BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	811,407	A→T	62.0%	A537A (GCT→GCA)	ychM ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	811,407	0	A	T	62.0%	32.5 / 42.7	50	A537A (GCT→GCA)	ychM	predicted transporter
Reads supporting (aligned to +/- strand): ref base A (9/10); new base T (22/9); total (31/19)
Fisher's exact test for biased strand distribution p-value = 1.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCATCGCCGCGCGACGATTCGGGAAGAACGCCAGGCGTCCCGGGATCGGTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCACGTTGCACACGCGCAGTTCACATCCCTC  >  minE/811349‑811469

ccATCGCCGCGCGACGATTCGGGAAGAACGCCAGGCGTCCCGGGATCGGTTGAATGCCAGCGCGAGCCa                                                      <  1:2706872/69‑1 (MQ=255)

ccATCGCCGCGCGACGATTCGGGAAGAACGCCAGGCGTCCCGGGATCGGTTGAATGCCAGCGCGAGCCa                                                      <  1:2505397/69‑1 (MQ=255)

ccATCGCCGCGCGACGATTCGGGAAGAACGCCAGGCGTCCCGGGATCGGTTGAATGCCAGCGCGAGCCa                                                      <  1:2225632/69‑1 (MQ=255)

ccATCGCCGCGCGACGATTCGGGAAGAACGCCAGGCGTCCCGGGATCGGTTGAATGCCAGCGCGAGCCa                                                      <  1:1816601/69‑1 (MQ=255)

     gccgcGCGACGATTCGGGAAGAACGCCAGGCGTCCCGGGATCGGTTGAATGCCAGCGCGAGCCATAGTg                                                 >  1:1046398/1‑69 (MQ=255)

     gccgcGCGACGATTCGGGAAGAACGCCAGGCGTCCCGGGATCGGTTGAATGCCAGCGCGAGCCATAGTg                                                 >  1:2499188/1‑69 (MQ=255)

     gccgcGCGACGATTCGGGAAGAACGCCAGGCGTCCCGGGATCGGTTGAATGCCAGCGCGAGCCATAGTg                                                 >  1:764209/1‑69 (MQ=255)

     gccgcGCGACGATTCGGGAAGAACGCCAGGCGTCCCGGGATCGGTTGAATGCCAGCGCGAGCCATAGTg                                                 >  1:1417023/1‑69 (MQ=255)

     gccgcGCGACGATTCGGGAAGAACGCCAGGCGTCCCGGGATCGGTTGAATGCCAGCGCGAGCCATAGTg                                                 >  1:81835/1‑69 (MQ=255)

                  tCGGGAAGAACGCCAGGCGTCCCGGGATCGGTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTgg                                     <  1:428227/68‑1 (MQ=255)

                        agaaCGCCAGGCGTCCCGGGATCGGTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCa                              <  1:805934/69‑1 (MQ=255)

                        agaaCGCCAGGCGTCCCGGGATCGGTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCa                              <  1:2767968/69‑1 (MQ=255)

                        agaaCGCCAGGCGTCCCGGGATCGGTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCa                              <  1:2479799/69‑1 (MQ=255)

                         gaaCGCCAGGCGTCCCGGGATCGGTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAc                             >  1:1930259/1‑69 (MQ=255)

                         gaaCGCCAGGCGTCCCGGGATCGGTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAc                             >  1:3081778/1‑69 (MQ=255)

                                    gTCCCGGGATCGGTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCACGTTGCACAcg                   >  1:2155075/1‑68 (MQ=255)

                                            aTCGGTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCACGTTGCACACGCGCAGTTca          <  1:2601455/69‑1 (MQ=255)

                                            aTCGGTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCACGTTGCACACGCGCAGTTca          >  1:962138/1‑69 (MQ=255)

                                                  tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGt                           <  1:2404616/46‑1 (MQ=39)

                                                  tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCCCACGCGCAGTTCACATcct    >  1:815582/1‑68 (MQ=255)

                                                  tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTca          >  1:2841273/1‑63 (MQ=255)

                                                  tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct    >  1:3016964/1‑68 (MQ=255)

                                                  tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct    >  1:3099434/1‑68 (MQ=255)

                                                  tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct    >  1:1060685/1‑68 (MQ=255)

                                                  tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct    >  1:3153909/1‑68 (MQ=255)

                                                  tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct    >  1:428529/1‑68 (MQ=255)

                                                  tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct    >  1:515013/1‑68 (MQ=255)

                                                  tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct    >  1:564105/1‑68 (MQ=255)

                                                  tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct    >  1:718547/1‑68 (MQ=255)

                                                  tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct    >  1:733950/1‑68 (MQ=255)

                                                  tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct    >  1:2253416/1‑68 (MQ=255)

                                                  tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct    >  1:113520/1‑68 (MQ=255)

                                                  tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct    >  1:1272455/1‑68 (MQ=255)

                                                  tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct    >  1:1514455/1‑68 (MQ=255)

                                                  tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct    >  1:1561838/1‑68 (MQ=255)

                                                  tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct    >  1:2035798/1‑68 (MQ=255)

                                                  tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct    >  1:215066/1‑68 (MQ=255)

                                                  tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct    >  1:2288927/1‑68 (MQ=255)

                                                  tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct    >  1:249905/1‑68 (MQ=255)

                                                  tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGAAGTTCACATcct    >  1:3241280/1‑68 (MQ=255)

                                                  tGAATGACTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct    >  1:439268/1‑68 (MQ=255)

                                                     aTGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCTAGGTTGCACACGCGCAGTTCACATCCTTc  <  1:130949/68‑1 (MQ=255)

                                                     aTGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTc  <  1:298004/68‑1 (MQ=255)

                                                     aTGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTc  <  1:2627626/68‑1 (MQ=255)

                                                     aTGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTc  <  1:273654/68‑1 (MQ=255)

                                                     aTGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTc  <  1:2778946/68‑1 (MQ=255)

                                                     aTGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTc  <  1:2971945/68‑1 (MQ=255)

                                                      tGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTc  <  1:1388054/67‑1 (MQ=255)

                                                      tGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTc  <  1:907977/67‑1 (MQ=255)

                                                        ccAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCa                              <  1:393103/37‑1 (MQ=255)

CCATCGCCGCGCGACGATTCGGGAAGAACGCCAGGCGTCCCGGGATCGGTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCACGTTGCACACGCGCAGTTCACATCCCTC  >  minE/811349‑811469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`