| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 800,303 | T→C | 65.5% | A342A (GCA→GCG) | dhaH ← | fused predicted dihydroxyacetone‑specific PTS enzyme HPr component and EI component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 800,303 | 0 | T | C | 65.5% | 35.2 / 27.5 | 52 | A342A (GCA→GCG) | dhaH | fused predicted dihydroxyacetone‑specific PTS enzyme HPr component and EI component |
| Reads supporting (aligned to +/- strand): ref base T (10/8); new base C (20/14); total (30/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTG > minE/800242‑800370 | catcatCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGc > 1:3264195/1‑69 (MQ=255)catcatCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGc > 1:125158/1‑69 (MQ=255)catcatCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGc > 1:2688270/1‑69 (MQ=255)catcatCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGc > 1:1995181/1‑69 (MQ=255)catcatCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGc > 1:799847/1‑69 (MQ=255)catcatCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGc > 1:2109934/1‑69 (MQ=255)catcatCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGc > 1:1729271/1‑69 (MQ=255)catcatCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGc > 1:1748912/1‑69 (MQ=255) ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGtt < 1:3308499/69‑1 (MQ=255) ccAGTTGCTGGTATTGATGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGtt < 1:2600959/69‑1 (MQ=255) ggTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGa > 1:3068163/1‑68 (MQ=255) ggTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGa > 1:249151/1‑68 (MQ=255) ggTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGa > 1:2422024/1‑68 (MQ=255) ggTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATACCGCCGTGCAATGTTCATGCTGa > 1:2211207/1‑68 (MQ=255) ttGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAg < 1:3053374/69‑1 (MQ=255) ttGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAg < 1:2050571/69‑1 (MQ=255) gCTGGCTAAGTTCTTTAGGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGAAGGAGt < 1:2255733/68‑1 (MQ=255) gCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGAAGGAGt < 1:2207841/68‑1 (MQ=255) gCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGAAGGAGt < 1:3168753/68‑1 (MQ=255) gCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGAAGGAGt < 1:3098198/68‑1 (MQ=255) gCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGAAGGAGt < 1:1835241/68‑1 (MQ=255) gCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGAAGGAGt < 1:3257582/68‑1 (MQ=255) gCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGAAGGAGt < 1:1901913/68‑1 (MQ=255) gCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGAAGGAGt < 1:502002/68‑1 (MQ=255) gCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGAAGGAGt < 1:1693485/68‑1 (MQ=255) gCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGAAGGAGt < 1:69850/68‑1 (MQ=255) gCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGAAGGAGt < 1:753576/68‑1 (MQ=255) gCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGAAGGAGt < 1:1418188/68‑1 (MQ=255) gCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGAAGGAGt < 1:917321/68‑1 (MQ=255) gCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGAAGGAGt < 1:1193100/68‑1 (MQ=255) tAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGc > 1:1384118/1‑53 (MQ=255) tAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGAAgg > 1:946438/1‑59 (MQ=255) tAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGAAgg > 1:566980/1‑59 (MQ=255) tAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGAAgg > 1:588039/1‑59 (MQ=255) tAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGAAgg > 1:1017667/1‑59 (MQ=255) tAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGAAgg > 1:2577683/1‑59 (MQ=255) tAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGAAgg > 1:1408886/1‑59 (MQ=255) tAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGAAgg > 1:1412945/1‑59 (MQ=255) tAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGAAgg > 1:1464373/1‑59 (MQ=255) tAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGAAgg > 1:151089/1‑59 (MQ=255) tAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGAAgg > 1:1697669/1‑59 (MQ=255) tAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGAAgg > 1:1761904/1‑59 (MQ=255) tAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGAAgg > 1:2017035/1‑59 (MQ=255) tAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGAAgg > 1:2023911/1‑59 (MQ=255) tAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGAAgg > 1:2212591/1‑59 (MQ=255) tAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCCGCCGTGCAATGTTCATGCTGAAgg > 1:2793489/1‑59 (MQ=255) aGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTgcc > 1:3227443/1‑69 (MQ=255) aGTTCTTTAAGAACTTGCTGCAAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTgcc > 1:451122/1‑69 (MQ=255) aGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGatca < 1:3277839/68‑1 (MQ=255) aGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGatca < 1:2539358/68‑1 (MQ=255) cTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAgtg < 1:1721774/69‑1 (MQ=255) cTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAgtg < 1:1489694/69‑1 (MQ=255) tGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAgtg < 1:1778380/68‑1 (MQ=255) | CATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTG > minE/800242‑800370 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |