| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 78,739 | A→C | 37.9% | E278D (GAA→GAC) | murE → | UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate:meso‑ diaminopimelate ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 78,739 | 0 | A | C | 37.9% | 61.0 / 107.4 | 116 | E278D (GAA→GAC) | murE | UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate:meso‑ diaminopimelate ligase |
| Reads supporting (aligned to +/- strand): ref base A (57/15); new base C (10/34); total (67/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.27e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 116 total aligned reads displayed.
TTGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGCTTTAGCTCAAGTTGGGGCGATGGCGAAATT > minE/78675‑78802 | ttGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGa < 1:2527065/69‑1 (MQ=255)ttGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGa < 1:2134560/69‑1 (MQ=255)ttGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGa < 1:2955001/69‑1 (MQ=255)ttGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGa < 1:648582/69‑1 (MQ=255)ttGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGa < 1:3314140/69‑1 (MQ=255)ttGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGa < 1:485643/69‑1 (MQ=255) tGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGa < 1:2453648/68‑1 (MQ=255) tGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGa < 1:3182933/68‑1 (MQ=255) tttCAATGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACt < 1:2989467/64‑1 (MQ=255) tATCAATGGAAGATCATATCAATCCGAACTGTCACGGACGCTTGTTGAAAGCGACCGACGTGAACt < 1:3220212/66‑1 (MQ=255) tATCAATGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACt < 1:1128825/66‑1 (MQ=255) tATCAATGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACt < 1:2335882/66‑1 (MQ=255) tATCAATGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACt < 1:2532731/66‑1 (MQ=255) tATCAATGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACt < 1:2656285/66‑1 (MQ=255) tATCAATGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACt < 1:202632/66‑1 (MQ=255) tATCAATGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACt < 1:3127287/66‑1 (MQ=255) tATCAATGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACt < 1:1652857/66‑1 (MQ=255) tATCAATGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACt < 1:1551625/66‑1 (MQ=255) tATCAATGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACt < 1:1415905/66‑1 (MQ=255) tATCAATGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACt < 1:1512196/66‑1 (MQ=255) aTCAATGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACt < 1:1602527/65‑1 (MQ=255) tCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCAc > 1:3429244/1‑69 (MQ=255) aaTGGATGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACTATCAc > 1:643/1‑67 (MQ=255) aaTGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCAc > 1:2307748/1‑67 (MQ=255) aaTGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCAc > 1:140527/1‑67 (MQ=255) aaTGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCAc > 1:982987/1‑67 (MQ=255) aaTGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCAc > 1:2078403/1‑67 (MQ=255) aaTGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCAc > 1:2035680/1‑67 (MQ=255) aaTGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCAc > 1:648595/1‑67 (MQ=255) aaTGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCAc > 1:1536722/1‑67 (MQ=255) aaTGGAAGATCATATTAATCCGAACTGTCA‑GGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGAc > 1:1972871/1‑69 (MQ=255) aaTGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACTATCAc > 1:854083/1‑67 (MQ=255) aaTGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACTATCAc > 1:2673934/1‑67 (MQ=255) aaTGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACTATCAc > 1:832200/1‑67 (MQ=255) aaTGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACTATCAc > 1:800661/1‑67 (MQ=255) aaTGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACTATCAc > 1:360024/1‑67 (MQ=255) aaTGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACTATCAc > 1:1353118/1‑67 (MQ=255) aaTGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACTATCAc > 1:17513/1‑67 (MQ=255) aaTGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGACGTGAACTATCAc > 1:1978736/1‑67 (MQ=255) aaTGGAAGATCATATCAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACAGACGTGAACTATCAc > 1:259788/1‑67 (MQ=255) gAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTAt > 1:2337353/1‑60 (MQ=255) gAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTAt > 1:1485784/1‑60 (MQ=255) gAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTAt > 1:687971/1‑60 (MQ=255) gAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTAt > 1:524526/1‑60 (MQ=255) gAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTAt > 1:3429936/1‑60 (MQ=255) gAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTAt > 1:3274918/1‑60 (MQ=255) tCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGc > 1:2485892/1‑69 (MQ=255) cATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCTAAGTGAACTATCACGACAGCGGTGcg > 1:442973/1‑69 (MQ=255) cATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGcg > 1:3325574/1‑69 (MQ=255) cATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGcg > 1:710699/1‑69 (MQ=255) cATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGcg > 1:1621233/1‑69 (MQ=255) cATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGcg > 1:2121828/1‑69 (MQ=255) cATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGcg > 1:919314/1‑69 (MQ=255) cATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGcg > 1:2714882/1‑69 (MQ=255) cATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGcg > 1:2705210/1‑69 (MQ=255) cATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGcg > 1:2174981/1‑69 (MQ=255) cATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGc > 1:518309/1‑68 (MQ=255) ttAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGcgacga > 1:2542290/1‑69 (MQ=255) ttAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGcgacg > 1:2333493/1‑68 (MQ=255) cGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGc > 1:7663/1‑68 (MQ=255) cGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGc > 1:1897334/1‑68 (MQ=255) cGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGc > 1:165386/1‑68 (MQ=255) cGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGc > 1:3094674/1‑68 (MQ=255) aaCTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGCttt < 1:992882/69‑1 (MQ=255) gTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGCTTTAGc > 1:565074/1‑68 (MQ=255) gTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGCTTTAGc > 1:568928/1‑68 (MQ=255) aCGGACGCTGGTTGAAAGCGACCGACGTGAACTATCACTACAGCGGTGCGACCATTCGCTTTAGCTc < 1:1840372/67‑1 (MQ=255) aCGGACGCTGGTTGAAAGCGACCGACGTGAACTATCACGACAGCGGTGCGACCATTCGCTTTAGCTc < 1:2609511/67‑1 (MQ=255) aCGGACGCTGGTTGAAAGCGACCGACGTGAACTATCACGACAGCGGTGCGACCATTCGCTTTAGCTc < 1:1687030/67‑1 (MQ=255) aCGGACGCTGGTTGAAAGCGACCGACGTGAACTATCACGACAGCGGTGCGACCATTCGCTTTAGCTc < 1:588071/67‑1 (MQ=255) aCGGACGCTGGTTGAAAGCGACCGACGTGAACTATCACGACAGCGGTGCGACCATTCGCTTTAGCTc < 1:2574305/67‑1 (MQ=255) aCGGACGCTGGTTGAAAGCGACCGACGTGAACTATCACGACAGCGGTGCGACCATTCGCTTTAGCTc < 1:1518066/67‑1 (MQ=255) aCGGACGCTGGTTGAAAGCGACCGACGTGAACTATCACGACAGCGGTGCGACCATTCGCTTTAGCTc < 1:679383/67‑1 (MQ=255) aCGGACGCTGGTTGAAAGCGACCGACGTGAACTATCACGACAGCGGTGCGACCATTCGCTTTAGCTc < 1:3135840/67‑1 (MQ=255) aCGGACGCTGGTTGAAAGCGACCGACGTGAACTATCACGACAGCGGTGCGACCATTCGCTTTAGCTc < 1:3087737/67‑1 (MQ=255) aCGGACGCTGGTTGAAAGCGACCGACGTGAACTATCACGACAGCGGTGCGACCATTCGCTTTAGCTc < 1:715529/67‑1 (MQ=255) aCGGACGCTGGTTGAAAGCGACCGACGTGAACTATCACGACAGCGGTGCGACCATTCGCTTTAGCTc < 1:3041765/67‑1 (MQ=255) aCGGACGCTGGTTGAAAGCGACCGACGTGAACTATCACGACAGCGGTGCGACCATTCGCTTTAGCTc < 1:1321199/67‑1 (MQ=255) aCGGACGCTGGTTGAAAGCGACCGACGTGAACTATCACGACAGCGGTGCGACCATTCGCTTTAGCTc < 1:2854883/67‑1 (MQ=255) aCGGACGCTGGTTGAAAGCGACCGACGTGAACTATCACGACAGCGGTGCGACCATTCGCTTTAGCTc < 1:92580/67‑1 (MQ=255) cGGACGCTGGTTGAAAGCGACCGACGTGAACTATCACGACAGCGGTGCGACAATTCGCTTTAGCTc < 1:1746965/66‑1 (MQ=255) cTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGCTTTAGCTCAAGTTggg > 1:88602/1‑68 (MQ=255) cTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGCTTTAGCTCAAGTTggg > 1:359592/1‑68 (MQ=255) cTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGCTTTAGCTCAAGTTggg > 1:2227373/1‑68 (MQ=255) cTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGCTTTAGCTCAAGTTggg > 1:1967073/1‑68 (MQ=255) cTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGCTTTAGCTCAAGTTggg > 1:1072180/1‑68 (MQ=255) cTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGCTTTAGCTCAAGTTggg > 1:3043270/1‑68 (MQ=255) cTGGCTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGCTTTAGCTCAAGTTggg > 1:2751619/1‑68 (MQ=255) tGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGCTTTAGCTCAAGTTGGGGc < 1:2627904/69‑1 (MQ=255) ggTTGAGAGCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGCTTTAGCTCAAGt > 1:439786/1‑62 (MQ=255) ggTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGCTTTAGCTCAAGTTGGGGc < 1:1205874/68‑1 (MQ=255) ggTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGCTTTAGCTCAAGTTGGGGCg > 1:260058/1‑69 (MQ=255) ggTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGCTTTAGCTCAAGTTGGGGCg > 1:2075583/1‑69 (MQ=255) ggTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGCTTTAGCTCAAGTTGGGGCg > 1:910938/1‑69 (MQ=255) ggTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGCTTTAGCTCAAGTTGGGGCg > 1:3141204/1‑69 (MQ=255) ggTTGAAAGCGACAGAAGTTAACTATCACGACAGCGGTGCGACGATTCGCTTTAGCTCAAGTTGGGGc < 1:2511721/68‑1 (MQ=255) gCGACCGACGTGAACTATCACGACAGCGGTGCGACCATTCGCTTTAGCTc < 1:2498265/50‑1 (MQ=255) gCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGCTTTAGCTCAAGTTGGGGc < 1:2508043/60‑1 (MQ=255) gCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGCTTTAGCTCAAGTTGGGGCGATGGCGaa > 1:2808998/1‑69 (MQ=255) cGAAGTGAACTATCACGACAGCGGTGCGACGATTCGCTTTAGCTCAAGTTGGGGCGATGGCGAAAtt < 1:29054/67‑1 (MQ=255) | TTGCGGTATCAATGGAAGATCATATTAATCCGAACTGTCACGGACGCTGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGCTTTAGCTCAAGTTGGGGCGATGGCGAAATT > minE/78675‑78802 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |