Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	811,442	C→G	70.4%	V526L (GTG→CTG)	ychM ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	811,442	0	C	G	70.4%	83.2 / 45.9	71	V526L (GTG→CTG)	ychM	predicted transporter
Reads supporting (aligned to +/- strand):  ref base C (17/4);  new base G (29/21);  total (46/25)
Fisher's exact test for biased strand distribution p-value = 1.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01

GAACGCCAGGCGTCCCGGGATCGGTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCACGTTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACGCTGGAACGCATCAAGAC  >  minE/811374‑811507                                                                     |                                                                  gaaCGCCAGGCGTCCCGGGATCGGTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAc                                                                   >  1:1930259/1‑69 (MQ=255) gaaCGCCAGGCGTCCCGGGATCGGTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAc                                                                   >  1:3081778/1‑69 (MQ=255)            gTCCCGGGATCGGTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCACGTTGCACAcg                                                         >  1:2155075/1‑68 (MQ=255)                    aTCGGTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCACGTTGCACACGCGCAGTTca                                                >  1:962138/1‑69 (MQ=255)                    aTCGGTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCACGTTGCACACGCGCAGTTca                                                <  1:2601455/69‑1 (MQ=255)                          tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGt                                                                 <  1:2404616/46‑1 (MQ=39)                          tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCCCACGCGCAGTTCACATcct                                          >  1:815582/1‑68 (MQ=255)                          tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTca                                                >  1:2841273/1‑63 (MQ=255)                          tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct                                          >  1:515013/1‑68 (MQ=255)                          tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct                                          >  1:564105/1‑68 (MQ=255)                          tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct                                          >  1:1060685/1‑68 (MQ=255)                          tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct                                          >  1:3016964/1‑68 (MQ=255)                          tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct                                          >  1:249905/1‑68 (MQ=255)                          tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct                                          >  1:718547/1‑68 (MQ=255)                          tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct                                          >  1:3099434/1‑68 (MQ=255)                          tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct                                          >  1:733950/1‑68 (MQ=255)                          tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct                                          >  1:2288927/1‑68 (MQ=255)                          tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct                                          >  1:2253416/1‑68 (MQ=255)                          tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct                                          >  1:3153909/1‑68 (MQ=255)                          tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct                                          >  1:428529/1‑68 (MQ=255)                          tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct                                          >  1:215066/1‑68 (MQ=255)                          tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct                                          >  1:113520/1‑68 (MQ=255)                          tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct                                          >  1:2035798/1‑68 (MQ=255)                          tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct                                          >  1:1561838/1‑68 (MQ=255)                          tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct                                          >  1:1272455/1‑68 (MQ=255)                          tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct                                          >  1:1514455/1‑68 (MQ=255)                          tGAATGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGAAGTTCACATcct                                          >  1:3241280/1‑68 (MQ=255)                          tGAATGACTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATcct                                          >  1:439268/1‑68 (MQ=255)                             aTGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCTAGGTTGCACACGCGCAGTTCACATCCTTc                                        <  1:130949/68‑1 (MQ=255)                             aTGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTc                                        <  1:298004/68‑1 (MQ=255)                             aTGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTc                                        <  1:2971945/68‑1 (MQ=255)                             aTGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTc                                        <  1:2778946/68‑1 (MQ=255)                             aTGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTc                                        <  1:273654/68‑1 (MQ=255)                             aTGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTc                                        <  1:2627626/68‑1 (MQ=255)                              tGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTc                                        <  1:907977/67‑1 (MQ=255)                              tGCCTGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTc                                        <  1:1388054/67‑1 (MQ=255)                                   gcgcGAGCCATAGTGCGCAGTGGCTGGAATTCCACGTTGCACACGCGCAGTTCACATCCCTCGTGCAGa                                 <  1:2292892/69‑1 (MQ=255)                                   gcgcGAGCCATAGTGCGCAGTGGCTGGAATTCCACGTTGCACACGCGCAGTTCACATCCCTCGGGCAGa                                 <  1:1216256/69‑1 (MQ=255)                                   gcgcGAGCCATAGTGCGCAGTGGCTGGAATTCCACGTTGCACACGCGCAGTTCACATCCCTCGGGCAGa                                 <  1:2396517/69‑1 (MQ=255)                                    cgcgAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTCGGGCAGAc                                >  1:878146/1‑69 (MQ=255)                                    cgcgAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTCGGGCAGAc                                >  1:118726/1‑69 (MQ=255)                                    cgcgAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTCGGGCAGAc                                >  1:850948/1‑69 (MQ=255)                                    cgcgAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTCGGGCAGAc                                >  1:1670358/1‑69 (MQ=255)                                    cgcgAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTCGGGCAGAc                                >  1:462102/1‑69 (MQ=255)                                    cgcgAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTCGGGCAGAc                                >  1:3332086/1‑69 (MQ=255)                                    cgcgAGCCATAGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTCGGGCAGAc                                >  1:2979344/1‑69 (MQ=255)                                              aGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTCGGGCAGACGCTTCACaaa                      <  1:3198329/69‑1 (MQ=255)                                              aGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTCGGGCAGACGCTTCACaaa                      <  1:2080926/69‑1 (MQ=255)                                              aGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTCGGGCAGACGCTTCACaaa                      <  1:1416325/69‑1 (MQ=255)                                              aGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTCGGGCAGACGCTTCACaaa                      <  1:2824585/69‑1 (MQ=255)                                              aGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTCGGGCAGACGCTTCACaaa                      <  1:1439668/69‑1 (MQ=255)                                              aGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTCGGGCAGACGCTTCACaaa                      <  1:2670040/69‑1 (MQ=255)                                              aGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTCGGGCAGACGCTTCACaaa                      <  1:1478372/69‑1 (MQ=255)                                              aGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTCGGGCAGACGCTTCACaaa                      <  1:1528543/69‑1 (MQ=255)                                              aGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTCGGGCAGACGCTTCACaaa                      <  1:2312572/69‑1 (MQ=255)                                              aGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTCGGGCAGACGCTTCACaaa                      <  1:1680999/69‑1 (MQ=255)                                              aGTGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTCGGGCAGACGCTTCACaaa                      <  1:1970626/69‑1 (MQ=255)                                                tGCGCAGTGGCTGGAATTCCAGGTTGCACACGCGCAGTTCACATCCTTCGGGCAGACGCTTCACaaa                      <  1:2625741/67‑1 (MQ=255)                                                 gcgcAGTGGCTGGAATTCCACGTTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACg                   >  1:3341928/1‑69 (MQ=255)                                                 gcgcAGTGGCTGGAATTCCACGTTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACg                   >  1:626793/1‑69 (MQ=255)                                                 gcgcAGTGGCTGGAATTCCACGTTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACg                   >  1:2428617/1‑69 (MQ=255)                                                 gcgcAGTGGCTGGAATTCCACGTTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACg                   >  1:2023617/1‑69 (MQ=255)                                                 gcgcAGTGGCTGGAATTCCACGTTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACg                   >  1:1453585/1‑69 (MQ=255)                                                 gcgcAGTGGCTGGAATTCCACGTTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACg                   >  1:988150/1‑69 (MQ=255)                                                       tGGCTGGAATTCCACGTTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACGCTGGaa             >  1:3065935/1‑69 (MQ=255)                                                       tGGCTGGAATTCCACGTTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACGCTGGaa             >  1:2987467/1‑69 (MQ=255)                                                       tGGCTGGAATTCCACGTTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACGCTGGaa             >  1:2947900/1‑69 (MQ=255)                                                       tGGCTGGAATTCCACGTTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACGCTGGaa             >  1:2368259/1‑69 (MQ=255)                                                       tGGCTGGAATTCCACGTTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACGCTGGaa             >  1:1632060/1‑69 (MQ=255)                                                       tGGCTGGAATTCCACGTTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACGCTGGaa             >  1:870742/1‑69 (MQ=255)                                                       tGGCTGGAATTCCACGTTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACGCTGGaa             >  1:1517960/1‑69 (MQ=255)                                                       tGGCTGGAATTCCACGTTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACGCTGGaa             >  1:1240633/1‑69 (MQ=255)                                                                  ccACGTTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACGCTGGAACGCATCAAGac  >  1:275480/1‑69 (MQ=255)                                                                     |                                                                  GAACGCCAGGCGTCCCGGGATCGGTTGAATGCCAGCGCGAGCCATAGTGCGCAGTGGCTGGAATTCCACGTTGCACACGCGCAGTTCACATCCCTCGGGCAGACGCTTCACAAAACGCTGGAACGCATCAAGAC  >  minE/811374‑811507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑