| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 804,215 | G→A | 28.2% | L312L (TTG→TTA) | dhaR → | predicted DNA‑binding transcriptional regulator, dihydroxyacetone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 804,215 | 0 | G | A | 28.2% | 78.7 / 29.7 | 74 | L312L (TTG→TTA) | dhaR | predicted DNA‑binding transcriptional regulator, dihydroxyacetone |
| Reads supporting (aligned to +/- strand): ref base G (29/24); new base A (8/13); total (37/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCG > minE/804158‑804283 | aaCGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCa > 1:1210187/1‑69 (MQ=255)aaCGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCa > 1:2727226/1‑69 (MQ=255)aaCGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCa > 1:3190067/1‑69 (MQ=255) tttATTTTGTTGCTCCATCCTGTGGAACAGATGCTGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGc < 1:1867892/69‑1 (MQ=255) tttATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGc < 1:994069/69‑1 (MQ=255) tttATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGc < 1:2750352/69‑1 (MQ=255) tttATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGc < 1:1004082/69‑1 (MQ=255) tttATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGc < 1:2711484/69‑1 (MQ=255) tttATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGc < 1:263358/69‑1 (MQ=255) tttATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGc < 1:2463569/69‑1 (MQ=255) tttATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGc < 1:302274/69‑1 (MQ=255) tttATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGc < 1:3031892/69‑1 (MQ=255) tttATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGc < 1:2176416/69‑1 (MQ=255) tttATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGc < 1:1737134/69‑1 (MQ=255) tttATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGc < 1:914781/69‑1 (MQ=255) tttATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGc < 1:1401142/69‑1 (MQ=255) tttATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGc < 1:378590/69‑1 (MQ=255) tttATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGc < 1:1519781/69‑1 (MQ=255) tttATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGc < 1:1442502/69‑1 (MQ=255) tttATCTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGc < 1:2230800/69‑1 (MQ=255) ttttGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCAt < 1:2871076/68‑1 (MQ=255) ttttGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCAt < 1:3070977/68‑1 (MQ=255) tCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCatat > 1:2836129/1‑69 (MQ=255) tCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCatat > 1:3210171/1‑69 (MQ=255) tCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCatat > 1:673834/1‑69 (MQ=255) ggAACAGATGCGGCAGTTAATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCcac > 1:1592483/1‑68 (MQ=255) ggAACAGATGCGGCAGTTAATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCcac > 1:79243/1‑68 (MQ=255) ggAACAGATGCGGCAGTTAATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCcac > 1:55910/1‑68 (MQ=255) ggAACAGATGCGGCAGTTAATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCcac > 1:1749655/1‑68 (MQ=255) ggAACAGATGCGGCAGTTAATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCcac > 1:1624386/1‑68 (MQ=255) ggAACAGATGCGGCAGTTAATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCcac > 1:1572253/1‑68 (MQ=255) ggAACAGATGCGGCAGTTAATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCca > 1:577448/1‑67 (MQ=255) ggAACAGATGCGGCAGTTAATGACCAGTCAATTAGGAAAACTCAGCCATAca > 1:3159996/1‑51 (MQ=255) gAACAGATGCGGCAGTTAATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCcac < 1:3080514/67‑1 (MQ=255) gAACAGATGCGGCAGTTAATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCcac < 1:2896398/67‑1 (MQ=255) gAACAGATGCGGCAGTTAATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCcac < 1:58742/67‑1 (MQ=255) gAACAGATGCGGCAGTTAATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCcac < 1:489031/67‑1 (MQ=255) gAACAGATGCGGCAGTTAATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCcac < 1:3218454/67‑1 (MQ=255) gAACAGATGCGGCAGTTAATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCcac < 1:3256244/67‑1 (MQ=255) gAACAGATGCGGCAGTTAATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCcac < 1:2736517/67‑1 (MQ=255) gAACAGATGCGGCAGTTAATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCcac < 1:1609012/67‑1 (MQ=255) gAACAGATGCGGCAGTTAATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCcac < 1:1178353/67‑1 (MQ=255) gAACAGATGCGGCAGTTAATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCcac < 1:1441713/67‑1 (MQ=255) gAACAGATGCGGCAGTTAATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCcac < 1:2780349/67‑1 (MQ=255) gAACAGATGCGGCAGTTAATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCcac < 1:2270519/67‑1 (MQ=255) gAACAGATGAGGCAGTTAATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCcac < 1:1297816/67‑1 (MQ=255) cAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCa > 1:344893/1‑56 (MQ=255) cAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCa > 1:2846378/1‑56 (MQ=255) cAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCa > 1:1413581/1‑56 (MQ=255) cAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCa > 1:744420/1‑56 (MQ=255) cAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCa > 1:721968/1‑56 (MQ=255) cAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCa > 1:1415969/1‑56 (MQ=255) cAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCa > 1:2710462/1‑56 (MQ=255) cAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCa > 1:1449754/1‑56 (MQ=255) cAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCa > 1:1455174/1‑56 (MQ=255) cAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCa > 1:1461410/1‑56 (MQ=255) cAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCa > 1:1569046/1‑56 (MQ=255) cAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCa > 1:2339402/1‑56 (MQ=255) cAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCa > 1:3337754/1‑56 (MQ=255) cAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCa > 1:3259775/1‑56 (MQ=255) cAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCa > 1:2390825/1‑56 (MQ=255) cAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCa > 1:1930993/1‑56 (MQ=255) cAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCa > 1:1961480/1‑56 (MQ=255) cAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCa > 1:1977147/1‑56 (MQ=255) cAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCa > 1:3111075/1‑56 (MQ=255) cAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCa > 1:3097932/1‑56 (MQ=255) cAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCa > 1:2237195/1‑56 (MQ=255) cAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCa > 1:2243870/1‑56 (MQ=255) cAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCa > 1:3006481/1‑56 (MQ=255) gCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCa < 1:3253960/51‑1 (MQ=255) gCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCa < 1:550093/51‑1 (MQ=255) gCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCa < 1:2079079/51‑1 (MQ=255) gCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCa < 1:3119940/51‑1 (MQ=255) gCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCa < 1:785386/51‑1 (MQ=255) gatgaCCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACccg > 1:477455/1‑69 (MQ=255) | AACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCG > minE/804158‑804283 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |