| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 800,666 | C→T | 36.9% | G221G (GGG→GGA) | dhaH ← | fused predicted dihydroxyacetone‑specific PTS enzyme HPr component and EI component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 800,666 | 0 | C | T | 36.9% | 51.1 / 47.9 | 81 | G221G (GGG→GGA) | dhaH | fused predicted dihydroxyacetone‑specific PTS enzyme HPr component and EI component |
| Reads supporting (aligned to +/- strand): ref base C (26/25); new base T (14/16); total (40/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01 | |||||||||||
TCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATA > minE/800598‑800729 | tcctccGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGc < 1:1488058/69‑1 (MQ=255) ttCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGc < 1:2748415/69‑1 (MQ=255) aCCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCaa < 1:1180429/68‑1 (MQ=255) ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGcc < 1:2863186/68‑2 (MQ=255) ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:20176/68‑1 (MQ=255) ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:2030303/68‑1 (MQ=255) ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:2049702/68‑1 (MQ=255) ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:172245/68‑1 (MQ=255) ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:2377722/68‑1 (MQ=255) ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:2443062/68‑1 (MQ=255) ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:2592635/68‑1 (MQ=255) ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:2646498/68‑1 (MQ=255) ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:1345051/68‑1 (MQ=255) ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:1337067/68‑1 (MQ=255) ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:1331440/68‑1 (MQ=255) ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:2950304/68‑1 (MQ=255) ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:3303494/68‑1 (MQ=255) ttCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:690354/68‑1 (MQ=255) tCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGt < 1:1302530/68‑1 (MQ=255) gccagcTGACGGAAAGCGATCAGTGCTTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTAt < 1:3131887/67‑1 (MQ=255) gccagcTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGt < 1:657805/65‑1 (MQ=255) gccagcTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGt < 1:216088/65‑1 (MQ=255) gccagcTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGt < 1:796020/65‑1 (MQ=255) gccagcTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGt < 1:992531/65‑1 (MQ=255) gccagcTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGt < 1:1603421/65‑1 (MQ=255) ccagcTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGt < 1:1872696/64‑1 (MQ=255) ccagcTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGt < 1:1684866/69‑1 (MQ=255) ccagcTGACGGAAAGCGATCAGTGCATCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGt < 1:2906203/69‑1 (MQ=255) aGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg < 1:3205199/69‑1 (MQ=255) aGCGATCAGTGCATCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg < 1:951448/69‑1 (MQ=255) gCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg < 1:2562522/68‑1 (MQ=255) aTCAGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCg < 1:3041843/46‑1 (MQ=255) cAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCg < 1:242628/57‑1 (MQ=255) aGTGCCTCTTTAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTgtagt > 1:3004697/1‑66 (MQ=255) aGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTgtagt > 1:2732034/1‑66 (MQ=255) aGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTgtagt > 1:27070/1‑66 (MQ=255) aGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTgtagt > 1:2865226/1‑66 (MQ=255) aGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTgtagt > 1:1261778/1‑66 (MQ=255) aGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTgtagt > 1:2967217/1‑66 (MQ=255) aGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTgtagt > 1:2994995/1‑66 (MQ=255) aGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTgtagt > 1:3087548/1‑66 (MQ=255) aGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTgtagt > 1:2202601/1‑66 (MQ=255) aGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTgtagt > 1:3418887/1‑66 (MQ=255) gCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCa < 1:2988502/69‑1 (MQ=255) gCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCa < 1:2266185/69‑1 (MQ=255) gCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCa < 1:2882896/69‑1 (MQ=255) ccTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCaa > 1:3156922/1‑69 (MQ=255) ccTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCaa > 1:3387166/1‑69 (MQ=255) ccTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCaa > 1:1494252/1‑69 (MQ=255) ccTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCaa > 1:2044782/1‑69 (MQ=255) ccTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCaa > 1:2258429/1‑69 (MQ=255) cAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTgg > 1:2721440/1‑68 (MQ=255) cAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTgg > 1:2724056/1‑68 (MQ=255) cAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTgg > 1:2626703/1‑68 (MQ=255) cAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTgg > 1:2097217/1‑68 (MQ=255) cAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTgg > 1:1134057/1‑68 (MQ=255) cAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTgg > 1:1123125/1‑68 (MQ=255) cAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTgg > 1:2369143/1‑68 (MQ=255) cAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTg > 1:2363585/1‑67 (MQ=255) cAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTg > 1:3423869/1‑67 (MQ=255) cAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTg > 1:2076108/1‑67 (MQ=255) cAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTg > 1:1393026/1‑67 (MQ=255) gCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGtt < 1:2131304/68‑1 (MQ=255) gCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGtt < 1:3343861/68‑1 (MQ=255) gCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGt < 1:115688/67‑1 (MQ=255) gCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGt < 1:1778835/67‑1 (MQ=255) gCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGt < 1:2643515/67‑1 (MQ=255) tCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg > 1:1057596/1‑47 (MQ=255) tCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg > 1:762522/1‑47 (MQ=255) tCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg > 1:1107719/1‑47 (MQ=255) tCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg > 1:1091136/1‑47 (MQ=255) tCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg > 1:973072/1‑47 (MQ=255) tCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg > 1:621116/1‑47 (MQ=255) tCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg > 1:392418/1‑47 (MQ=255) tCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg > 1:3247342/1‑47 (MQ=255) tCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg > 1:1766277/1‑47 (MQ=255) tCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg > 1:2387674/1‑47 (MQ=255) tCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg > 1:2424795/1‑47 (MQ=255) tCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg > 1:2433031/1‑47 (MQ=255) tCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg > 1:1567584/1‑47 (MQ=255) tCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg > 1:1559646/1‑47 (MQ=255) tCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATa < 1:428910/69‑1 (MQ=255) | TCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATA > minE/800598‑800729 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |