‑60 (MQ=255) gAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTAt > 1:2916029/1‑60 (MQ=255) gAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTAt > 1:3429936/1‑60 (MQ=255) gAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTAt > 1:2566439/1‑60 (MQ=255) tCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGc > 1:2485892/1‑69 (MQ=255) tCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGc > 1:583417/1‑69 (MQ=255) cATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCTAAGTGAACTATCACGACAGCGGTGcg > 1:442973/1‑69 (MQ=255) cATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGcg > 1:919314/1‑69 (MQ=255) cATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGcg > 1:2705210/1‑69 (MQ=255) cATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGcg > 1:710699/1‑69 (MQ=255) cATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGcg > 1:2174981/1‑69 (MQ=255) cATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGcg > 1:2121828/1‑69 (MQ=255) cATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGcg > 1:3325574/1‑69 (MQ=255) cATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGcg > 1:1621233/1‑69 (MQ=255) cATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGcg > 1:2714882/1‑69 (MQ=255) cATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGc > 1:518309/1‑68 (MQ=255) ttAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGcgacga > 1:2497210/1‑69 (MQ=255) ttAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGcgacga > 1:2542290/1‑69 (MQ=255) ttAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGcgacg > 1:2333493/1‑68 (MQ=255) | TGGGCCGCCGCTGGCTGGCAAAACTGCCGGACGCGGTTGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGCGACGA > minE/78639‑78767 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |