| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 804,017 | G→A | 51.6% | R246R (CGG→CGA) | dhaR → | predicted DNA‑binding transcriptional regulator, dihydroxyacetone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 804,017 | 0 | G | A | 51.6% | ‑3.7 / 53.2 | 60 | R246R (CGG→CGA) | dhaR | predicted DNA‑binding transcriptional regulator, dihydroxyacetone |
| Reads supporting (aligned to +/- strand): ref base G (14/15); new base A (17/14); total (31/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
TTGCAATTTATTAATGCCCAGGCGGCGCGGGTCTTGCGCCTTGACGCGACGGCAAGTCAGGGACGGGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCG > minE/803952‑804080 | ttGCAATTTATTAATGCCCAGGCGGCGCGGGTCTTGCGCCTTGACGCGACGGCAAGTCAGTGACGGGCa > 1:2919863/1‑69 (MQ=255)ttGCAATTTATTAATGCCCAGGCGGCGCGGGTCTTGCGCCTTGACGCGACGGCAAGTCAGTGACGGGCa > 1:2923751/1‑69 (MQ=255)ttGCAATTTATTAATGCCCAGGCGGCGCGGGTCTTGCGCCTTGACGCGACGGCAAGTCAGGGACGGGCa > 1:237378/1‑69 (MQ=255)ttGCAATTTATTAATGCCCAGGCGGCGCGGGTCTTGCGCCTTGACGCGACGGCAAGTCAGGGACGGGCa > 1:3408486/1‑69 (MQ=255) ttattaATGCCCAGGCGGCGCGGGTCTTGCGCCTTGACGCGACGGCAAGTCAGGGACGGGCAATCa < 1:232387/66‑1 (MQ=255) gCCCAGGCGGCGCGGGTCTTGCGCCTTGACGCGACGGCAAGTCAGGGACGGGCAATCACTGAACTCtt < 1:77267/68‑1 (MQ=255) gCCCAGGCGGCGCGGGTCTTGCGCCTTGACGCGACGGCAAGTCAGGGACGGGCAATCACTGAACTCtt < 1:541111/68‑1 (MQ=255) gCCCAGGCGGCGCGGGTCTTGCGCCTTGACGCGACGGCAAGTCAGGGACGGGCAATCACTGAACTCtt < 1:45875/68‑1 (MQ=255) gCCCAGGCGGCGCGGGTCTTGCGCCTTGACGCGACGGCAAGTCAGGGACGGGCAATCACTGAACTCtt < 1:276127/68‑1 (MQ=255) gCCCAGGCGGCGCGGGTCTTGCGCCTTGACGCGACGGCAAGTCAGGGACGGGCAATCACTGAACTCtt < 1:1625923/68‑1 (MQ=255) ggcgCGGGACTTGCGCCTTGACGCGACGGCAAGTCAGGGACGGGCAATCa < 1:681833/50‑1 (MQ=255) gcgGGTCTTGCGCCTTGACGCGACGGCAAGTCAGGGACGGGCAATCACTGAACTCTTAACGTTACccgc > 1:244846/1‑69 (MQ=255) cTTGCGCCTTGACGCGACGGCAAGTCAGGGACGGGCAATCACTGAACTCTTAACGTTACCCGCCGTAtt < 1:2497559/69‑1 (MQ=255) ttGCGCCTTGACGCGACGGCAAGTCAGGGACGGGCAATCACTGAACTCTTAACGTTACCCGCCGTAtt < 1:1135275/68‑1 (MQ=255) gcgcCTTGACGCGACGGCAAGTCAGGGACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGc < 1:3427097/68‑1 (MQ=255) gcgcCTTGACGCGACGGCAAGTCAGGGACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGc < 1:1334529/68‑1 (MQ=255) gcgttGACGCGACGGCAAGTCAGGGTCGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGc < 1:1752054/63‑1 (MQ=255) gcCTTGACGCGACGGCAAGTCATGGACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGc < 1:2307987/66‑1 (MQ=255) gcCTTGACGCGACGGCAAGTCAGGGACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGc < 1:301860/66‑1 (MQ=255) gcCTTGACGCGACGGCAAGTCAGGGACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGc < 1:1655969/66‑1 (MQ=255) gcCTTGACGCGACGGCAAGTCAGGGACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGc < 1:1685450/66‑1 (MQ=255) gcCTTGACGCGACGGCAAGTCAGGGACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGc < 1:269073/66‑1 (MQ=255) gcCTTGACGCGACGGCAAGTCAGGGACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGc < 1:2677717/66‑1 (MQ=255) gcCTTGACGCGACGGCAAGTCAGGGACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGc < 1:3427311/66‑1 (MQ=255) gcCTTGACGCGACGGCAAGTCAGGGACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGc < 1:1222761/66‑1 (MQ=255) ccTTGACGCGACGGCAAGTCAGGGACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGc < 1:1918640/65‑1 (MQ=255) ccTTGACGCGACGGCAAGTCAGGGACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGc < 1:2222546/65‑1 (MQ=255) aCGCGACGGCAAGTCAGGGACGGGCAATCACTGAACTCTTAACGTTACCCGCCGTAtt < 1:105841/58‑1 (MQ=255) cAAGTCAGGGACGGGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAgg < 1:1271817/69‑1 (MQ=255) aaGTCAGGGACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTa > 1:479988/1‑46 (MQ=255) aaGTCAGGGACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCa > 1:1842801/1‑58 (MQ=255) aaGTCAGGGACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAgt > 1:589786/1‑67 (MQ=255) aaGTCAGGGACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAgt > 1:605446/1‑67 (MQ=255) aaGTCAGGGACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAgt > 1:413355/1‑67 (MQ=255) aaGTCAGGGACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAgt > 1:1330681/1‑67 (MQ=255) aaGTCAGGGACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAgt > 1:319176/1‑67 (MQ=255) aaGTCAGGGACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAgt > 1:684003/1‑67 (MQ=255) aaGTCAGGGACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAgt > 1:3160736/1‑67 (MQ=255) aaGTCAGGGACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAgt > 1:3102226/1‑67 (MQ=255) aaGTCAGGGACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAgt > 1:725514/1‑67 (MQ=255) aaGTCAGGGACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAgt > 1:1384008/1‑67 (MQ=255) aaGTCAGGGACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAgt > 1:1419155/1‑67 (MQ=255) aaGTCAGGGACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAgt > 1:2707744/1‑67 (MQ=255) aaGTCAGGGACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAgt > 1:3047804/1‑67 (MQ=255) aaGTCAGGGACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAgt > 1:2876965/1‑67 (MQ=255) aaGTCAGGGACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAgt > 1:1775074/1‑67 (MQ=255) cAGGGACGGGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAGGcaca > 1:3086086/1‑68 (MQ=255) cAGGGACGGGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAGGcaca > 1:312842/1‑68 (MQ=255) cAGGGACGGGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAGGcaca > 1:335680/1‑68 (MQ=255) cAGGGACGGGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAGGCACAt > 1:1371617/1‑69 (MQ=255) cAGGGACGGGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAGGCACAt > 1:4993/1‑69 (MQ=255) cAGGGACGGGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAGGCACAt > 1:2757087/1‑69 (MQ=255) cAGGGACGGGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAGGCACAt > 1:2796872/1‑69 (MQ=255) cAGGGACGGGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAGGCACAt > 1:1201772/1‑69 (MQ=255) cAGGGACGGGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAGGCACAt > 1:2217507/1‑69 (MQ=255) aGGGACGGGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAGGCACATc < 1:1025030/69‑1 (MQ=255) aGGGACGGGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAGGCACATc < 1:1259902/69‑1 (MQ=255) ggACGGGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAAGAAGCAATAAAACAGGCACATCCg < 1:1359683/69‑1 (MQ=255) ggACGGGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCg < 1:1774821/69‑1 (MQ=255) gACGAGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGc < 1:3172390/42‑1 (MQ=255) ggCAATCACTGAACTCTTAACGTTACCCGCCGTAtt < 1:3039700/36‑1 (MQ=255) | TTGCAATTTATTAATGCCCAGGCGGCGCGGGTCTTGCGCCTTGACGCGACGGCAAGTCAGGGACGGGCAATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCCG > minE/803952‑804080 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |