Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 796,571 | A→G | 36.4% | S57S (AGT→AGC) | ycgR ← | protein involved in flagellar function |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 796,571 | 0 | A | G | 36.4% | 49.1 / 51.6 | 85 | S57S (AGT→AGC) | ycgR | protein involved in flagellar function |
Reads supporting (aligned to +/- strand): ref base A (33/21); new base G (13/18); total (46/39) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.15e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01 |
ACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTGATCAGCTG > minE/796505‑796630 | aCCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACt > 1:265118/1‑69 (MQ=255) aCCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACt > 1:3414654/1‑69 (MQ=255) ttCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATc < 1:41788/69‑1 (MQ=255) ttCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATc < 1:1591505/69‑1 (MQ=255) ttCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATc < 1:737545/69‑1 (MQ=255) ttCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATc < 1:2973364/69‑1 (MQ=255) ttCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATc < 1:2331606/69‑1 (MQ=255) tCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATc < 1:2559930/68‑1 (MQ=255) aatggtaatgTGCTTTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAg < 1:611662/69‑1 (MQ=255) aatggtaatgTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAg < 1:892774/69‑1 (MQ=255) aatggtaatgTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAg < 1:2329259/69‑1 (MQ=255) aatggtaatgTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAg < 1:2705993/69‑1 (MQ=255) aatggtaatgTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAg < 1:2587772/69‑1 (MQ=255) aatggtaatgTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAg < 1:1693466/69‑1 (MQ=255) gtgctgtgcCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCgg < 1:2762299/69‑1 (MQ=255) tgctgtgcCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCggg > 1:2441536/1‑69 (MQ=255) tgctgtgcCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCggg > 1:2433638/1‑69 (MQ=255) tgctgtgcCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCggg > 1:2698652/1‑69 (MQ=255) tgctgtgcCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCggg > 1:2607288/1‑69 (MQ=255) tgctgtgcCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCggg > 1:1243786/1‑69 (MQ=255) tgctgtgcCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCggg > 1:539980/1‑69 (MQ=255) tgctgtgcCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCggg > 1:460908/1‑69 (MQ=255) tgctgtgcCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCggg > 1:1002754/1‑69 (MQ=255) tgctgtgcCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCggg > 1:1454315/1‑69 (MQ=255) tgctgcgcCTTTAGCACGGCGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATc > 1:2358138/1‑65 (MQ=255) tgctgcgcCTTTAGCACGGCGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATCCgga > 1:778469/1‑68 (MQ=255) tgctgcgcCTTTAGCACGGCGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATCCgga > 1:1250888/1‑68 (MQ=255) tgctgcgcCTTTAGCACGGCGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATCCgga > 1:2556711/1‑68 (MQ=255) tgctgcgcCTTTAGCACGGCGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATCCgga > 1:1280398/1‑68 (MQ=255) tgctgcgcCTTTAGCACGGCGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATCCgga > 1:919085/1‑68 (MQ=255) tgctgcgcCTTTAGCACGGCGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATCCgga > 1:1102682/1‑68 (MQ=255) tgctgcgcCTTTAGCACGGCGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATCCgga > 1:782360/1‑68 (MQ=255) tgctgcgcCTTTAGCACGGCGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATCCgga > 1:2279073/1‑68 (MQ=255) tgctgcgcCTTTAGCACGGCGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATCCgga > 1:3400566/1‑68 (MQ=255) tgctgcgcCTTTAGCACGGCGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATCCgga > 1:670775/1‑68 (MQ=255) tgctgcgcCTTTAGCACGGCGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATCCgga > 1:371401/1‑68 (MQ=255) tgctgcgcCTTTAGCACGGCGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATCCgga > 1:44054/1‑68 (MQ=255) tgcCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTAt > 1:1007158/1‑69 (MQ=255) tgcCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTAt > 1:911180/1‑69 (MQ=255) tgcCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTAt > 1:787873/1‑69 (MQ=255) tgcCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTAt > 1:3238713/1‑69 (MQ=255) tgcCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTAt > 1:3348212/1‑69 (MQ=255) tgcCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTAt > 1:2204236/1‑69 (MQ=255) tgcCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTAt > 1:2576353/1‑69 (MQ=255) tgcCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTAt > 1:1731973/1‑69 (MQ=255) ccTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCgggg > 1:2281006/1‑63 (MQ=255) ccTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGtt > 1:2085166/1‑65 (MQ=255) ccTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGtt > 1:3368666/1‑65 (MQ=255) ccTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGtt > 1:1514593/1‑65 (MQ=255) ccTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGtt > 1:2761259/1‑65 (MQ=255) ccTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGtt > 1:2605214/1‑65 (MQ=255) ccTTTAGCAAGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGtt > 1:831047/1‑54 (MQ=255) cGATGTTGTCTTCGGCCTGGCTGCCGACATCCAGCACCAGTTTATCCGGAGTTATTGCCAGTATTTTGc < 1:3015201/69‑1 (MQ=255) cGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATCCGGAGTTATTGCCAGTCTTTTGc < 1:438993/69‑1 (MQ=255) cGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATCCGGAGTTATTGCCAGTATTTTGc < 1:2006174/69‑1 (MQ=255) cGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATCCGGAGTTATTGCCAGTATTTTGc < 1:631069/69‑1 (MQ=255) cGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATCCGGAGTTATTGCCAGTATTTTGc < 1:38060/69‑1 (MQ=255) cGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATCCGGAGTTATTGCCAGTATTTTGc < 1:3426847/69‑1 (MQ=255) cGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATCCGGAGTTATTGCCAGTATTTTGc < 1:1664763/69‑1 (MQ=255) cGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATCCGGAGTTATTGCCAGTATTTTGc < 1:2220600/69‑1 (MQ=255) cGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATCCGGAGTTATTGCCAGTATTTTGc < 1:15145/69‑1 (MQ=255) cGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATCCGGAGTTATTGCCAGTATTTTGc < 1:1479420/69‑1 (MQ=255) cGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATCCGGAGTTATTGCCAGTATTTTGc < 1:2260856/69‑1 (MQ=255) cGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATCCGGAGTTATTGCCAGTATTTTGc < 1:3211947/69‑1 (MQ=255) cGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATCCGGAGTTATTGCCAGTATTTTGc < 1:2974818/69‑1 (MQ=255) cGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATCCGGAGTTATTGCCAGTATTTTGc < 1:2818713/69‑1 (MQ=255) cGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATCCGGAGTTATTGCCAGTATTTTGc < 1:1346802/69‑1 (MQ=255) cGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATCCGGAGTTATTGCCAGTATTTTGc < 1:271670/69‑1 (MQ=255) cGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATCCGGAGTTATTGCCAGTATTTTGc < 1:2598855/69‑1 (MQ=255) cGATGTTGTCTTCGGCCTGGCTGCCGAAATCCAGCACCAGTTTATCCGGAGTTATTGCCAGTATTTGGc < 1:1464929/69‑1 (MQ=255) aTGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTg < 1:766765/69‑1 (MQ=255) aTGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTg < 1:1183170/69‑1 (MQ=255) aTGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTg < 1:2099421/69‑1 (MQ=255) aTGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTg < 1:329187/69‑1 (MQ=255) gttgtCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCt > 1:644168/1‑66 (MQ=255) gttgtCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTg > 1:701318/1‑67 (MQ=255) gttgtCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTg > 1:1536530/1‑67 (MQ=255) gttgtCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTg > 1:1975355/1‑67 (MQ=255) gttgtCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTg > 1:2226759/1‑67 (MQ=255) gttgtCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTg > 1:3122782/1‑67 (MQ=255) gttgtCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTg > 1:3053846/1‑67 (MQ=255) ttCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTGATCAGCTg < 1:171198/69‑1 (MQ=255) ttCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTGATCAGCTg < 1:2513226/69‑1 (MQ=255) ttCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTGATCAGCTg < 1:2625729/69‑1 (MQ=255) ttCGGCTTGACTGCCGAAATCCAGCACCAGTCTATCCGGGGTTATTGCCAGTAATTTGCTGATCAGCTg < 1:541710/69‑1 (MQ=255) | ACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTGATCAGCTG > minE/796505‑796630 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |